DNA aptamers as potential anti-HIV agents

Shan Ho Chou; Ko Hsin Chin; Andrew H.J. Wang

doi:10.1016/j.tibs.2005.03.004

DNA aptamers as potential anti-HIV agents

Shan Ho Chou, Ko Hsin Chin, Andrew H.J. Wang

Research output: Contribution to journal › Short survey › peer-review

64 Citations (Scopus)

Abstract

Guanine (G)-rich DNA sequences can adopt stable G-quadruplex structures by G-tetrad hydrogen-bonding and hydrophobic stacking. Recently, it has been shown that a DNA sequence forms an aptamer (termed 93del) and adopts a novel dimeric quadruplex folding topology in K⁺ solution. This aptamer exhibits anti-HIV1 integrase activity in the nanomolar range in vitro. A docking-based model of the 93del-integrase complex positions the DNA aptamer within a channel of the tetrameric integrase. This mutual fitting blocks several catalytic amino acid residues that are essential for integrase function, and accounts for the anti-HIV1 activity of the 93del aptamer.

Original language	English
Pages (from-to)	231-234
Number of pages	4
Journal	Trends in Biochemical Sciences
Volume	30
Issue number	5
DOIs	https://doi.org/10.1016/j.tibs.2005.03.004
Publication status	Published - May 2005
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.tibs.2005.03.004

Cite this

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title = "DNA aptamers as potential anti-HIV agents",

abstract = "Guanine (G)-rich DNA sequences can adopt stable G-quadruplex structures by G-tetrad hydrogen-bonding and hydrophobic stacking. Recently, it has been shown that a DNA sequence forms an aptamer (termed 93del) and adopts a novel dimeric quadruplex folding topology in K+ solution. This aptamer exhibits anti-HIV1 integrase activity in the nanomolar range in vitro. A docking-based model of the 93del-integrase complex positions the DNA aptamer within a channel of the tetrameric integrase. This mutual fitting blocks several catalytic amino acid residues that are essential for integrase function, and accounts for the anti-HIV1 activity of the 93del aptamer.",

author = "Chou, {Shan Ho} and Chin, {Ko Hsin} and Wang, {Andrew H.J.}",

note = "Funding Information: S-H.C. is grateful for support from the Ministry of Education and National Science Council (Taiwan, Republic of China).",

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language = "English",

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T1 - DNA aptamers as potential anti-HIV agents

AU - Chou, Shan Ho

AU - Chin, Ko Hsin

AU - Wang, Andrew H.J.

N1 - Funding Information: S-H.C. is grateful for support from the Ministry of Education and National Science Council (Taiwan, Republic of China).

PY - 2005/5

Y1 - 2005/5

N2 - Guanine (G)-rich DNA sequences can adopt stable G-quadruplex structures by G-tetrad hydrogen-bonding and hydrophobic stacking. Recently, it has been shown that a DNA sequence forms an aptamer (termed 93del) and adopts a novel dimeric quadruplex folding topology in K+ solution. This aptamer exhibits anti-HIV1 integrase activity in the nanomolar range in vitro. A docking-based model of the 93del-integrase complex positions the DNA aptamer within a channel of the tetrameric integrase. This mutual fitting blocks several catalytic amino acid residues that are essential for integrase function, and accounts for the anti-HIV1 activity of the 93del aptamer.

AB - Guanine (G)-rich DNA sequences can adopt stable G-quadruplex structures by G-tetrad hydrogen-bonding and hydrophobic stacking. Recently, it has been shown that a DNA sequence forms an aptamer (termed 93del) and adopts a novel dimeric quadruplex folding topology in K+ solution. This aptamer exhibits anti-HIV1 integrase activity in the nanomolar range in vitro. A docking-based model of the 93del-integrase complex positions the DNA aptamer within a channel of the tetrameric integrase. This mutual fitting blocks several catalytic amino acid residues that are essential for integrase function, and accounts for the anti-HIV1 activity of the 93del aptamer.

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DO - 10.1016/j.tibs.2005.03.004

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JO - Trends in Biochemical Sciences

JF - Trends in Biochemical Sciences

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DNA aptamers as potential anti-HIV agents

Abstract

ASJC Scopus subject areas

UN SDGs

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