DNA polymerase I is not required for replication of linear chromosomes in Streptomyces

Tzu-Wen Huang; Hsiu-Hwei Chen

doi:10.1128/JB.01335-07

DNA polymerase I is not required for replication of linear chromosomes in Streptomyces

Tzu-Wen Huang, Hsiu-Hwei Chen

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Both pol4 (encoding DNA polymerase I; Pol I) and a paralog were deleted from Streptomyces strains. Despite the UV sensitivity and slow growth caused by the ΔpolA mutation, the double mutant was viable. Thus, in contrast to a previous postulate, Pol I and its paralog are not essential for replication of Streptomyces chromosomes. Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Original language	English
Pages (from-to)	755-758
Number of pages	4
Journal	Journal of Bacteriology
Volume	190
Issue number	2
DOIs	https://doi.org/10.1128/JB.01335-07
Publication status	Published - 2008
Externally published	Yes

Keywords

bacterial DNA
DNA directed DNA polymerase beta
article
bacterial chromosome
biosynthesis
gene deletion
gene expression regulation
genetics
growth, development and aging
metabolism
microbial viability
radiation exposure
Streptomyces
ultraviolet radiation
bacterial growth
bacterial strain
bacterium mutant
chromosome replication
gene mutation
nonhuman
priority journal
radiosensitivity
Chromosomes, Bacterial
DNA Polymerase I
DNA, Bacterial
Gene Deletion
Microbial Viability
Mutagenesis, Insertional
Ultraviolet Rays

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10.1128/JB.01335-07

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Cite this

@article{edf3651d953d47649d9d5c406e903d4f,

title = "DNA polymerase I is not required for replication of linear chromosomes in Streptomyces",

abstract = "Both pol4 (encoding DNA polymerase I; Pol I) and a paralog were deleted from Streptomyces strains. Despite the UV sensitivity and slow growth caused by the ΔpolA mutation, the double mutant was viable. Thus, in contrast to a previous postulate, Pol I and its paralog are not essential for replication of Streptomyces chromosomes. Copyright {\textcopyright} 2008, American Society for Microbiology. All Rights Reserved.",

keywords = "bacterial DNA, DNA directed DNA polymerase beta, article, bacterial chromosome, biosynthesis, gene deletion, gene expression regulation, genetics, growth, development and aging, metabolism, microbial viability, radiation exposure, Streptomyces, ultraviolet radiation, bacterial growth, bacterial strain, bacterium mutant, chromosome replication, gene mutation, nonhuman, priority journal, radiosensitivity, Chromosomes, Bacterial, DNA Polymerase I, DNA, Bacterial, Gene Deletion, Microbial Viability, Mutagenesis, Insertional, Ultraviolet Rays",

