Diacylglycerol formation induced by human plasmin in cultured endothelial cells

Wen Chang Chang; Guey Yueh Shi; Ming T. Lin; Chauyin J. Jen; Lih Yuh C. Wing; Hua Lin Wu

doi:10.1016/0049-3848(94)90210-0

Diacylglycerol formation induced by human plasmin in cultured endothelial cells

Wen Chang Chang, Guey Yueh Shi, Ming T. Lin, Chauyin J. Jen, Lih Yuh C. Wing, Hua Lin Wu

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Treatment of cultured bovine carotid artery endothelial cells with 10^-7M plasmin increased the cellular diacylglycerol which was determined by the formation of [³H]palmitate-labeled diacylglycerol and diacylglycerol mass. Upon the stimulation with plasmin, a gradual increase in diacylglycerol formation was observed within 20 min then slightly declined. The maximal effect during the 1-h time course study was 45 and 55% increases in [³H]palmitate-labeled diacylgycerol and diacylglycerol mass, respectively, at 20 min after plasmin treatment. Formation of phosphatidylethanol was also studied in [³H]palmitate-prelabeled cells in the presence of ethanol. Treatment with plasmin for 20 min induced a significant 45% increase in phosphatidylethanol formation. The present results indicate that the plasmin-induced diacylglycerol formation in endothelial cells was at least in part mediated through the phospholipase D activation.

Original language	English
Pages (from-to)	89-95
Number of pages	7
Journal	Thrombosis Research
Volume	76
Issue number	1
DOIs	https://doi.org/10.1016/0049-3848(94)90210-0
Publication status	Published - Oct 1 1994
Externally published	Yes

Keywords

diacylglycerol
endothelial cells
plasmin

ASJC Scopus subject areas

Hematology

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10.1016/0049-3848(94)90210-0

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@article{3d1cbe6bf1c64a19ab839255aec4d2b4,

title = "Diacylglycerol formation induced by human plasmin in cultured endothelial cells",

abstract = "Treatment of cultured bovine carotid artery endothelial cells with 10-7M plasmin increased the cellular diacylglycerol which was determined by the formation of [3H]palmitate-labeled diacylglycerol and diacylglycerol mass. Upon the stimulation with plasmin, a gradual increase in diacylglycerol formation was observed within 20 min then slightly declined. The maximal effect during the 1-h time course study was 45 and 55% increases in [3H]palmitate-labeled diacylgycerol and diacylglycerol mass, respectively, at 20 min after plasmin treatment. Formation of phosphatidylethanol was also studied in [3H]palmitate-prelabeled cells in the presence of ethanol. Treatment with plasmin for 20 min induced a significant 45% increase in phosphatidylethanol formation. The present results indicate that the plasmin-induced diacylglycerol formation in endothelial cells was at least in part mediated through the phospholipase D activation.",

keywords = "diacylglycerol, endothelial cells, plasmin",

author = "Chang, {Wen Chang} and Shi, {Guey Yueh} and Lin, {Ming T.} and Jen, {Chauyin J.} and Wing, {Lih Yuh C.} and Wu, {Hua Lin}",

note = "Funding Information: This study was supported in part by Grants NSCXl-0420-B-006~548-MO9 and NSC81-0420-B-006-551-MO9 from the National Science Council, and b Grant DOH82-HRC06 from the Department of Health of the Republic of China. TK anks are due to I.P. Lee and Y.L. Chang for their technical assistance and to Y.J. Chang for her secretarial assistance.",

year = "1994",

month = oct,

day = "1",

doi = "10.1016/0049-3848(94)90210-0",

language = "English",

volume = "76",

pages = "89--95",

journal = "Thrombosis Research",

issn = "0049-3848",

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T1 - Diacylglycerol formation induced by human plasmin in cultured endothelial cells

AU - Chang, Wen Chang

AU - Shi, Guey Yueh

AU - Lin, Ming T.

AU - Jen, Chauyin J.

AU - Wing, Lih Yuh C.

AU - Wu, Hua Lin

N1 - Funding Information: This study was supported in part by Grants NSCXl-0420-B-006~548-MO9 and NSC81-0420-B-006-551-MO9 from the National Science Council, and b Grant DOH82-HRC06 from the Department of Health of the Republic of China. TK anks are due to I.P. Lee and Y.L. Chang for their technical assistance and to Y.J. Chang for her secretarial assistance.

PY - 1994/10/1

Y1 - 1994/10/1

N2 - Treatment of cultured bovine carotid artery endothelial cells with 10-7M plasmin increased the cellular diacylglycerol which was determined by the formation of [3H]palmitate-labeled diacylglycerol and diacylglycerol mass. Upon the stimulation with plasmin, a gradual increase in diacylglycerol formation was observed within 20 min then slightly declined. The maximal effect during the 1-h time course study was 45 and 55% increases in [3H]palmitate-labeled diacylgycerol and diacylglycerol mass, respectively, at 20 min after plasmin treatment. Formation of phosphatidylethanol was also studied in [3H]palmitate-prelabeled cells in the presence of ethanol. Treatment with plasmin for 20 min induced a significant 45% increase in phosphatidylethanol formation. The present results indicate that the plasmin-induced diacylglycerol formation in endothelial cells was at least in part mediated through the phospholipase D activation.

AB - Treatment of cultured bovine carotid artery endothelial cells with 10-7M plasmin increased the cellular diacylglycerol which was determined by the formation of [3H]palmitate-labeled diacylglycerol and diacylglycerol mass. Upon the stimulation with plasmin, a gradual increase in diacylglycerol formation was observed within 20 min then slightly declined. The maximal effect during the 1-h time course study was 45 and 55% increases in [3H]palmitate-labeled diacylgycerol and diacylglycerol mass, respectively, at 20 min after plasmin treatment. Formation of phosphatidylethanol was also studied in [3H]palmitate-prelabeled cells in the presence of ethanol. Treatment with plasmin for 20 min induced a significant 45% increase in phosphatidylethanol formation. The present results indicate that the plasmin-induced diacylglycerol formation in endothelial cells was at least in part mediated through the phospholipase D activation.

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KW - endothelial cells

KW - plasmin

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Diacylglycerol formation induced by human plasmin in cultured endothelial cells

Abstract

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