Direct and selective metal-free N6-arylation of adenosine residues for simple fluorescence labeling of DNA and RNA

Guralamatta Siddappa Ravi Kumara; Anup Pandith; Young Jun Seo

doi:10.1039/d1cc02069b

Direct and selective metal-free N⁶-arylation of adenosine residues for simple fluorescence labeling of DNA and RNA

Guralamatta Siddappa Ravi Kumara, Anup Pandith, Young Jun Seo

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We have developed an unprecedented transition metal-free approach for the direct fluorescence turn-on labeling of natural oligonucleotides through selective N⁶-arylation of adenosine moieties. This method allows the simple and direct fluorescence labeling of natural unmodified DNA and RNA, but is dependent on the secondary structure, favoring single-stranded structures.

Original language	English
Pages (from-to)	5450-5453
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	44
DOIs	https://doi.org/10.1039/d1cc02069b
Publication status	Published - Jun 4 2021
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/d1cc02069b

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title = "Direct and selective metal-free N6-arylation of adenosine residues for simple fluorescence labeling of DNA and RNA",

abstract = "We have developed an unprecedented transition metal-free approach for the direct fluorescence turn-on labeling of natural oligonucleotides through selective N6-arylation of adenosine moieties. This method allows the simple and direct fluorescence labeling of natural unmodified DNA and RNA, but is dependent on the secondary structure, favoring single-stranded structures.",

author = "{Ravi Kumara}, {Guralamatta Siddappa} and Anup Pandith and Seo, {Young Jun}",

note = "Funding Information: We thank Basic Science Research Program for supporting this study through the National Research Foundation of Korea (NRF) (2021R1A2C1003804). Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021.",

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AU - Ravi Kumara, Guralamatta Siddappa

AU - Pandith, Anup

AU - Seo, Young Jun

N1 - Funding Information: We thank Basic Science Research Program for supporting this study through the National Research Foundation of Korea (NRF) (2021R1A2C1003804). Publisher Copyright: © The Royal Society of Chemistry 2021.

PY - 2021/6/4

Y1 - 2021/6/4

N2 - We have developed an unprecedented transition metal-free approach for the direct fluorescence turn-on labeling of natural oligonucleotides through selective N6-arylation of adenosine moieties. This method allows the simple and direct fluorescence labeling of natural unmodified DNA and RNA, but is dependent on the secondary structure, favoring single-stranded structures.

AB - We have developed an unprecedented transition metal-free approach for the direct fluorescence turn-on labeling of natural oligonucleotides through selective N6-arylation of adenosine moieties. This method allows the simple and direct fluorescence labeling of natural unmodified DNA and RNA, but is dependent on the secondary structure, favoring single-stranded structures.

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DO - 10.1039/d1cc02069b

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JO - Chemical Communications

JF - Chemical Communications

IS - 44

ER -

Direct and selective metal-free N⁶-arylation of adenosine residues for simple fluorescence labeling of DNA and RNA

Abstract

ASJC Scopus subject areas

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