author = "Tzu-Wen Huang and Hsiu-Hwei Chen",

note = "被引用次數：8 Export Date: 12 April 2016 CODEN: JOBAA 通訊地址: Chen, C. W.; Department of Life Sciences, Institute of Genome Sciences, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan; 電子郵件： [email protected] 化學物質/CAS: DNA Polymerase I, EC 2.7.7.-; DNA, Bacterial 參考文獻: Bao, K., Cohen, S.N., Recruitment of terminal protein to the ends of Streptomyces linear plasmids and chromosomes by a novel telomere-binding protein essential for linear DNA replication (2003) Genes Dev, 17, pp. 774-785; Bao, K., Cohen, S.N., Reverse transcriptase activity innate to DNA polymerase I and DNA topoisomerase I proteins of Streptomyces telomere complex (2004) Proc. Natl. Acad. Sci. USA, 101, pp. 14361-14366; Bao, K., Cohen, S.N., Terminal proteins essential for the replication of linear plasmids and chromosomes in Streptomyces (2001) Genes Dev, 15, pp. 1518-1527; Bentley, S. D., K. F. Chater, A.-M. Cerde{\~n}o-T{\'a}rraga, G. L. Challis, N. R. Thomson, K. D. James, D. E. Harris, M. A. Quail, H. Kieser, D. Harper, A. Bateman, S. Brown, G. Chandra, C. W. Chen, M. Collins, A. Cronin, A. Fraser, A. Goble, J. Hidalgo, T. Hornsby, S. Howarth, C. H. Huang, T. Kieser, L. Larke, L. Murphy, K. Oliver, S. O'Neil, E. Rabbinowitsch, M. A. Rajandream, K. Rutherford, S. Rutter, K. Seeger, D. Saunders, S. Sharp, R. Squares, S. Squares, K. Taylor, T. Warren, A. Wietzorrek, J. Woodward, B. G. Barrell, J. Parkhill, and D. A. Hopwood. 2002. Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). Nature 417:141-147Chang, P.C., Cohen, S.N., Bidirectional replication from an internal origin in a linear Streptomyces plasmid (1994) Science, 265, pp. 952-954; Chen, C.W., Complications and implications of linear bacterial chromosomes (1996) Trends Genet, 12, pp. 192-196; Gust, B., Challis, G.L., Fowler, K., Kieser, T., Chater, K.F., PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin (2003) Proc. Natl. Acad. Sci. USA, 100, pp. 1541-1546; Hopwood, D.A., Kieser, T., Wright, H.M., Bibb, M.J., Plasmids, recombination, and chromosomal mapping in Streptomyces lividans 66 (1983) J. Gen. Microbiol, 129, pp. 2257-2269; Huang, T.W., Chen, C.W., A recA null mutation may be generated in Streptomyces coelicolor (2006) J. Bacteriol, 188, pp. 6771-6779; Ikeda, H., Ishikawa, J., Hanamoto, A., Shinose, M., Kikuchi, H., Shiba, T., Sakaki, Y., Omura, S., Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis (2003) Nat Biotechnol, 21, pp. 526-531; Joyce, C.M., Grindley, N.D., Method for determining whether a gene of Escherichia coli is essential: Application to the polA gene (1984) J. Bacteriol, 158, pp. 636-643; Kieser, T., Bibb, M., Buttner, M.J., Chater, K.F., Hopwood, D.A., (2000) Practical Streptomyces genetics, , The John Innes Foundation, Norwich, United Kingdom; Laity, C., Chater, K.F., Lewis, C.G., Buttner, M.J., Genetic analysis of the φC31-specific phage growth limitation (Pgl) system of Streptomyces coelicolor A3(2) (1993) Mol. Microbiol, 7, pp. 329-336; Lee, L.-F., Chen, Y.-J., Kirby, R., Chen, C., Chen, C.W., A multidrug efflux system is involved in colony growth in Streptomyces lividans (2007) Microbiology, 153, pp. 924-934; Okazaki, R., Arisawa, M., Sugino, A., Slow joining of newly replicated DNA chains in DNA polymerase I-deficient Escherichia coli mutants (1971) Proc. Natl. Acad. Sci. USA, 68, pp. 2954-2957; Olivera, B.M., Bonhoeffer, F., Replication of Escherichia coli requires DNA polymerase I (1974) Nature, 250, pp. 513-514; Paget, M.S.B., Chamberlin, L., Atrih, A.M., Foster, S.J., Buttner, M.J., Evidence that the extracytoplasmic function sigma factor σ E is required for normal cell wall structure in Streptomyces coelicolor A3(2) (1999) J. Bacteriol, 181, pp. 204-211; Redenbach, M., Kieser, H.M., Denapaite, D., Eichner, A., Cullum, J., Kinashi, H., Hopwood, D.A., A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome (1996) Mol. Microbiol, 21, pp. 77-96; Weaver, D., Karoonuthaisiri, N., Tsai, H.H., Huang, C.H., Ho, M.L., Gai, S., Patel, K.G., Kao, C.M., Genome plasticity in Streptomyces: Identification of 1 Mb TIRs in the S. coelicolor A3(2) chromosome (2004) Mol. Microbiol, 51, pp. 1535-1550; Yang, C.-C., Huang, C.-H., Li, C.-Y., Tsay, Y.-G., Lee, S.-C., Chen, C.W., The terminal proteins of linear Streptomyces chromosomes and plasmids: A novel class of replication priming proteins (2002) Mol. Microbiol, 43, pp. 297-305; Yang, C.-C., Chen, Y.-H., Tsai, H.-H., Huang, C.-H., Huang, T.-W., Chen, C.W., In vitro deoxynucleotidylation of the terminal protein of Streptomyces linear chromosomes (2006) Appl. Environ. Microbiol, 72, pp. 7959-7961",

year = "2008",

doi = "10.1128/JB.01335-07",

language = "English",

volume = "190",

pages = "755--758",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "2",

}

TY - JOUR

T1 - DNA polymerase I is not required for replication of linear chromosomes in Streptomyces

