Detection of K-ras point mutations in codons 12 and 13 in non-small cell lung cancers

Nan Yung Hsu; Chih Yi Chen; Chii Huei Wu; Tse Fia Liu; Po Cheung Kwam; Chung Ping Hsu; Jiun Yi Hsia; Wu Tsung Chang

Detection of K-ras point mutations in codons 12 and 13 in non-small cell lung cancers

Nan Yung Hsu, Chih Yi Chen, Chii Huei Wu, Tse Fia Liu, Po Cheung Kwam, Chung Ping Hsu, Jiun Yi Hsia, Wu Tsung Chang

Research output: Contribution to journal › Article › peer-review

Abstract

Point mutations of the K-ras gene have been reported in a wide variety of human tumors. By using polymerase chain reaction followed by direct DNA sequencing, we screened for point mutations at codons 12 and 13 of the K-ras gene in specimens obtained from fresh frozen tumors in 38 patients with non-small cell lung cancers. Point mutations were detected in two of 38 (5.3%) resected non-small cell lung cancers. Both of them were G to T transversions. One patient was found to have a K-ras codon 13 point mutation (GGC to TGC, gly to cys), while the other had a codon 12 point mutation (GGT to GTT, gly to val). Based on the limited numbers in this study, we found that the frequency of K-ras point mutations in codons 12 and 13 among Asian patients with lung adenocarcinomas was lower than that detected among Caucasian patients.

Original language	English
Pages (from-to)	741-745
Number of pages	5
Journal	Journal of the Formosan Medical Association
Volume	95
Issue number	10
Publication status	Published - 1996
Externally published	Yes

Keywords

K-ras gene
codon 12
codon 13
mutation
non-small cell lung cancer

ASJC Scopus subject areas

General Medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Detection of K-ras point mutations in codons 12 and 13 in non-small cell lung cancers",

abstract = "Point mutations of the K-ras gene have been reported in a wide variety of human tumors. By using polymerase chain reaction followed by direct DNA sequencing, we screened for point mutations at codons 12 and 13 of the K-ras gene in specimens obtained from fresh frozen tumors in 38 patients with non-small cell lung cancers. Point mutations were detected in two of 38 (5.3%) resected non-small cell lung cancers. Both of them were G to T transversions. One patient was found to have a K-ras codon 13 point mutation (GGC to TGC, gly to cys), while the other had a codon 12 point mutation (GGT to GTT, gly to val). Based on the limited numbers in this study, we found that the frequency of K-ras point mutations in codons 12 and 13 among Asian patients with lung adenocarcinomas was lower than that detected among Caucasian patients.",

keywords = "K-ras gene, codon 12, codon 13, mutation, non-small cell lung cancer",

author = "Hsu, {Nan Yung} and Chen, {Chih Yi} and Wu, {Chii Huei} and Liu, {Tse Fia} and Kwam, {Po Cheung} and Hsu, {Chung Ping} and Hsia, {Jiun Yi} and Chang, {Wu Tsung}",

year = "1996",

language = "English",

volume = "95",

pages = "741--745",

journal = "Journal of the Formosan Medical Association",

issn = "0929-6646",

publisher = "Elsevier Science Publishers B.V.",

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}

TY - JOUR

T1 - Detection of K-ras point mutations in codons 12 and 13 in non-small cell lung cancers

AU - Hsu, Nan Yung

AU - Chen, Chih Yi

AU - Wu, Chii Huei

AU - Liu, Tse Fia

AU - Kwam, Po Cheung

AU - Hsu, Chung Ping

AU - Hsia, Jiun Yi

AU - Chang, Wu Tsung

PY - 1996

Y1 - 1996

N2 - Point mutations of the K-ras gene have been reported in a wide variety of human tumors. By using polymerase chain reaction followed by direct DNA sequencing, we screened for point mutations at codons 12 and 13 of the K-ras gene in specimens obtained from fresh frozen tumors in 38 patients with non-small cell lung cancers. Point mutations were detected in two of 38 (5.3%) resected non-small cell lung cancers. Both of them were G to T transversions. One patient was found to have a K-ras codon 13 point mutation (GGC to TGC, gly to cys), while the other had a codon 12 point mutation (GGT to GTT, gly to val). Based on the limited numbers in this study, we found that the frequency of K-ras point mutations in codons 12 and 13 among Asian patients with lung adenocarcinomas was lower than that detected among Caucasian patients.

AB - Point mutations of the K-ras gene have been reported in a wide variety of human tumors. By using polymerase chain reaction followed by direct DNA sequencing, we screened for point mutations at codons 12 and 13 of the K-ras gene in specimens obtained from fresh frozen tumors in 38 patients with non-small cell lung cancers. Point mutations were detected in two of 38 (5.3%) resected non-small cell lung cancers. Both of them were G to T transversions. One patient was found to have a K-ras codon 13 point mutation (GGC to TGC, gly to cys), while the other had a codon 12 point mutation (GGT to GTT, gly to val). Based on the limited numbers in this study, we found that the frequency of K-ras point mutations in codons 12 and 13 among Asian patients with lung adenocarcinomas was lower than that detected among Caucasian patients.

KW - K-ras gene

KW - codon 12

KW - codon 13

KW - mutation

KW - non-small cell lung cancer

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M3 - Article

C2 - 8961670

AN - SCOPUS:0029852504

SN - 0929-6646

VL - 95

SP - 741

EP - 745

JO - Journal of the Formosan Medical Association

JF - Journal of the Formosan Medical Association

IS - 10

ER -

Detection of K-ras point mutations in codons 12 and 13 in non-small cell lung cancers

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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