Abstract
An HPLC assay is described for the determination of Z-butylidenephthalide (Z-Bdph) in plasma. Plasma samples were cleaned up by extraction with 2% chloroform in n-hexane. Z-Bdph was separated on a normal-phase silica column with a mobile phase of chloroform-n-hexane (1:1). The limit of quantitation with UV detection at 254 nm for Z-Bdph in plasma was 0.01 μg/ml. The recovery of Z-Bdph by organic solvent extraction of plasma was 99.5%. The intra-day and inter-day coefficients of variation and relative errors for Z-Bdph determination in plasma were both less than 10%. The present method was applied to pharmacokinetic studies of Z-Bdph in plasma after intravenous administration to rabbits.
Original language | English |
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Pages (from-to) | 379-382 |
Number of pages | 4 |
Journal | Journal of Chromatography B: Biomedical Applications |
Volume | 685 |
Issue number | 2 |
DOIs | |
Publication status | Published - Oct 25 1996 |
Keywords
- Z-Butylidenephthalide
ASJC Scopus subject areas
- General Chemistry