Detection of Epstein-Barr virus infection and gene expression in human tumors by microarray analysis

Ching Li; Ruey Shyang Chen; Shang Kai Hung; Yuan Ti Lee; Ching Yu Yen; Yi Wen Lai; Ru Hsiu Teng; Jia Yan Huang; Yung Cheng Tang; Chao Ping Tung; Ta Tung Wei; Biehuoy Shieh; Shih Tung Liu

doi:10.1016/j.jviromet.2005.10.032

Detection of Epstein-Barr virus infection and gene expression in human tumors by microarray analysis

Ching Li, Ruey Shyang Chen, Shang Kai Hung, Yuan Ti Lee, Ching Yu Yen, Yi Wen Lai, Ru Hsiu Teng, Jia Yan Huang, Yung Cheng Tang, Chao Ping Tung, Ta Tung Wei, Biehuoy Shieh, Shih Tung Liu

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) genome-chips are employed to determine the EBV infection rate and to reveal the gene expression patterns of EBV in tumor biopsies. These chips are produced with 71 consecutive PCR-amplified EBV DNA fragments of 1-3 kbp covering the entire EBV genome. The specificity of the EBV-chips is determined by hybridizing the DNA on the chips with biotin-labeled cDNA probes reverse transcribed from the mRNA of P3HR1 cells, which were B-cell infected latently by EBV. Hybridization results revealed only the expression of EBNA1, EBNA2, EBER1 and EBER2 in these cells. On the other hand, EBV lytic genes are expressed after the cells are treated with 12-O-tetradecanoylphorbol-13- acetate and sodium butyrate to induce the EBV lytic cycle. Fourty-four tumor biopsies from different organs are assayed with these chips, which showed many defined and interesting EBV gene expression patterns. This study demonstrates that the EBV-chip is useful for screening infection with EBV in tumors, which may lead to insights into tumorigenesis associated with this virus.

Original language	English
Pages (from-to)	158-166
Number of pages	9
Journal	Journal of Virological Methods
Volume	133
Issue number	2
DOIs	https://doi.org/10.1016/j.jviromet.2005.10.032
Publication status	Published - May 2006
Externally published	Yes

Keywords

EBV-chip
Epstein-Barr virus
Microarray
Profiling gene expression pattern

ASJC Scopus subject areas

Virology

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10.1016/j.jviromet.2005.10.032

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@article{97e3c7615de7465c9c2e7eb76a327075,

title = "Detection of Epstein-Barr virus infection and gene expression in human tumors by microarray analysis",

abstract = "Epstein-Barr virus (EBV) genome-chips are employed to determine the EBV infection rate and to reveal the gene expression patterns of EBV in tumor biopsies. These chips are produced with 71 consecutive PCR-amplified EBV DNA fragments of 1-3 kbp covering the entire EBV genome. The specificity of the EBV-chips is determined by hybridizing the DNA on the chips with biotin-labeled cDNA probes reverse transcribed from the mRNA of P3HR1 cells, which were B-cell infected latently by EBV. Hybridization results revealed only the expression of EBNA1, EBNA2, EBER1 and EBER2 in these cells. On the other hand, EBV lytic genes are expressed after the cells are treated with 12-O-tetradecanoylphorbol-13- acetate and sodium butyrate to induce the EBV lytic cycle. Fourty-four tumor biopsies from different organs are assayed with these chips, which showed many defined and interesting EBV gene expression patterns. This study demonstrates that the EBV-chip is useful for screening infection with EBV in tumors, which may lead to insights into tumorigenesis associated with this virus.",

keywords = "EBV-chip, Epstein-Barr virus, Microarray, Profiling gene expression pattern",

author = "Ching Li and Chen, {Ruey Shyang} and Hung, {Shang Kai} and Lee, {Yuan Ti} and Yen, {Ching Yu} and Lai, {Yi Wen} and Teng, {Ru Hsiu} and Huang, {Jia Yan} and Tang, {Yung Cheng} and Tung, {Chao Ping} and Wei, {Ta Tung} and Biehuoy Shieh and Liu, {Shih Tung}",

