Determination and pharmacokinetics of Di-(2-ethylhexyl) phthalate in rats by ultra performance liquid chromatography with tandem mass spectrometry

Wan Ling Chang-Liao; Mei Ling Hou; Li Wen Chang; Chia Jung Lee; Yin Meng Tsai; Lie Chwen Lin; Tung Hu Tsai

doi:10.3390/molecules180911452

Determination and pharmacokinetics of Di-(2-ethylhexyl) phthalate in rats by ultra performance liquid chromatography with tandem mass spectrometry

Wan Ling Chang-Liao, Mei Ling Hou, Li Wen Chang, Chia Jung Lee, Yin Meng Tsai, Lie Chwen Lin, Tung Hu Tsai

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Di-(2-ethylhexyl) phthalate (DEHP) is used to increase the flexibility of plastics for industrial products. However, the illegal use of the plasticizer DEHP in food and drinks has been reported in Taiwan in 2011. In order to assess the exact extent of the absorption of DEHP via the oral route, the aim of this study is to develop a reliable and validated ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method to evaluate the oral bioavailability of DEHP in rats. The optimal chromatographic separation of DEHP and butyl benzyl phthalate (BBP; used as internal standard) were achieved on a C₁₈ column. The mobile phase was consisted of 5 mM ammonium acetatemethanol (11:89, v/v) with a flow rate of 0.25 mL/min. The monitoring ion transitions were m/z 391.4 → 149.0 for DEHP and m/z 313.3 → 149.0 for BBP. The mean matrix effects of DEHP at low, medium and high concentrations were 94.5 ± 5.7% and 100.1 ± 2.3% in plasma and feces homogenate samples, respectively. In conclusion, the validated UPLCMS/ MS method is suitable for analyzing the rat plasma sample of DEHP and the oral bioavailability of DEHP was about 7% in rats.

Original language	English
Pages (from-to)	11452-11466
Number of pages	15
Journal	Molecules
Volume	18
Issue number	9
DOIs	https://doi.org/10.3390/molecules180911452
Publication status	Published - Sept 2013
Externally published	Yes

Keywords

Di-(2-ethylhexyl) phthalate (DEHP)
Fecal excretion
Pharmacokinetics
Phthalates
Plasticizer

ASJC Scopus subject areas

Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry

Access to Document

10.3390/molecules180911452

Cite this

@article{30c9d2e3c709448f99a777fa991066da,

title = "Determination and pharmacokinetics of Di-(2-ethylhexyl) phthalate in rats by ultra performance liquid chromatography with tandem mass spectrometry",

abstract = "Di-(2-ethylhexyl) phthalate (DEHP) is used to increase the flexibility of plastics for industrial products. However, the illegal use of the plasticizer DEHP in food and drinks has been reported in Taiwan in 2011. In order to assess the exact extent of the absorption of DEHP via the oral route, the aim of this study is to develop a reliable and validated ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method to evaluate the oral bioavailability of DEHP in rats. The optimal chromatographic separation of DEHP and butyl benzyl phthalate (BBP; used as internal standard) were achieved on a C18 column. The mobile phase was consisted of 5 mM ammonium acetatemethanol (11:89, v/v) with a flow rate of 0.25 mL/min. The monitoring ion transitions were m/z 391.4 → 149.0 for DEHP and m/z 313.3 → 149.0 for BBP. The mean matrix effects of DEHP at low, medium and high concentrations were 94.5 ± 5.7% and 100.1 ± 2.3% in plasma and feces homogenate samples, respectively. In conclusion, the validated UPLCMS/ MS method is suitable for analyzing the rat plasma sample of DEHP and the oral bioavailability of DEHP was about 7% in rats.",

keywords = "Di-(2-ethylhexyl) phthalate (DEHP), Fecal excretion, Pharmacokinetics, Phthalates, Plasticizer",

