Detection of cefotaxime-resistant CTX-M-3 in clinical isolates of Serratia marcescens

Mei Fen Tsou; Wen Liang Yu; Wan Hung Siu; Chizh Ju Chi; Chieh-Ju; Ching Chen Ssu; Lii Tzu Wu

doi:10.1007/BF02931608

Detection of cefotaxime-resistant CTX-M-3 in clinical isolates of Serratia marcescens

Mei Fen Tsou, Wen Liang Yu, Wan Hung Siu, Chizh Ju Chi, Chieh-Ju, Ching Chen Ssu, Lii Tzu Wu

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Strains of Serratia marcescens (isolated in a hospital during April and August 2000) resistant to ampicillin, chloramphenicol, trimethoprim- sulfamethoxazole, streptomycin, tetracycline, and gentamicin were characterized. Out of a total of 34 clinical isolates 6 (17.6 %) exhibited the extended spectrum β-lactamases (ESBL) resistance; they were also resistant to cefotaxime (minimum inhibitory concentration, MIC ≥ 128 μg/mL) but susceptible to imipenem (MIC ≤ 0.5 μg/mL). This multidrug resistance was shown to be transferred by a conjugative plasmid. Transconjugants revealed similar MIC profiles when compared to the parental strains. Isoelectric focusing revealed one major transferable β-lactamase (pI 8.4) which was further identified as CTX-M-3 by PCR and gene sequencing. The presence of strains with this type of ESBL showed the evolution of bla genes and their dissemination among at least three species of the family Enterobacteriaceae isolated within a single hospital. The predominance of CTX-M type enzymes found in this area of Taiwan appeared to be similar to that described in Poland.

Original language	English
Pages (from-to)	452-456
Number of pages	5
Journal	Folia Microbiologica
Volume	49
Issue number	4
DOIs	https://doi.org/10.1007/BF02931608
Publication status	Published - 2004

ASJC Scopus subject areas

Microbiology

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title = "Detection of cefotaxime-resistant CTX-M-3 in clinical isolates of Serratia marcescens",

abstract = "Strains of Serratia marcescens (isolated in a hospital during April and August 2000) resistant to ampicillin, chloramphenicol, trimethoprim- sulfamethoxazole, streptomycin, tetracycline, and gentamicin were characterized. Out of a total of 34 clinical isolates 6 (17.6 %) exhibited the extended spectrum β-lactamases (ESBL) resistance; they were also resistant to cefotaxime (minimum inhibitory concentration, MIC ≥ 128 μg/mL) but susceptible to imipenem (MIC ≤ 0.5 μg/mL). This multidrug resistance was shown to be transferred by a conjugative plasmid. Transconjugants revealed similar MIC profiles when compared to the parental strains. Isoelectric focusing revealed one major transferable β-lactamase (pI 8.4) which was further identified as CTX-M-3 by PCR and gene sequencing. The presence of strains with this type of ESBL showed the evolution of bla genes and their dissemination among at least three species of the family Enterobacteriaceae isolated within a single hospital. The predominance of CTX-M type enzymes found in this area of Taiwan appeared to be similar to that described in Poland.",

author = "Tsou, {Mei Fen} and Yu, {Wen Liang} and Siu, {Wan Hung} and Chi, {Chizh Ju} and Chieh-Ju and Ssu, {Ching Chen} and Wu, {Lii Tzu}",

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doi = "10.1007/BF02931608",

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T1 - Detection of cefotaxime-resistant CTX-M-3 in clinical isolates of Serratia marcescens

AU - Tsou, Mei Fen

AU - Yu, Wen Liang

AU - Siu, Wan Hung

AU - Chi, Chizh Ju

AU - Chieh-Ju,

AU - Ssu, Ching Chen

AU - Wu, Lii Tzu

PY - 2004

Y1 - 2004

N2 - Strains of Serratia marcescens (isolated in a hospital during April and August 2000) resistant to ampicillin, chloramphenicol, trimethoprim- sulfamethoxazole, streptomycin, tetracycline, and gentamicin were characterized. Out of a total of 34 clinical isolates 6 (17.6 %) exhibited the extended spectrum β-lactamases (ESBL) resistance; they were also resistant to cefotaxime (minimum inhibitory concentration, MIC ≥ 128 μg/mL) but susceptible to imipenem (MIC ≤ 0.5 μg/mL). This multidrug resistance was shown to be transferred by a conjugative plasmid. Transconjugants revealed similar MIC profiles when compared to the parental strains. Isoelectric focusing revealed one major transferable β-lactamase (pI 8.4) which was further identified as CTX-M-3 by PCR and gene sequencing. The presence of strains with this type of ESBL showed the evolution of bla genes and their dissemination among at least three species of the family Enterobacteriaceae isolated within a single hospital. The predominance of CTX-M type enzymes found in this area of Taiwan appeared to be similar to that described in Poland.

AB - Strains of Serratia marcescens (isolated in a hospital during April and August 2000) resistant to ampicillin, chloramphenicol, trimethoprim- sulfamethoxazole, streptomycin, tetracycline, and gentamicin were characterized. Out of a total of 34 clinical isolates 6 (17.6 %) exhibited the extended spectrum β-lactamases (ESBL) resistance; they were also resistant to cefotaxime (minimum inhibitory concentration, MIC ≥ 128 μg/mL) but susceptible to imipenem (MIC ≤ 0.5 μg/mL). This multidrug resistance was shown to be transferred by a conjugative plasmid. Transconjugants revealed similar MIC profiles when compared to the parental strains. Isoelectric focusing revealed one major transferable β-lactamase (pI 8.4) which was further identified as CTX-M-3 by PCR and gene sequencing. The presence of strains with this type of ESBL showed the evolution of bla genes and their dissemination among at least three species of the family Enterobacteriaceae isolated within a single hospital. The predominance of CTX-M type enzymes found in this area of Taiwan appeared to be similar to that described in Poland.

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Detection of cefotaxime-resistant CTX-M-3 in clinical isolates of Serratia marcescens

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