TY - JOUR
T1 - Dietary supplementation of rutin and rutin-rich buckwheat elevates endogenous glucagon-like peptide 1 levels to facilitate glycemic control in type 2 diabetic mice
AU - Lee, Lin Chien
AU - Hou, Yu Chang
AU - Hsieh, Ying Ying
AU - Chen, Yu Hsin
AU - Shen, Yuh Chiang
AU - Lee, I. Jung
AU - Monica Shih, Meng Chun
AU - Hou, Wen Chi
AU - Liu, Hui Kang
N1 - Funding Information:
Tartary buckwheat Taichung No. 2 (TBW) was provided by the Taichung District Agricultural Research and Extension Station (TDAIS), Council of Agriculture, Executive Yuan, Taiwan. TBW seeds were manually planted at the TDAIS farm in mid-March. The flowering period occurred 6 weeks after seeding. After this time, whole plants were harvested each week, and growth conditions were recorded. Harvested materials were dried at 40 °C prior to being crushed for chemical profiling. TBW seeds were purchased from local farmers in Taichung, and the hull and grout were separated and crushed through 70 mesh to facilitate subsequent extraction.
Funding Information:
We thank the National Laboratory Animal Center (NLAC), NARLabs, Taiwan, for technical support in mouse breeding. This research was supported by grants from the National Research Institute of Chinese Medicine (Grant No.: MOHW109-NRICM-M-315-000105), the Ministry of Science and Technology (Grant Nos.: MOST 108-2320-B-077-002 and MOST 109-2320-B-077-006), Cheng Hsin General Hospital [Grant No.: CHGH109-(N)27], Taoyuan General Hospital (Grant No.: PTH109014 and PTH110041 ), and the Taichung District Agriculture Research and Extension Station (Grant Nos.: 107B02 and 108B02).
Funding Information:
We thank the National Laboratory Animal Center (NLAC), NARLabs, Taiwan, for technical support in mouse breeding. This research was supported by grants from the National Research Institute of Chinese Medicine (Grant No.: MOHW109-NRICM-M-315-000105), the Ministry of Science and Technology (Grant Nos.: MOST 108-2320-B-077-002 and MOST 109-2320-B-077-006), Cheng Hsin General Hospital [Grant No.: CHGH109-(N)27], Taoyuan General Hospital (Grant No.: PTH109014 and PTH110041), and the Taichung District Agriculture Research and Extension Station (Grant Nos.: 107B02 and 108B02).
Publisher Copyright:
© 2021
PY - 2021/10
Y1 - 2021/10
N2 - Rutin and rutin-rich buckwheat are commonly used as alternative medicines due to their wide range of health benefits. The present study aims to investigate their anti-diabetes effects and the association with glucagon-like peptide 1 (GLP-1) levels. A murine model of type 2 diabetes (T2D), induced by a high-fat diet combined with streptozocin injection, was utilized. T2D mice were fed 3% buckwheat, 10% buckwheat, or 0.1% rutin as part of their diet for 4 weeks. Dipeptidyl peptidase-4 assays, secretin tumor cell line (STC-1) cells, and GLP-1 receptor-knockout mice were employed for mechanistic studies. Dietary consumption of 10% buckwheat and 0.1% rutin provided beneficial outcomes including lowering blood glucose levels and HbA1c, enhancing HOMA-B%, improving glucose tolerance and pyruvate tolerance. Serum GLP-1, ghrelin, leptin, and adiponectin levels were also increased at the end of the treatment. Increased GLP-1 levels and the improvement of β-cell function were closely associated with the blood glucose-lowering effects observed. Hypoglycemic effects of buckwheat and rutin supplement were abolished in diabetic GLP-1 receptor knockout mice. In conclusion, rutin and related herbal materials are expected to provide health benefits due to their ability to modulate endogenous GLP-1 levels in T2D mice.
AB - Rutin and rutin-rich buckwheat are commonly used as alternative medicines due to their wide range of health benefits. The present study aims to investigate their anti-diabetes effects and the association with glucagon-like peptide 1 (GLP-1) levels. A murine model of type 2 diabetes (T2D), induced by a high-fat diet combined with streptozocin injection, was utilized. T2D mice were fed 3% buckwheat, 10% buckwheat, or 0.1% rutin as part of their diet for 4 weeks. Dipeptidyl peptidase-4 assays, secretin tumor cell line (STC-1) cells, and GLP-1 receptor-knockout mice were employed for mechanistic studies. Dietary consumption of 10% buckwheat and 0.1% rutin provided beneficial outcomes including lowering blood glucose levels and HbA1c, enhancing HOMA-B%, improving glucose tolerance and pyruvate tolerance. Serum GLP-1, ghrelin, leptin, and adiponectin levels were also increased at the end of the treatment. Increased GLP-1 levels and the improvement of β-cell function were closely associated with the blood glucose-lowering effects observed. Hypoglycemic effects of buckwheat and rutin supplement were abolished in diabetic GLP-1 receptor knockout mice. In conclusion, rutin and related herbal materials are expected to provide health benefits due to their ability to modulate endogenous GLP-1 levels in T2D mice.
KW - Dipeptidyl peptidase-4
KW - GLP-1-receptor-knockout mice
KW - Glucagon-like peptide 1
KW - Rutin
KW - Tartary buckwheat
KW - Type 2 diabetes
UR - https://www.scopus.com/pages/publications/85111223059
UR - https://www.scopus.com/pages/publications/85111223059#tab=citedBy
U2 - 10.1016/j.jff.2021.104653
DO - 10.1016/j.jff.2021.104653
M3 - Article
AN - SCOPUS:85111223059
SN - 1756-4646
VL - 85
JO - Journal of Functional Foods
JF - Journal of Functional Foods
M1 - 104653
ER -
SDG 3 Good Health and Well-being