TY - JOUR
T1 - Development of a parallel three-dimensional microfluidic device for high-throughput cytometry
AU - Fan, Yu Jui
AU - Hsiao, Yu Cheng
AU - Weng, Yen Ling
AU - Chen, Yi Hsiang
AU - Chiou, Pei Yu
AU - Sheen, Horn Jiunn
N1 - Funding Information:
This work was supported by the Ministry of Science and Technology of Taiwan under grant numbers MOST 109-2636-E-038-003, 108-2811-E-002-542, and 108-2221-E-002-174-MY3. We would also like to thank the NEMS Research Center, National Taiwan University, Prof. Jing-Tang Yang, from Department of Mechanical Engineering, National Taiwan University, and the Core Facility Center, Taipei Medical University for facility support.
Funding Information:
This work was supported by the Ministry of Science and Technology of Taiwan under grant numbers MOST 109-2636-E-038-003 , 108-2811-E-002-542 , and 108-2221-E-002-174-MY3 . We would also like to thank the NEMS Research Center, National Taiwan University, Prof. Jing-Tang Yang, from Department of Mechanical Engineering, National Taiwan University, and the Core Facility Center, Taipei Medical University for facility support.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - Microflow cytometry was previously pursued to achieve high-throughput cell analyses. It has advantages of parallelization and miniaturization capabilities that allow fewer detecting elements to simultaneously observe multiple channels. However, the major disadvantage is that it is difficult to precisely control parallel cell positions in each channel when cells pass through the detection area. In this study, we developed a multilayer microfluidic device, which has one sample flow inlet, two sheath flow inlets, and one flow outlet, which can achieve parallel sheath 3D flow focusing and fluorescence detection in 32 channels. The sample flow in each channel is focused and allows the cells or particles to pass through the detection zone one by one. Optimized flow rate ratios of the sample flow, lateral sheath flow, and circular sheath flow were found. The highest throughput of 106 cells/s was obtained using mammalian cells. The current cytometer, which incorporates a microoptic-integrated microfluidic device and newly developed optical system, was proven to have multicolor detection ability.
AB - Microflow cytometry was previously pursued to achieve high-throughput cell analyses. It has advantages of parallelization and miniaturization capabilities that allow fewer detecting elements to simultaneously observe multiple channels. However, the major disadvantage is that it is difficult to precisely control parallel cell positions in each channel when cells pass through the detection area. In this study, we developed a multilayer microfluidic device, which has one sample flow inlet, two sheath flow inlets, and one flow outlet, which can achieve parallel sheath 3D flow focusing and fluorescence detection in 32 channels. The sample flow in each channel is focused and allows the cells or particles to pass through the detection zone one by one. Optimized flow rate ratios of the sample flow, lateral sheath flow, and circular sheath flow were found. The highest throughput of 106 cells/s was obtained using mammalian cells. The current cytometer, which incorporates a microoptic-integrated microfluidic device and newly developed optical system, was proven to have multicolor detection ability.
KW - 3D microfluidics
KW - Flow cytometry
KW - High throughput
KW - Microoptics
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U2 - 10.1016/j.snb.2020.128255
DO - 10.1016/j.snb.2020.128255
M3 - Article
AN - SCOPUS:85085898068
SN - 0925-4005
VL - 320
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
M1 - 128255
ER -