author = "Chang-Liao, {Wan Ling} and Hou, {Mei Ling} and Chang, {Li Wen} and Lee, {Chia Jung} and Tsai, {Yin Meng} and Lin, {Lie Chwen} and Tsai, {Tung Hu}",

year = "2013",

month = sep,

doi = "10.3390/molecules180911452",

language = "English",

volume = "18",

pages = "11452--11466",

journal = "Molecules",

issn = "1420-3049",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "9",

}

TY - JOUR

T1 - Determination and pharmacokinetics of Di-(2-ethylhexyl) phthalate in rats by ultra performance liquid chromatography with tandem mass spectrometry

AU - Chang-Liao, Wan Ling

AU - Hou, Mei Ling

AU - Chang, Li Wen

AU - Lee, Chia Jung

AU - Tsai, Yin Meng

AU - Lin, Lie Chwen

AU - Tsai, Tung Hu

PY - 2013/9

Y1 - 2013/9

N2 - Di-(2-ethylhexyl) phthalate (DEHP) is used to increase the flexibility of plastics for industrial products. However, the illegal use of the plasticizer DEHP in food and drinks has been reported in Taiwan in 2011. In order to assess the exact extent of the absorption of DEHP via the oral route, the aim of this study is to develop a reliable and validated ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method to evaluate the oral bioavailability of DEHP in rats. The optimal chromatographic separation of DEHP and butyl benzyl phthalate (BBP; used as internal standard) were achieved on a C18 column. The mobile phase was consisted of 5 mM ammonium acetatemethanol (11:89, v/v) with a flow rate of 0.25 mL/min. The monitoring ion transitions were m/z 391.4 → 149.0 for DEHP and m/z 313.3 → 149.0 for BBP. The mean matrix effects of DEHP at low, medium and high concentrations were 94.5 ± 5.7% and 100.1 ± 2.3% in plasma and feces homogenate samples, respectively. In conclusion, the validated UPLCMS/ MS method is suitable for analyzing the rat plasma sample of DEHP and the oral bioavailability of DEHP was about 7% in rats.

AB - Di-(2-ethylhexyl) phthalate (DEHP) is used to increase the flexibility of plastics for industrial products. However, the illegal use of the plasticizer DEHP in food and drinks has been reported in Taiwan in 2011. In order to assess the exact extent of the absorption of DEHP via the oral route, the aim of this study is to develop a reliable and validated ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method to evaluate the oral bioavailability of DEHP in rats. The optimal chromatographic separation of DEHP and butyl benzyl phthalate (BBP; used as internal standard) were achieved on a C18 column. The mobile phase was consisted of 5 mM ammonium acetatemethanol (11:89, v/v) with a flow rate of 0.25 mL/min. The monitoring ion transitions were m/z 391.4 → 149.0 for DEHP and m/z 313.3 → 149.0 for BBP. The mean matrix effects of DEHP at low, medium and high concentrations were 94.5 ± 5.7% and 100.1 ± 2.3% in plasma and feces homogenate samples, respectively. In conclusion, the validated UPLCMS/ MS method is suitable for analyzing the rat plasma sample of DEHP and the oral bioavailability of DEHP was about 7% in rats.

KW - Di-(2-ethylhexyl) phthalate (DEHP)

KW - Fecal excretion

KW - Pharmacokinetics

KW - Phthalates

KW - Plasticizer

UR - http://www.scopus.com/inward/record.url?scp=84885096113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885096113&partnerID=8YFLogxK

U2 - 10.3390/molecules180911452

DO - 10.3390/molecules180911452

M3 - Article

C2 - 24043141

AN - SCOPUS:84885096113

SN - 1420-3049

VL - 18

SP - 11452

EP - 11466

JO - Molecules

JF - Molecules

IS - 9

ER -

Determination and pharmacokinetics of Di-(2-ethylhexyl) phthalate in rats by ultra performance liquid chromatography with tandem mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this