note = "Funding Information: The authors wish to express our gratitude to Dr. Hong-Chen Chen at National Chung Hsing University and Dr. Tzong-Shin Tzai at National Cheng Kung University Medical College for providing tissue specimens. This research was support partly by funds from National Health Research Institutes of ROC (NHRI-EX91-8901SL), NSC94-3112-B-182-004 to S.T.L. and Chung Shan Medical University (CSMU 92-OM-B-018) to Y.T.L. and C.L. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2006",

month = may,

doi = "10.1016/j.jviromet.2005.10.032",

language = "English",

volume = "133",

pages = "158--166",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Detection of Epstein-Barr virus infection and gene expression in human tumors by microarray analysis

AU - Li, Ching

AU - Chen, Ruey Shyang

AU - Hung, Shang Kai

AU - Lee, Yuan Ti

AU - Yen, Ching Yu

AU - Lai, Yi Wen

AU - Teng, Ru Hsiu

AU - Huang, Jia Yan

AU - Tang, Yung Cheng

AU - Tung, Chao Ping

AU - Wei, Ta Tung

AU - Shieh, Biehuoy

AU - Liu, Shih Tung

N1 - Funding Information: The authors wish to express our gratitude to Dr. Hong-Chen Chen at National Chung Hsing University and Dr. Tzong-Shin Tzai at National Cheng Kung University Medical College for providing tissue specimens. This research was support partly by funds from National Health Research Institutes of ROC (NHRI-EX91-8901SL), NSC94-3112-B-182-004 to S.T.L. and Chung Shan Medical University (CSMU 92-OM-B-018) to Y.T.L. and C.L. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2006/5

Y1 - 2006/5

N2 - Epstein-Barr virus (EBV) genome-chips are employed to determine the EBV infection rate and to reveal the gene expression patterns of EBV in tumor biopsies. These chips are produced with 71 consecutive PCR-amplified EBV DNA fragments of 1-3 kbp covering the entire EBV genome. The specificity of the EBV-chips is determined by hybridizing the DNA on the chips with biotin-labeled cDNA probes reverse transcribed from the mRNA of P3HR1 cells, which were B-cell infected latently by EBV. Hybridization results revealed only the expression of EBNA1, EBNA2, EBER1 and EBER2 in these cells. On the other hand, EBV lytic genes are expressed after the cells are treated with 12-O-tetradecanoylphorbol-13- acetate and sodium butyrate to induce the EBV lytic cycle. Fourty-four tumor biopsies from different organs are assayed with these chips, which showed many defined and interesting EBV gene expression patterns. This study demonstrates that the EBV-chip is useful for screening infection with EBV in tumors, which may lead to insights into tumorigenesis associated with this virus.

AB - Epstein-Barr virus (EBV) genome-chips are employed to determine the EBV infection rate and to reveal the gene expression patterns of EBV in tumor biopsies. These chips are produced with 71 consecutive PCR-amplified EBV DNA fragments of 1-3 kbp covering the entire EBV genome. The specificity of the EBV-chips is determined by hybridizing the DNA on the chips with biotin-labeled cDNA probes reverse transcribed from the mRNA of P3HR1 cells, which were B-cell infected latently by EBV. Hybridization results revealed only the expression of EBNA1, EBNA2, EBER1 and EBER2 in these cells. On the other hand, EBV lytic genes are expressed after the cells are treated with 12-O-tetradecanoylphorbol-13- acetate and sodium butyrate to induce the EBV lytic cycle. Fourty-four tumor biopsies from different organs are assayed with these chips, which showed many defined and interesting EBV gene expression patterns. This study demonstrates that the EBV-chip is useful for screening infection with EBV in tumors, which may lead to insights into tumorigenesis associated with this virus.

KW - EBV-chip

KW - Epstein-Barr virus

KW - Microarray

KW - Profiling gene expression pattern

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AN - SCOPUS:33645299815

SN - 0166-0934

VL - 133

SP - 158

EP - 166

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 2

ER -

Detection of Epstein-Barr virus infection and gene expression in human tumors by microarray analysis

Abstract

Keywords

ASJC Scopus subject areas

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