Direct coating of culture medium from cells secreting classical swine fever virus E2 antigen on ELISA plates for detection of E2-specific antibodies

Ta Chun Cheng; Chu Hsiang Pan; Chien Shu Chen; Kuo Hsiang Chuang; Chih Hung Chuang; Chien Chaio Huang; Yu Yi Chu; Ya Chun Yang; Pei Yu Chu; Chien Han Kao; Yuan Chin Hsieh; Tian Lu Cheng

doi:10.1016/j.tvjl.2015.02.007

Direct coating of culture medium from cells secreting classical swine fever virus E2 antigen on ELISA plates for detection of E2-specific antibodies

Ta Chun Cheng
, Chu Hsiang Pan
, Chien Shu Chen
, Kuo Hsiang Chuang
, Chih Hung Chuang
, Chien Chaio Huang
, Yu Yi Chu
, Ya Chun Yang
, Pei Yu Chu
, Chien Han Kao
, Yuan Chin Hsieh
, Tian Lu Cheng

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The envelope glycoprotein E2 of classical swine fever virus (CSFV) is widely used as a marker for measuring vaccine efficacy and antibody titer. The glycosylation profile of E2 may affect the immunogenicity of the vaccine and the timing of re-vaccination. In this study, a human embryonic kidney cell line was used to secrete fully-glycosylated CSFV E2, which was then coated onto ELISA plates without purification or adjustment.The resulting E2-secreting medium-direct-coating (E2-mDc) ELISA was successfully used to measure anti-E2 antibody titers in vaccinated and field pig sera samples. Compared with a virus neutralization test (as standard), the E2-mDc ELISA was found to be more accurate (90%) than a commercial CSFV antibody diagnostic kit (62%). In conclusion, the mammalian cell-secreted antigen can provide cheap, accurate and effective assays for vaccine efficacy and disease diagnoses.

Original language	English
Pages (from-to)	107-109
Number of pages	3
Journal	Veterinary Journal
Volume	205
Issue number	1
DOIs	https://doi.org/10.1016/j.tvjl.2015.02.007
Publication status	Published - Jul 1 2015

Keywords

Classical swine fever virus
E2
Glycosylation
Mammalian expression
Medium-direct-coating ELISA

ASJC Scopus subject areas

Animal Science and Zoology
General Veterinary

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10.1016/j.tvjl.2015.02.007

Cite this

Cheng, T. C., Pan, C. H., Chen, C. S., Chuang, K. H., Chuang, C. H., Huang, C. C., Chu, Y. Y., Yang, Y. C., Chu, P. Y., Kao, C. H., Hsieh, Y. C., & Cheng, T. L. (2015). Direct coating of culture medium from cells secreting classical swine fever virus E2 antigen on ELISA plates for detection of E2-specific antibodies. Veterinary Journal, 205(1), 107-109. https://doi.org/10.1016/j.tvjl.2015.02.007

Cheng, TC, Pan, CH, Chen, CS, Chuang, KH, Chuang, CH, Huang, CC, Chu, YY, Yang, YC, Chu, PY, Kao, CH, Hsieh, YC & Cheng, TL 2015, 'Direct coating of culture medium from cells secreting classical swine fever virus E2 antigen on ELISA plates for detection of E2-specific antibodies', Veterinary Journal, vol. 205, no. 1, pp. 107-109. https://doi.org/10.1016/j.tvjl.2015.02.007

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title = "Direct coating of culture medium from cells secreting classical swine fever virus E2 antigen on ELISA plates for detection of E2-specific antibodies",

abstract = "The envelope glycoprotein E2 of classical swine fever virus (CSFV) is widely used as a marker for measuring vaccine efficacy and antibody titer. The glycosylation profile of E2 may affect the immunogenicity of the vaccine and the timing of re-vaccination. In this study, a human embryonic kidney cell line was used to secrete fully-glycosylated CSFV E2, which was then coated onto ELISA plates without purification or adjustment.The resulting E2-secreting medium-direct-coating (E2-mDc) ELISA was successfully used to measure anti-E2 antibody titers in vaccinated and field pig sera samples. Compared with a virus neutralization test (as standard), the E2-mDc ELISA was found to be more accurate (90\%) than a commercial CSFV antibody diagnostic kit (62\%). In conclusion, the mammalian cell-secreted antigen can provide cheap, accurate and effective assays for vaccine efficacy and disease diagnoses.",

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AU - Cheng, Ta Chun

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AU - Chuang, Kuo Hsiang

AU - Chuang, Chih Hung

AU - Huang, Chien Chaio

AU - Chu, Yu Yi

AU - Yang, Ya Chun

AU - Chu, Pei Yu

AU - Kao, Chien Han

AU - Hsieh, Yuan Chin

AU - Cheng, Tian Lu

PY - 2015/7/1

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AB - The envelope glycoprotein E2 of classical swine fever virus (CSFV) is widely used as a marker for measuring vaccine efficacy and antibody titer. The glycosylation profile of E2 may affect the immunogenicity of the vaccine and the timing of re-vaccination. In this study, a human embryonic kidney cell line was used to secrete fully-glycosylated CSFV E2, which was then coated onto ELISA plates without purification or adjustment.The resulting E2-secreting medium-direct-coating (E2-mDc) ELISA was successfully used to measure anti-E2 antibody titers in vaccinated and field pig sera samples. Compared with a virus neutralization test (as standard), the E2-mDc ELISA was found to be more accurate (90%) than a commercial CSFV antibody diagnostic kit (62%). In conclusion, the mammalian cell-secreted antigen can provide cheap, accurate and effective assays for vaccine efficacy and disease diagnoses.

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KW - Glycosylation

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KW - Medium-direct-coating ELISA

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Direct coating of culture medium from cells secreting classical swine fever virus E2 antigen on ELISA plates for detection of E2-specific antibodies

Abstract

Keywords

ASJC Scopus subject areas

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