Abstract

Target antigens are crucial for developing chimeric antigen receptor (CAR)-T cells, but their application to ovarian cancers is limited. This study aimed to identify potential genes as CAR-T-cell antigen candidates for ovarian cancers. A differential gene expression analysis was performed on ovarian cancer samples from four datasets obtained from the GEO datasets. Functional annotation, pathway analysis, protein localization, and gene expression analysis were conducted using various datasets and tools. An oncogenicity analysis and network analysis were also performed. In total, 153 differentially expressed genes were identified in ovarian cancer samples, with 60 differentially expressed genes expressing plasma membrane proteins suitable for CART-cell antigens. Among them, 21 plasma membrane proteins were predicted to be oncogenes in ovarian cancers, with nine proteins playing crucial roles in the network. Key genes identified in the oncogenic pathways of ovarian cancers included MUC1, CXCR4, EPCAM, RACGAP1, UBE2C, PRAME, SORT1, JUP, and CLDN3, suggesting them as recommended antigens for CAR-T-cell therapy for ovarian cancers. This study sheds light on potential targets for immunotherapy in ovarian cancers.

Original languageEnglish
Pages (from-to)11409-11433
Number of pages25
JournalAging
Volume16
Issue number14
DOIs
Publication statusPublished - Jul 18 2024

Keywords

  • CAR-T-cell antigen
  • chimeric antigen receptor (CAR)-T cell
  • differentially expressed gene (DEG)
  • ovarian cancer
  • protein-protein interaction (PPI) network

ASJC Scopus subject areas

  • Ageing
  • Cell Biology

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