DNA methyltransferase Dnmt1 and mismatch repair

Keh Yang Wang; C. K.James Shen

doi:10.1038/sj.onc.1208111

DNA methyltransferase Dnmt1 and mismatch repair

Keh Yang Wang, C. K.James Shen

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

We have examined the relationship between DNA mismatch repair and deficiency of DNA methylation in a mouse embryonic cell line, Dnmt1 ^-/- ES, with homologous deletion of the gene coding for the maintenance DNA methyltransferase Dnmt1. With the use of a sensitive PCR for the assay of two mononucleotide- and three dinucleotide microsatellite markers that exhibited instabilities in mismatch repair-deficient cells, significantly higher frequencies of instability were detected at three of the five markers in Dnmt1^-/- ES than the wild-type ES. The data suggest that Dnmt1 enzyme plays an integrating role in DNA replication and/or repair. The implication that Dnmt1 enzyme and/or cytosine methylation may participate in the strand discrimination of mismatch repair during eukaryotic DNA replication is discussed.

Original language	English
Pages (from-to)	7898-7902
Number of pages	5
Journal	Oncogene
Volume	23
Issue number	47
DOIs	https://doi.org/10.1038/sj.onc.1208111
Publication status	Published - Oct 14 2004
Externally published	Yes

Keywords

Dnmt1
Microsatellite instability
Mismatch repair

ASJC Scopus subject areas

Molecular Biology
Genetics
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/sj.onc.1208111

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title = "DNA methyltransferase Dnmt1 and mismatch repair",

abstract = "We have examined the relationship between DNA mismatch repair and deficiency of DNA methylation in a mouse embryonic cell line, Dnmt1 -/- ES, with homologous deletion of the gene coding for the maintenance DNA methyltransferase Dnmt1. With the use of a sensitive PCR for the assay of two mononucleotide- and three dinucleotide microsatellite markers that exhibited instabilities in mismatch repair-deficient cells, significantly higher frequencies of instability were detected at three of the five markers in Dnmt1-/- ES than the wild-type ES. The data suggest that Dnmt1 enzyme plays an integrating role in DNA replication and/or repair. The implication that Dnmt1 enzyme and/or cytosine methylation may participate in the strand discrimination of mismatch repair during eukaryotic DNA replication is discussed.",

keywords = "Dnmt1, Microsatellite instability, Mismatch repair",

author = "Wang, {Keh Yang} and Shen, {C. K.James}",

note = "Funding Information: We are indebted to Drs Y-S Jou and Peter Tsai at the National Health Research Institute, Taipei, Taiwan for their help on the genotyping experiments. We also thank Drs En Li and Paul Modrich for their critical and helpful comments. Dnmt1−/− ES cell line is a kind gift from Dr En Li. This work was supported by the National Science Council and the Academia Sinica, Taipei, Taiwan, ROC. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

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AU - Shen, C. K.James

N1 - Funding Information: We are indebted to Drs Y-S Jou and Peter Tsai at the National Health Research Institute, Taipei, Taiwan for their help on the genotyping experiments. We also thank Drs En Li and Paul Modrich for their critical and helpful comments. Dnmt1−/− ES cell line is a kind gift from Dr En Li. This work was supported by the National Science Council and the Academia Sinica, Taipei, Taiwan, ROC. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2004/10/14

Y1 - 2004/10/14

N2 - We have examined the relationship between DNA mismatch repair and deficiency of DNA methylation in a mouse embryonic cell line, Dnmt1 -/- ES, with homologous deletion of the gene coding for the maintenance DNA methyltransferase Dnmt1. With the use of a sensitive PCR for the assay of two mononucleotide- and three dinucleotide microsatellite markers that exhibited instabilities in mismatch repair-deficient cells, significantly higher frequencies of instability were detected at three of the five markers in Dnmt1-/- ES than the wild-type ES. The data suggest that Dnmt1 enzyme plays an integrating role in DNA replication and/or repair. The implication that Dnmt1 enzyme and/or cytosine methylation may participate in the strand discrimination of mismatch repair during eukaryotic DNA replication is discussed.

AB - We have examined the relationship between DNA mismatch repair and deficiency of DNA methylation in a mouse embryonic cell line, Dnmt1 -/- ES, with homologous deletion of the gene coding for the maintenance DNA methyltransferase Dnmt1. With the use of a sensitive PCR for the assay of two mononucleotide- and three dinucleotide microsatellite markers that exhibited instabilities in mismatch repair-deficient cells, significantly higher frequencies of instability were detected at three of the five markers in Dnmt1-/- ES than the wild-type ES. The data suggest that Dnmt1 enzyme plays an integrating role in DNA replication and/or repair. The implication that Dnmt1 enzyme and/or cytosine methylation may participate in the strand discrimination of mismatch repair during eukaryotic DNA replication is discussed.

KW - Dnmt1

KW - Microsatellite instability

KW - Mismatch repair

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ER -

DNA methyltransferase Dnmt1 and mismatch repair

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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