Distinctive electrophysiological characteristics of right ventricular out-flow tract cardiomyocytes

Yen Yu Lu, Fa Po Chung, Yao Chang Chen, Chin Feng Tsai, Yu Hsun Kao, Tze Fan Chao, Jen Hung Huang, Shih Ann Chen, Yi Jen Chen

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Ventricular arrhythmias commonly originate from the right ventricular out-flow tract (RVOT). However, the electrophysiological characteristics and Ca2++ homoeostasis of RVOT cardiomyocytes remain unclear. Whole-cell patch clamp and indo-1 fluorometric ratio techniques were used to investigate action potentials, Ca2++ homoeostasis and ionic currents in isolated cardiomyocytes from the rabbit RVOT and right ventricular apex (RVA). Conventional microelectrodes were used to record the electrical activity before and after (KN-93, a Ca2++/calmodulin-dependent kinase II inhibitor, or ranolazine, a late sodium current inhibitor) treatment in RVOT and RVA tissue preparations under electrical pacing and ouabain (Na+/K+ ATPase inhibitor) administration. In contrast to RVA cardiomyocytes, RVOT cardiomyocytes were characterized by longer action potential duration measured at 90% and 50% repolarization, larger Ca2++ transients, higher Ca2++ stores, higher late Na+ and transient outward K+ currents, but smaller delayed rectifier K+, L-type Ca2++ currents and Na+-Ca2++ exchanger currents. RVOT cardiomyocytes showed significantly more pacing-induced delayed afterdepolarizations (22% versus 0%, P < 0.05) and ouabain-induced ventricular arrhythmias (94% versus 61%, P < 0.05) than RVA cardiomyocytes. Consistently, it took longer time (9 ± 1 versus 4 ± 1 min., P < 0.05) to eliminate ouabain-induced ventricular arrhythmias after application of KN-93 (but not ranolazine) in the RVOT in comparison with the RVA. These results indicate that RVOT cardiomyocytes have distinct electrophysiological characteristics with longer AP duration and greater Ca2++ content, which could contribute to the high RVOT arrhythmogenic activity.

Original languageEnglish
Pages (from-to)1540-1548
Number of pages9
JournalJournal of Cellular and Molecular Medicine
Issue number8
Publication statusPublished - 2014


  • Arrhythmogenicity
  • Calcium handling
  • Right ventricular out-flow tract
  • Ventricular arrhythmias

ASJC Scopus subject areas

  • Molecular Medicine
  • Cell Biology


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