DNA looping alters local DNA conformation during transcription

Hai Young Wu; Leroy F. Liu

doi:10.1016/0022-2836(91)90658-S

DNA looping alters local DNA conformation during transcription

Hai Young Wu, Leroy F. Liu

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The effect of protein-mediated DNA looping on local DNA conformation during active transcription was studied using the lac repressor-operator system. Our results suggest that lac repressor-mediated DNA looping within a plasmid DNA molecule containing two lac repressor binding sequences in vivo effectively separates plasmid DNA into two topological domains. Supercoils generated by transcription within each topological domain can be rapidly removed by DNA topoisomerase I.

Original language	English
Pages (from-to)	615-622
Number of pages	8
Journal	Journal of Molecular Biology
Volume	219
Issue number	4
DOIs	https://doi.org/10.1016/0022-2836(91)90658-S
Publication status	Published - Jun 20 1991
Externally published	Yes

Keywords

DNA looping
DNA supercoiling
DNA topoisomerases
RNA transcription
lac repressor

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1016/0022-2836(91)90658-S

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abstract = "The effect of protein-mediated DNA looping on local DNA conformation during active transcription was studied using the lac repressor-operator system. Our results suggest that lac repressor-mediated DNA looping within a plasmid DNA molecule containing two lac repressor binding sequences in vivo effectively separates plasmid DNA into two topological domains. Supercoils generated by transcription within each topological domain can be rapidly removed by DNA topoisomerase I.",

keywords = "DNA looping, DNA supercoiling, DNA topoisomerases, RNA transcription, lac repressor",

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AU - Liu, Leroy F.

PY - 1991/6/20

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AB - The effect of protein-mediated DNA looping on local DNA conformation during active transcription was studied using the lac repressor-operator system. Our results suggest that lac repressor-mediated DNA looping within a plasmid DNA molecule containing two lac repressor binding sequences in vivo effectively separates plasmid DNA into two topological domains. Supercoils generated by transcription within each topological domain can be rapidly removed by DNA topoisomerase I.

KW - DNA looping

KW - DNA supercoiling

KW - DNA topoisomerases

KW - RNA transcription

KW - lac repressor

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JF - Journal of Molecular Biology

IS - 4

ER -

DNA looping alters local DNA conformation during transcription

Abstract

Keywords

ASJC Scopus subject areas

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