Dioscorea nipponica Makino inhibits migration and invasion of human oral cancer HSC-3 cells by transcriptional inhibition of matrix metalloproteinase-2 through modulation of CREB and AP-1 activity

Ming Hsien Chien; Tsung Ho Ying; Yih Shou Hsieh; Yu Chao Chang; Chia Ming Yeh; Jiunn Liang Ko; Wen Sen Lee; Jer Hua Chang; Shun Fa Yang

doi:10.1016/j.fct.2011.12.016

Dioscorea nipponica Makino inhibits migration and invasion of human oral cancer HSC-3 cells by transcriptional inhibition of matrix metalloproteinase-2 through modulation of CREB and AP-1 activity

Ming Hsien Chien, Tsung Ho Ying, Yih Shou Hsieh, Yu Chao Chang, Chia Ming Yeh, Jiunn Liang Ko, Wen Sen Lee, Jer Hua Chang, Shun Fa Yang

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

Oral cancer mortality has increased during the last decade due to the difficulties in treating related metastasis. Dioscorea nipponica Makino, a popular folk medicine, exerts anti-obesity and anti-inflammation properties. However, the effect of this folk medicine on metastasis of oral cancer has yet to be fully elucidated. The present study demonstrates that D. nipponica extracts (DNE), at a range of concentrations (0-50 μg/mL), concentration-dependently inhibited migration/invasion capacities of human oral cancer cells, HSC-3, without cytotoxic effects. The anti-migration effect of DNE was also observed in two other OSCC cell lines, Ca9-22 and Cal-27. Zymography, real time PCR, and Western blotting analyses revealed that DNE inhibited matrix metalloproteinase-2 (MMP-2) enzyme activity, and RNA and protein expression. The inhibitory effects of DNE on MMP-2 proceeded by up-regulating tissue inhibitor of metalloproteinase-2 (TIMP-2), as well as suppressing nuclear translocation and DNA binding activity of cAMP response element-binding (CREB) and activating protein-1 (AP-1) on the MMP-2 promoter in HSC-3 cells. In conclusion, DNE inhibited the invasion of oral cancer cells and may have potential use as a chemopreventive agent against oral cancer metastasis.

Original language	English
Pages (from-to)	558-566
Number of pages	9
Journal	Food and Chemical Toxicology
Volume	50
Issue number	3-4
DOIs	https://doi.org/10.1016/j.fct.2011.12.016
Publication status	Published - Mar 2012

Keywords

Dioscorea nipponica
Invasion
MMP-2
Migration
Oral cancer

ASJC Scopus subject areas

Food Science
Toxicology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.fct.2011.12.016

Cite this

Chien, M. H., Ying, T. H., Hsieh, Y. S., Chang, Y. C., Yeh, C. M., Ko, J. L., Lee, W. S., Chang, J. H., & Yang, S. F. (2012). Dioscorea nipponica Makino inhibits migration and invasion of human oral cancer HSC-3 cells by transcriptional inhibition of matrix metalloproteinase-2 through modulation of CREB and AP-1 activity. Food and Chemical Toxicology, 50(3-4), 558-566. https://doi.org/10.1016/j.fct.2011.12.016

Dioscorea nipponica Makino inhibits migration and invasion of human oral cancer HSC-3 cells by transcriptional inhibition of matrix metalloproteinase-2 through modulation of CREB and AP-1 activity. / Chien, Ming Hsien; Ying, Tsung Ho; Hsieh, Yih Shou et al.
In: Food and Chemical Toxicology, Vol. 50, No. 3-4, 03.2012, p. 558-566.

Research output: Contribution to journal › Article › peer-review

Chien, MH, Ying, TH, Hsieh, YS, Chang, YC, Yeh, CM, Ko, JL, Lee, WS, Chang, JH & Yang, SF 2012, 'Dioscorea nipponica Makino inhibits migration and invasion of human oral cancer HSC-3 cells by transcriptional inhibition of matrix metalloproteinase-2 through modulation of CREB and AP-1 activity', Food and Chemical Toxicology, vol. 50, no. 3-4, pp. 558-566. https://doi.org/10.1016/j.fct.2011.12.016

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title = "Dioscorea nipponica Makino inhibits migration and invasion of human oral cancer HSC-3 cells by transcriptional inhibition of matrix metalloproteinase-2 through modulation of CREB and AP-1 activity",

abstract = "Oral cancer mortality has increased during the last decade due to the difficulties in treating related metastasis. Dioscorea nipponica Makino, a popular folk medicine, exerts anti-obesity and anti-inflammation properties. However, the effect of this folk medicine on metastasis of oral cancer has yet to be fully elucidated. The present study demonstrates that D. nipponica extracts (DNE), at a range of concentrations (0-50 μg/mL), concentration-dependently inhibited migration/invasion capacities of human oral cancer cells, HSC-3, without cytotoxic effects. The anti-migration effect of DNE was also observed in two other OSCC cell lines, Ca9-22 and Cal-27. Zymography, real time PCR, and Western blotting analyses revealed that DNE inhibited matrix metalloproteinase-2 (MMP-2) enzyme activity, and RNA and protein expression. The inhibitory effects of DNE on MMP-2 proceeded by up-regulating tissue inhibitor of metalloproteinase-2 (TIMP-2), as well as suppressing nuclear translocation and DNA binding activity of cAMP response element-binding (CREB) and activating protein-1 (AP-1) on the MMP-2 promoter in HSC-3 cells. In conclusion, DNE inhibited the invasion of oral cancer cells and may have potential use as a chemopreventive agent against oral cancer metastasis.",