AU - Huang, Tzu-Wen

AU - Chen, Hsiu-Hwei

N1 - 被引用次數：8 Export Date: 12 April 2016 CODEN: JOBAA 通訊地址: Chen, C. W.; Department of Life Sciences, Institute of Genome Sciences, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan; 電子郵件： [email protected] 化學物質/CAS: DNA Polymerase I, EC 2.7.7.-; DNA, Bacterial 參考文獻: Bao, K., Cohen, S.N., Recruitment of terminal protein to the ends of Streptomyces linear plasmids and chromosomes by a novel telomere-binding protein essential for linear DNA replication (2003) Genes Dev, 17, pp. 774-785; Bao, K., Cohen, S.N., Reverse transcriptase activity innate to DNA polymerase I and DNA topoisomerase I proteins of Streptomyces telomere complex (2004) Proc. Natl. Acad. Sci. USA, 101, pp. 14361-14366; Bao, K., Cohen, S.N., Terminal proteins essential for the replication of linear plasmids and chromosomes in Streptomyces (2001) Genes Dev, 15, pp. 1518-1527; Bentley, S. D., K. F. Chater, A.-M. Cerdeño-Tárraga, G. L. Challis, N. R. Thomson, K. D. James, D. E. Harris, M. A. Quail, H. Kieser, D. Harper, A. Bateman, S. Brown, G. Chandra, C. W. Chen, M. Collins, A. Cronin, A. Fraser, A. Goble, J. Hidalgo, T. Hornsby, S. Howarth, C. H. Huang, T. Kieser, L. Larke, L. Murphy, K. Oliver, S. O'Neil, E. Rabbinowitsch, M. A. Rajandream, K. Rutherford, S. Rutter, K. Seeger, D. Saunders, S. Sharp, R. Squares, S. Squares, K. Taylor, T. Warren, A. Wietzorrek, J. Woodward, B. G. Barrell, J. Parkhill, and D. A. Hopwood. 2002. Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). Nature 417:141-147Chang, P.C., Cohen, S.N., Bidirectional replication from an internal origin in a linear Streptomyces plasmid (1994) Science, 265, pp. 952-954; Chen, C.W., Complications and implications of linear bacterial chromosomes (1996) Trends Genet, 12, pp. 192-196; Gust, B., Challis, G.L., Fowler, K., Kieser, T., Chater, K.F., PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin (2003) Proc. Natl. Acad. Sci. USA, 100, pp. 1541-1546; Hopwood, D.A., Kieser, T., Wright, H.M., Bibb, M.J., Plasmids, recombination, and chromosomal mapping in Streptomyces lividans 66 (1983) J. Gen. Microbiol, 129, pp. 2257-2269; Huang, T.W., Chen, C.W., A recA null mutation may be generated in Streptomyces coelicolor (2006) J. Bacteriol, 188, pp. 6771-6779; Ikeda, H., Ishikawa, J., Hanamoto, A., Shinose, M., Kikuchi, H., Shiba, T., Sakaki, Y., Omura, S., Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis (2003) Nat Biotechnol, 21, pp. 526-531; Joyce, C.M., Grindley, N.D., Method for determining whether a gene of Escherichia coli is essential: Application to the polA gene (1984) J. Bacteriol, 158, pp. 636-643; Kieser, T., Bibb, M., Buttner, M.J., Chater, K.F., Hopwood, D.A., (2000) Practical Streptomyces genetics, , The John Innes Foundation, Norwich, United Kingdom; Laity, C., Chater, K.F., Lewis, C.G., Buttner, M.J., Genetic analysis of the φC31-specific phage growth limitation (Pgl) system of Streptomyces coelicolor A3(2) (1993) Mol. Microbiol, 7, pp. 329-336; Lee, L.-F., Chen, Y.-J., Kirby, R., Chen, C., Chen, C.W., A multidrug efflux system is involved in colony growth in Streptomyces lividans (2007) Microbiology, 153, pp. 924-934; Okazaki, R., Arisawa, M., Sugino, A., Slow joining of newly replicated DNA chains in DNA polymerase I-deficient Escherichia coli mutants (1971) Proc. Natl. Acad. Sci. USA, 68, pp. 2954-2957; Olivera, B.M., Bonhoeffer, F., Replication of Escherichia coli requires DNA polymerase I (1974) Nature, 250, pp. 513-514; Paget, M.S.B., Chamberlin, L., Atrih, A.M., Foster, S.J., Buttner, M.J., Evidence that the extracytoplasmic function sigma factor σ E is required for normal cell wall structure in Streptomyces coelicolor A3(2) (1999) J. Bacteriol, 181, pp. 204-211; Redenbach, M., Kieser, H.M., Denapaite, D., Eichner, A., Cullum, J., Kinashi, H., Hopwood, D.A., A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome (1996) Mol. Microbiol, 21, pp. 77-96; Weaver, D., Karoonuthaisiri, N., Tsai, H.H., Huang, C.H., Ho, M.L., Gai, S., Patel, K.G., Kao, C.M., Genome plasticity in Streptomyces: Identification of 1 Mb TIRs in the S. coelicolor A3(2) chromosome (2004) Mol. Microbiol, 51, pp. 1535-1550; Yang, C.-C., Huang, C.-H., Li, C.-Y., Tsay, Y.-G., Lee, S.-C., Chen, C.W., The terminal proteins of linear Streptomyces chromosomes and plasmids: A novel class of replication priming proteins (2002) Mol. Microbiol, 43, pp. 297-305; Yang, C.-C., Chen, Y.-H., Tsai, H.-H., Huang, C.-H., Huang, T.-W., Chen, C.W., In vitro deoxynucleotidylation of the terminal protein of Streptomyces linear chromosomes (2006) Appl. Environ. Microbiol, 72, pp. 7959-7961

