DNA looping alters local DNA conformation during transcription

Hai Young Wu, Leroy F. Liu

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


The effect of protein-mediated DNA looping on local DNA conformation during active transcription was studied using the lac repressor-operator system. Our results suggest that lac repressor-mediated DNA looping within a plasmid DNA molecule containing two lac repressor binding sequences in vivo effectively separates plasmid DNA into two topological domains. Supercoils generated by transcription within each topological domain can be rapidly removed by DNA topoisomerase I.

Original languageEnglish
Pages (from-to)615-622
Number of pages8
JournalJournal of Molecular Biology
Issue number4
Publication statusPublished - Jun 20 1991
Externally publishedYes


  • DNA looping
  • DNA supercoiling
  • DNA topoisomerases
  • RNA transcription
  • lac repressor

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


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