Detection of SARS-associated coronavirus in throat wash and saliva in early diagnosis

Wei Kung Wang; Shey Ying Chen; I. Jung Liu; Yee Chun Chen; Hui Ling Chen; Chao Fu Yang; Pei Jer Chen; Shiou Hwei Yeh; Chuan Liang Kao; Li Min Huang; Po Ren Hsueh; Jann Tay Wang; Wang Hwei Sheng; Chi Tai Fang; Chien Ching Hung; Szu Min Hsieh; Chan Ping Su; Wen Chu Chiang; Jyh Yuan Yang; Jih Hui Lin; Szu Chia Hsieh; Hsien Ping Hu; Yu Ping Chiang; Jin Town Wang; Pan Chyr Yang; Shan Chwen Chang

doi:10.3201/eid1007.031113

Detection of SARS-associated coronavirus in throat wash and saliva in early diagnosis

Wei Kung Wang, Shey Ying Chen, I. Jung Liu, Yee Chun Chen, Hui Ling Chen, Chao Fu Yang, Pei Jer Chen, Shiou Hwei Yeh, Chuan Liang Kao, Li Min Huang, Po Ren Hsueh, Jann Tay Wang, Wang Hwei Sheng, Chi Tai Fang, Chien Ching Hung, Szu Min Hsieh, Chan Ping Su, Wen Chu Chiang, Jyh Yuan Yang, Jih Hui LinSzu Chia Hsieh, Hsien Ping Hu, Yu Ping Chiang, Jin Town Wang, Pan Chyr Yang, Shan Chwen Chang

Research output: Contribution to journal › Article › peer-review

185 Citations (Scopus)

Abstract

The severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is thought to be transmitted primarily through dispersal of droplets, but little is known about the load of SARS-CoV in oral droplets. We examined oral specimens, including throat wash and saliva, and found large amounts of SARS-CoV RNA in both throat wash (9.58 × 10² to 5.93 × 10⁶ copies/mL) and saliva (7.08 × 10³ to 6.38 × 10 ⁸ copies/mL) from all specimens of 17 consecutive probable SARS case-patients, supporting the possibility of transmission through oral droplets, Immunofluorescence study showed replication of SARS-CoV in the cells derived from throat wash, demonstrating the possibility of developing a convenient antigen detection assay. This finding, with the high detection rate a median of 4 days after disease onset and before the development of lung lesions in four patients, suggests that throat wash and saliva should be included in sample collection guidelines for SARS diagnosis.

Original language	English
Pages (from-to)	1213-1219
Number of pages	7
Journal	Emerging Infectious Diseases
Volume	10
Issue number	7
DOIs	https://doi.org/10.3201/eid1007.031113
Publication status	Published - Jan 1 2004
Externally published	Yes

ASJC Scopus subject areas

Epidemiology
Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3201/eid1007.031113

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Wang, W. K., Chen, S. Y., Liu, I. J., Chen, Y. C., Chen, H. L., Yang, C. F., Chen, P. J., Yeh, S. H., Kao, C. L., Huang, L. M., Hsueh, P. R., Wang, J. T., Sheng, W. H., Fang, C. T., Hung, C. C., Hsieh, S. M., Su, C. P., Chiang, W. C., Yang, J. Y., ... Chang, S. C. (2004). Detection of SARS-associated coronavirus in throat wash and saliva in early diagnosis. Emerging Infectious Diseases, 10(7), 1213-1219. https://doi.org/10.3201/eid1007.031113

Wang, WK, Chen, SY, Liu, IJ, Chen, YC, Chen, HL, Yang, CF, Chen, PJ, Yeh, SH, Kao, CL, Huang, LM, Hsueh, PR, Wang, JT, Sheng, WH, Fang, CT, Hung, CC, Hsieh, SM, Su, CP, Chiang, WC, Yang, JY, Lin, JH, Hsieh, SC, Hu, HP, Chiang, YP, Wang, JT, Yang, PC & Chang, SC 2004, 'Detection of SARS-associated coronavirus in throat wash and saliva in early diagnosis', Emerging Infectious Diseases, vol. 10, no. 7, pp. 1213-1219. https://doi.org/10.3201/eid1007.031113