keywords = "Dioscorea nipponica, Invasion, MMP-2, Migration, Oral cancer",

author = "Chien, {Ming Hsien} and Ying, {Tsung Ho} and Hsieh, {Yih Shou} and Chang, {Yu Chao} and Yeh, {Chia Ming} and Ko, {Jiunn Liang} and Lee, {Wen Sen} and Chang, {Jer Hua} and Yang, {Shun Fa}",

note = "Funding Information: This study was supported by Grants from Wan Fang Hospital-Taipei Medical University (Grant Nos. 100swf04 to Dr. Chien and NSC-100-2636-B-040-001-MY3 to Dr. Yang). ",

year = "2012",

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doi = "10.1016/j.fct.2011.12.016",

language = "English",

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T1 - Dioscorea nipponica Makino inhibits migration and invasion of human oral cancer HSC-3 cells by transcriptional inhibition of matrix metalloproteinase-2 through modulation of CREB and AP-1 activity

AU - Chien, Ming Hsien

AU - Ying, Tsung Ho

AU - Hsieh, Yih Shou

AU - Chang, Yu Chao

AU - Yeh, Chia Ming

AU - Ko, Jiunn Liang

AU - Lee, Wen Sen

AU - Chang, Jer Hua

AU - Yang, Shun Fa

N1 - Funding Information: This study was supported by Grants from Wan Fang Hospital-Taipei Medical University (Grant Nos. 100swf04 to Dr. Chien and NSC-100-2636-B-040-001-MY3 to Dr. Yang).

PY - 2012/3

Y1 - 2012/3

N2 - Oral cancer mortality has increased during the last decade due to the difficulties in treating related metastasis. Dioscorea nipponica Makino, a popular folk medicine, exerts anti-obesity and anti-inflammation properties. However, the effect of this folk medicine on metastasis of oral cancer has yet to be fully elucidated. The present study demonstrates that D. nipponica extracts (DNE), at a range of concentrations (0-50 μg/mL), concentration-dependently inhibited migration/invasion capacities of human oral cancer cells, HSC-3, without cytotoxic effects. The anti-migration effect of DNE was also observed in two other OSCC cell lines, Ca9-22 and Cal-27. Zymography, real time PCR, and Western blotting analyses revealed that DNE inhibited matrix metalloproteinase-2 (MMP-2) enzyme activity, and RNA and protein expression. The inhibitory effects of DNE on MMP-2 proceeded by up-regulating tissue inhibitor of metalloproteinase-2 (TIMP-2), as well as suppressing nuclear translocation and DNA binding activity of cAMP response element-binding (CREB) and activating protein-1 (AP-1) on the MMP-2 promoter in HSC-3 cells. In conclusion, DNE inhibited the invasion of oral cancer cells and may have potential use as a chemopreventive agent against oral cancer metastasis.

AB - Oral cancer mortality has increased during the last decade due to the difficulties in treating related metastasis. Dioscorea nipponica Makino, a popular folk medicine, exerts anti-obesity and anti-inflammation properties. However, the effect of this folk medicine on metastasis of oral cancer has yet to be fully elucidated. The present study demonstrates that D. nipponica extracts (DNE), at a range of concentrations (0-50 μg/mL), concentration-dependently inhibited migration/invasion capacities of human oral cancer cells, HSC-3, without cytotoxic effects. The anti-migration effect of DNE was also observed in two other OSCC cell lines, Ca9-22 and Cal-27. Zymography, real time PCR, and Western blotting analyses revealed that DNE inhibited matrix metalloproteinase-2 (MMP-2) enzyme activity, and RNA and protein expression. The inhibitory effects of DNE on MMP-2 proceeded by up-regulating tissue inhibitor of metalloproteinase-2 (TIMP-2), as well as suppressing nuclear translocation and DNA binding activity of cAMP response element-binding (CREB) and activating protein-1 (AP-1) on the MMP-2 promoter in HSC-3 cells. In conclusion, DNE inhibited the invasion of oral cancer cells and may have potential use as a chemopreventive agent against oral cancer metastasis.

KW - Dioscorea nipponica

KW - Invasion

KW - MMP-2

KW - Migration

KW - Oral cancer

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Dioscorea nipponica Makino inhibits migration and invasion of human oral cancer HSC-3 cells by transcriptional inhibition of matrix metalloproteinase-2 through modulation of CREB and AP-1 activity

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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