PY - 2008

Y1 - 2008

N2 - Both pol4 (encoding DNA polymerase I; Pol I) and a paralog were deleted from Streptomyces strains. Despite the UV sensitivity and slow growth caused by the ΔpolA mutation, the double mutant was viable. Thus, in contrast to a previous postulate, Pol I and its paralog are not essential for replication of Streptomyces chromosomes. Copyright © 2008, American Society for Microbiology. All Rights Reserved.

AB - Both pol4 (encoding DNA polymerase I; Pol I) and a paralog were deleted from Streptomyces strains. Despite the UV sensitivity and slow growth caused by the ΔpolA mutation, the double mutant was viable. Thus, in contrast to a previous postulate, Pol I and its paralog are not essential for replication of Streptomyces chromosomes. Copyright © 2008, American Society for Microbiology. All Rights Reserved.

KW - bacterial DNA

KW - DNA directed DNA polymerase beta

KW - article

KW - bacterial chromosome

KW - biosynthesis

KW - gene deletion

KW - gene expression regulation

KW - genetics

KW - growth, development and aging

KW - metabolism

KW - microbial viability

KW - radiation exposure

KW - Streptomyces

KW - ultraviolet radiation

KW - bacterial growth

KW - bacterial strain

KW - bacterium mutant

KW - chromosome replication

KW - gene mutation

KW - nonhuman

KW - priority journal

KW - radiosensitivity

KW - Chromosomes, Bacterial

KW - DNA Polymerase I

KW - DNA, Bacterial

KW - Gene Deletion

KW - Microbial Viability

KW - Mutagenesis, Insertional

KW - Ultraviolet Rays

U2 - 10.1128/JB.01335-07

DO - 10.1128/JB.01335-07

M3 - Article

SN - 0021-9193

VL - 190

SP - 755

EP - 758

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 2

ER -

DNA polymerase I is not required for replication of linear chromosomes in Streptomyces

Abstract

Keywords

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