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abstract = "The severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is thought to be transmitted primarily through dispersal of droplets, but little is known about the load of SARS-CoV in oral droplets. We examined oral specimens, including throat wash and saliva, and found large amounts of SARS-CoV RNA in both throat wash (9.58 × 102 to 5.93 × 106 copies/mL) and saliva (7.08 × 103 to 6.38 × 10 8 copies/mL) from all specimens of 17 consecutive probable SARS case-patients, supporting the possibility of transmission through oral droplets, Immunofluorescence study showed replication of SARS-CoV in the cells derived from throat wash, demonstrating the possibility of developing a convenient antigen detection assay. This finding, with the high detection rate a median of 4 days after disease onset and before the development of lung lesions in four patients, suggests that throat wash and saliva should be included in sample collection guidelines for SARS diagnosis.",

author = "Wang, {Wei Kung} and Chen, {Shey Ying} and Liu, {I. Jung} and Chen, {Yee Chun} and Chen, {Hui Ling} and Yang, {Chao Fu} and Chen, {Pei Jer} and Yeh, {Shiou Hwei} and Kao, {Chuan Liang} and Huang, {Li Min} and Hsueh, {Po Ren} and Wang, {Jann Tay} and Sheng, {Wang Hwei} and Fang, {Chi Tai} and Hung, {Chien Ching} and Hsieh, {Szu Min} and Su, {Chan Ping} and Chiang, {Wen Chu} and Yang, {Jyh Yuan} and Lin, {Jih Hui} and Hsieh, {Szu Chia} and Hu, {Hsien Ping} and Chiang, {Yu Ping} and Wang, {Jin Town} and Yang, {Pan Chyr} and Chang, {Shan Chwen}",

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AU - Chen, Shey Ying

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AU - Chen, Yee Chun

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AU - Yang, Chao Fu

AU - Chen, Pei Jer

AU - Yeh, Shiou Hwei

AU - Kao, Chuan Liang

AU - Huang, Li Min

AU - Hsueh, Po Ren

AU - Wang, Jann Tay

AU - Sheng, Wang Hwei

AU - Fang, Chi Tai

AU - Hung, Chien Ching

AU - Hsieh, Szu Min

AU - Su, Chan Ping

AU - Chiang, Wen Chu

AU - Yang, Jyh Yuan

AU - Lin, Jih Hui

AU - Hsieh, Szu Chia

AU - Hu, Hsien Ping

AU - Chiang, Yu Ping

AU - Wang, Jin Town

AU - Yang, Pan Chyr

AU - Chang, Shan Chwen

PY - 2004/1/1

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N2 - The severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is thought to be transmitted primarily through dispersal of droplets, but little is known about the load of SARS-CoV in oral droplets. We examined oral specimens, including throat wash and saliva, and found large amounts of SARS-CoV RNA in both throat wash (9.58 × 102 to 5.93 × 106 copies/mL) and saliva (7.08 × 103 to 6.38 × 10 8 copies/mL) from all specimens of 17 consecutive probable SARS case-patients, supporting the possibility of transmission through oral droplets, Immunofluorescence study showed replication of SARS-CoV in the cells derived from throat wash, demonstrating the possibility of developing a convenient antigen detection assay. This finding, with the high detection rate a median of 4 days after disease onset and before the development of lung lesions in four patients, suggests that throat wash and saliva should be included in sample collection guidelines for SARS diagnosis.

AB - The severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is thought to be transmitted primarily through dispersal of droplets, but little is known about the load of SARS-CoV in oral droplets. We examined oral specimens, including throat wash and saliva, and found large amounts of SARS-CoV RNA in both throat wash (9.58 × 102 to 5.93 × 106 copies/mL) and saliva (7.08 × 103 to 6.38 × 10 8 copies/mL) from all specimens of 17 consecutive probable SARS case-patients, supporting the possibility of transmission through oral droplets, Immunofluorescence study showed replication of SARS-CoV in the cells derived from throat wash, demonstrating the possibility of developing a convenient antigen detection assay. This finding, with the high detection rate a median of 4 days after disease onset and before the development of lung lesions in four patients, suggests that throat wash and saliva should be included in sample collection guidelines for SARS diagnosis.

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Detection of SARS-associated coronavirus in throat wash and saliva in early diagnosis

Abstract

ASJC Scopus subject areas

UN SDGs

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