DEVELOPMENT OF MICROWELL ARRAY FOR CELL TRAPPING AND SHRNA DELIVERY USING ELECTROPORATION

Han Yun Hsieh, Che Yi Lee, Jian Chie Chao, Yu Jui Fan, Horn Jiunn Sheen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

First, we use microelectromechanical system (MEMS) technology to fabricate devices with different geometric structures on indium tin oxide (ITO) glass. The devices will be connected to an external electrical signal source to make the cells captured into the etched holes with negative dielectrophoresis (nDEP) to achieve cell manipulation. Second, we modulate the frequency of the electrical signal to achieve cell electroporation, and stain the cells with CFDA-SE and calcein to verify that after electropration, substances can be delivered from inside or outside the cell, and to find the optimal device for shRNA delivery.

Original languageEnglish
Title of host publicationMicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1231-1232
Number of pages2
ISBN (Electronic)9781733419048
Publication statusPublished - 2022
Event26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 - Hybrid, Hangzhou, China
Duration: Oct 23 2022Oct 27 2022

Publication series

NameMicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022
Country/TerritoryChina
CityHybrid, Hangzhou
Period10/23/2210/27/22

Keywords

  • Dielectrophoresis
  • Electroporation
  • ITO glass
  • MEMS
  • shRNA delivery

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering
  • General Chemistry
  • Control and Systems Engineering

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