TY - JOUR
T1 - Determination of ketamine and metabolites in urine by liquid chromatography-mass spectrometry
AU - Chen, Chung Yu
AU - Lee, Maw Rong
AU - Cheng, Fu Chou
AU - Wu, Gong Jhe
N1 - Funding Information:
Financial support from National Science Council of Taiwan under contract no. NSC 90-2113-M-005-029 and National Chung-Hsing University under contract no. TCVGH-NCHU91VH02 is acknowledged.
PY - 2007/5/15
Y1 - 2007/5/15
N2 - We have developed an analytical method by using liquid chromatography-mass spectrometry (LC-MS) to determine ketamine and its metabolites in urine. The ionization efficiency between two ionization modes (ESI and APCI) of LC-MS was compared to each other. An easy and simple sample preparation of urine samples was made by passing samples through a 0.22 μm PVDF syringe filter. The results indicated that the ionization efficiency of positive APCI mode is better than positive ESI mode for determination of trace ketamines. A wide linearity range of the research is from 5 to 250 ng mL-1 and the detection limits for ketamine, norketamine and dehydronorketamine were 0.95, 0.48 and 0.33 ng mL-1, respectively. The proposed method was tested by analyzing ketamine and metabolites in the urines of volunteers. The concentrations of ketamine, norketamine and dehydronorketamine are ranged of 5.4-131.0, 12.5-74.1 and 22.8-278.9 ng mL-1, respectively and the ketamines concentration profiles in human urine were also determined. The results demonstrate the suitability of liquid chromatography-mass spectrometry approach to analyze trace amount of ketamine and its metabolites in urine.
AB - We have developed an analytical method by using liquid chromatography-mass spectrometry (LC-MS) to determine ketamine and its metabolites in urine. The ionization efficiency between two ionization modes (ESI and APCI) of LC-MS was compared to each other. An easy and simple sample preparation of urine samples was made by passing samples through a 0.22 μm PVDF syringe filter. The results indicated that the ionization efficiency of positive APCI mode is better than positive ESI mode for determination of trace ketamines. A wide linearity range of the research is from 5 to 250 ng mL-1 and the detection limits for ketamine, norketamine and dehydronorketamine were 0.95, 0.48 and 0.33 ng mL-1, respectively. The proposed method was tested by analyzing ketamine and metabolites in the urines of volunteers. The concentrations of ketamine, norketamine and dehydronorketamine are ranged of 5.4-131.0, 12.5-74.1 and 22.8-278.9 ng mL-1, respectively and the ketamines concentration profiles in human urine were also determined. The results demonstrate the suitability of liquid chromatography-mass spectrometry approach to analyze trace amount of ketamine and its metabolites in urine.
KW - Atmospheric pressure chemical ionization
KW - Electrospray ionization
KW - Ketamine
KW - Liquid chromatography-mass spectrometry
KW - Metabolites
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U2 - 10.1016/j.talanta.2007.01.016
DO - 10.1016/j.talanta.2007.01.016
M3 - Article
C2 - 19071747
AN - SCOPUS:34247393171
SN - 0039-9140
VL - 72
SP - 1217
EP - 1222
JO - Talanta
JF - Talanta
IS - 3
ER -