Determination of nimetazepam and 7-aminonimetazepam in human urine by using liquid chromatography-tandem mass spectrometry

Kuang Chuan Wang; Min Chi Cheng; Chin Lin Hsieh; Jung Fa Hsu; Jen Der Wu; Ching Kuo Lee

doi:10.1016/j.forsciint.2012.11.001

Determination of nimetazepam and 7-aminonimetazepam in human urine by using liquid chromatography-tandem mass spectrometry

Kuang Chuan Wang, Min Chi Cheng, Chin Lin Hsieh, Jung Fa Hsu, Jen Der Wu, Ching Kuo Lee

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

We report determination of metabolites of popular drugs of abuse, including nimetazepam and nitrazepam, in urine by using liquid chromatography/mass spectrometry. Nimetazepam and its metabolites, 7-aminonimetazepam and nitrazepam, were extracted by solid-phase extraction using a DAU cartridge. An ammonium acetate buffer solution (pH 4) and a Luna polar-RP column were selected as the mobile and stationary phase, respectively, for liquid chromatography. Mass spectrometry was used for analysis and was optimized for operation in the positive mode for all analytes. The urine specimens were screened for the presence of nimetazepam and its metabolites nitrazepam and 7-aminonimetazepam at a concentration of 0.1. ng/mL. Presence of 7-aminonimetazepam in the urine was an indicator of the subject being a probable abuser of nimetazepam.

Original language	English
Pages (from-to)	84-89
Number of pages	6
Journal	Forensic Science International
Volume	224
Issue number	1-3
DOIs	https://doi.org/10.1016/j.forsciint.2012.11.001
Publication status	Published - Jan 10 2013

Keywords

7-Aminonimetazepam
LC-MS
Nimetazepam
Nitrazepam

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1016/j.forsciint.2012.11.001

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title = "Determination of nimetazepam and 7-aminonimetazepam in human urine by using liquid chromatography-tandem mass spectrometry",

abstract = "We report determination of metabolites of popular drugs of abuse, including nimetazepam and nitrazepam, in urine by using liquid chromatography/mass spectrometry. Nimetazepam and its metabolites, 7-aminonimetazepam and nitrazepam, were extracted by solid-phase extraction using a DAU cartridge. An ammonium acetate buffer solution (pH 4) and a Luna polar-RP column were selected as the mobile and stationary phase, respectively, for liquid chromatography. Mass spectrometry was used for analysis and was optimized for operation in the positive mode for all analytes. The urine specimens were screened for the presence of nimetazepam and its metabolites nitrazepam and 7-aminonimetazepam at a concentration of 0.1. ng/mL. Presence of 7-aminonimetazepam in the urine was an indicator of the subject being a probable abuser of nimetazepam.",

keywords = "7-Aminonimetazepam, LC-MS, Nimetazepam, Nitrazepam",

author = "Wang, {Kuang Chuan} and Cheng, {Min Chi} and Hsieh, {Chin Lin} and Hsu, {Jung Fa} and Wu, {Jen Der} and Lee, {Ching Kuo}",

note = "Funding Information: This research was supported by the grant from the National Bureau of Controlled Drugs, DOH, ROC ( DOH97-NNB-1011 ). We are grateful to Ms. Shwu-Huey Wang for the NMR data acquisition in the Instrumentation Center of Taipei Medical University and the YungShin Group for supporting MS instrumentation. ",

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doi = "10.1016/j.forsciint.2012.11.001",

language = "English",

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pages = "84--89",

journal = "Forensic Science International",

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AU - Wang, Kuang Chuan

AU - Cheng, Min Chi

AU - Hsieh, Chin Lin

AU - Hsu, Jung Fa

AU - Wu, Jen Der

AU - Lee, Ching Kuo

N1 - Funding Information: This research was supported by the grant from the National Bureau of Controlled Drugs, DOH, ROC ( DOH97-NNB-1011 ). We are grateful to Ms. Shwu-Huey Wang for the NMR data acquisition in the Instrumentation Center of Taipei Medical University and the YungShin Group for supporting MS instrumentation.

PY - 2013/1/10

Y1 - 2013/1/10

N2 - We report determination of metabolites of popular drugs of abuse, including nimetazepam and nitrazepam, in urine by using liquid chromatography/mass spectrometry. Nimetazepam and its metabolites, 7-aminonimetazepam and nitrazepam, were extracted by solid-phase extraction using a DAU cartridge. An ammonium acetate buffer solution (pH 4) and a Luna polar-RP column were selected as the mobile and stationary phase, respectively, for liquid chromatography. Mass spectrometry was used for analysis and was optimized for operation in the positive mode for all analytes. The urine specimens were screened for the presence of nimetazepam and its metabolites nitrazepam and 7-aminonimetazepam at a concentration of 0.1. ng/mL. Presence of 7-aminonimetazepam in the urine was an indicator of the subject being a probable abuser of nimetazepam.

AB - We report determination of metabolites of popular drugs of abuse, including nimetazepam and nitrazepam, in urine by using liquid chromatography/mass spectrometry. Nimetazepam and its metabolites, 7-aminonimetazepam and nitrazepam, were extracted by solid-phase extraction using a DAU cartridge. An ammonium acetate buffer solution (pH 4) and a Luna polar-RP column were selected as the mobile and stationary phase, respectively, for liquid chromatography. Mass spectrometry was used for analysis and was optimized for operation in the positive mode for all analytes. The urine specimens were screened for the presence of nimetazepam and its metabolites nitrazepam and 7-aminonimetazepam at a concentration of 0.1. ng/mL. Presence of 7-aminonimetazepam in the urine was an indicator of the subject being a probable abuser of nimetazepam.

KW - 7-Aminonimetazepam

KW - LC-MS

KW - Nimetazepam

KW - Nitrazepam

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Determination of nimetazepam and 7-aminonimetazepam in human urine by using liquid chromatography-tandem mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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