Development of an RPLC-based method for measurement of indoprofen stability and validation of the method

Yi B. Liou; Fu A. Chen; Jen A. Lee; Hsiu O. Ho; An-Bang Wu

doi:10.1002/cjoc.200690294

Development of an RPLC-based method for measurement of indoprofen stability and validation of the method

Yi B. Liou, Fu A. Chen, Jen A. Lee, Hsiu O. Ho, An-Bang Wu

Research output: Contribution to journal › Article › peer-review

Abstract

A method for quantitative measurement of the photochemical decomposition of the anti-inflammatory agent, indoprofen (INP) is descriped. An RPLC-based assay that could determine the extent of degradation of INP in a rapid, sensitive, and accurate manner was developed. The method was validated under photoirradiation. Quantitation was monitored with an Inertsil ODS-3V column using a mobile phase of acetonitril and 1% HOAc solution in deionized H ₂O. Statistics relevant to the system criteria, peak integrity and resolution among the parent drug and its degradation products were performed. From the intra- and inter-day tests, the coefficients of variation were found to range from 0.59% to 4.25% for the former and from 0.71% to 4.86% for the latter. The good selectivity and specificity of this RPLC-based procedure render it suitable for measurements of INP stability.

Original language	English
Pages (from-to)	1570-1574
Number of pages	5
Journal	Chinese Journal of Chemistry
Volume	24
Issue number	11
DOIs	https://doi.org/10.1002/cjoc.200690294
Publication status	Published - Nov 2006

Keywords

Indoprofen
Photodegradation
Quantitation

ASJC Scopus subject areas

Chemistry(all)

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10.1002/cjoc.200690294

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title = "Development of an RPLC-based method for measurement of indoprofen stability and validation of the method",

abstract = "A method for quantitative measurement of the photochemical decomposition of the anti-inflammatory agent, indoprofen (INP) is descriped. An RPLC-based assay that could determine the extent of degradation of INP in a rapid, sensitive, and accurate manner was developed. The method was validated under photoirradiation. Quantitation was monitored with an Inertsil ODS-3V column using a mobile phase of acetonitril and 1% HOAc solution in deionized H 2O. Statistics relevant to the system criteria, peak integrity and resolution among the parent drug and its degradation products were performed. From the intra- and inter-day tests, the coefficients of variation were found to range from 0.59% to 4.25% for the former and from 0.71% to 4.86% for the latter. The good selectivity and specificity of this RPLC-based procedure render it suitable for measurements of INP stability.",

keywords = "Indoprofen, Photodegradation, Quantitation",

author = "Liou, {Yi B.} and Chen, {Fu A.} and Lee, {Jen A.} and Ho, {Hsiu O.} and An-Bang Wu",

year = "2006",

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doi = "10.1002/cjoc.200690294",

language = "English",

volume = "24",

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journal = "Chinese Journal of Chemistry",

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publisher = "Shanghai Institute of Organic Chemistry",

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T1 - Development of an RPLC-based method for measurement of indoprofen stability and validation of the method

AU - Liou, Yi B.

AU - Chen, Fu A.

AU - Lee, Jen A.

AU - Ho, Hsiu O.

AU - Wu, An-Bang

PY - 2006/11

Y1 - 2006/11

N2 - A method for quantitative measurement of the photochemical decomposition of the anti-inflammatory agent, indoprofen (INP) is descriped. An RPLC-based assay that could determine the extent of degradation of INP in a rapid, sensitive, and accurate manner was developed. The method was validated under photoirradiation. Quantitation was monitored with an Inertsil ODS-3V column using a mobile phase of acetonitril and 1% HOAc solution in deionized H 2O. Statistics relevant to the system criteria, peak integrity and resolution among the parent drug and its degradation products were performed. From the intra- and inter-day tests, the coefficients of variation were found to range from 0.59% to 4.25% for the former and from 0.71% to 4.86% for the latter. The good selectivity and specificity of this RPLC-based procedure render it suitable for measurements of INP stability.

AB - A method for quantitative measurement of the photochemical decomposition of the anti-inflammatory agent, indoprofen (INP) is descriped. An RPLC-based assay that could determine the extent of degradation of INP in a rapid, sensitive, and accurate manner was developed. The method was validated under photoirradiation. Quantitation was monitored with an Inertsil ODS-3V column using a mobile phase of acetonitril and 1% HOAc solution in deionized H 2O. Statistics relevant to the system criteria, peak integrity and resolution among the parent drug and its degradation products were performed. From the intra- and inter-day tests, the coefficients of variation were found to range from 0.59% to 4.25% for the former and from 0.71% to 4.86% for the latter. The good selectivity and specificity of this RPLC-based procedure render it suitable for measurements of INP stability.

KW - Indoprofen

KW - Photodegradation

KW - Quantitation

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DO - 10.1002/cjoc.200690294

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SP - 1570

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JO - Chinese Journal of Chemistry

JF - Chinese Journal of Chemistry

IS - 11

ER -

Development of an RPLC-based method for measurement of indoprofen stability and validation of the method

Abstract

Keywords

ASJC Scopus subject areas

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