Development of vessel mimicking microfluidic device for studying mechano-response of endothelial cells

Pei Yu Chu; Han Yun Hsieh; Pei Shan Chung; Pai Wen Wang; Ming Chung Wu; Yin Quan Chen; Jean Cheng Kuo; Yu Jui Fan

doi:10.1016/j.isci.2023.106927

Development of vessel mimicking microfluidic device for studying mechano-response of endothelial cells

Pei Yu Chu, Han Yun Hsieh, Pei Shan Chung, Pai Wen Wang, Ming Chung Wu, Yin Quan Chen, Jean Cheng Kuo, Yu Jui Fan

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The objective of this study is to develop a device to mimic a microfluidic system of human arterial blood vessels. The device combines fluid shear stress (FSS) and cyclic stretch (CS), which are resulting from blood flow and blood pressure, respectively. The device can reveal real-time observation of dynamic morphological change of cells in different flow fields (continuous flow, reciprocating flow and pulsatile flow) and stretch. We observe the effects of FSS and CS on endothelial cells (ECs), including ECs align their cytoskeleton proteins with the fluid flow direction and paxillin redistribution to the cell periphery or the end of stress fibers. Thus, understanding the morphological and functional changes of endothelial cells on physical stimuli can help us to prevent and improve the treatment of cardiovascular diseases.

Original language	English
Article number	106927
Journal	iScience
Volume	26
Issue number	6
DOIs	https://doi.org/10.1016/j.isci.2023.106927
Publication status	Published - Jun 16 2023

Keywords

Biodevices
Bioengineering
Cardiovascular medicine

ASJC Scopus subject areas

General

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.isci.2023.106927

Cite this

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title = "Development of vessel mimicking microfluidic device for studying mechano-response of endothelial cells",

abstract = "The objective of this study is to develop a device to mimic a microfluidic system of human arterial blood vessels. The device combines fluid shear stress (FSS) and cyclic stretch (CS), which are resulting from blood flow and blood pressure, respectively. The device can reveal real-time observation of dynamic morphological change of cells in different flow fields (continuous flow, reciprocating flow and pulsatile flow) and stretch. We observe the effects of FSS and CS on endothelial cells (ECs), including ECs align their cytoskeleton proteins with the fluid flow direction and paxillin redistribution to the cell periphery or the end of stress fibers. Thus, understanding the morphological and functional changes of endothelial cells on physical stimuli can help us to prevent and improve the treatment of cardiovascular diseases.",

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doi = "10.1016/j.isci.2023.106927",

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AU - Chu, Pei Yu

AU - Hsieh, Han Yun

AU - Chung, Pei Shan

AU - Wang, Pai Wen

AU - Wu, Ming Chung

AU - Chen, Yin Quan

AU - Kuo, Jean Cheng

AU - Fan, Yu Jui

PY - 2023/6/16

Y1 - 2023/6/16

N2 - The objective of this study is to develop a device to mimic a microfluidic system of human arterial blood vessels. The device combines fluid shear stress (FSS) and cyclic stretch (CS), which are resulting from blood flow and blood pressure, respectively. The device can reveal real-time observation of dynamic morphological change of cells in different flow fields (continuous flow, reciprocating flow and pulsatile flow) and stretch. We observe the effects of FSS and CS on endothelial cells (ECs), including ECs align their cytoskeleton proteins with the fluid flow direction and paxillin redistribution to the cell periphery or the end of stress fibers. Thus, understanding the morphological and functional changes of endothelial cells on physical stimuli can help us to prevent and improve the treatment of cardiovascular diseases.

AB - The objective of this study is to develop a device to mimic a microfluidic system of human arterial blood vessels. The device combines fluid shear stress (FSS) and cyclic stretch (CS), which are resulting from blood flow and blood pressure, respectively. The device can reveal real-time observation of dynamic morphological change of cells in different flow fields (continuous flow, reciprocating flow and pulsatile flow) and stretch. We observe the effects of FSS and CS on endothelial cells (ECs), including ECs align their cytoskeleton proteins with the fluid flow direction and paxillin redistribution to the cell periphery or the end of stress fibers. Thus, understanding the morphological and functional changes of endothelial cells on physical stimuli can help us to prevent and improve the treatment of cardiovascular diseases.

KW - Biodevices

KW - Bioengineering

KW - Cardiovascular medicine

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Development of vessel mimicking microfluidic device for studying mechano-response of endothelial cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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