Detection of C-reactive protein in evanescent wave field usingmicroparticle-tracking velocimetry

Yu Jui Fan; Horn Jiunn Sheen; Yi Hsing Liu; Jing Fa Tsai; Tzu Heng Wu; Kuang Chong Wu; Shiming Lin

doi:10.1021/la102137j

Detection of C-reactive protein in evanescent wave field usingmicroparticle-tracking velocimetry

Yu Jui Fan, Horn Jiunn Sheen, Yi Hsing Liu, Jing Fa Tsai, Tzu Heng Wu, Kuang Chong Wu, Shiming Lin

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

A new technique is developed to measure the nanoparticles' Brownian motions by employing microparticle-tracking velocimetry (micro-PTV) in evanescent wave field, which can provide high signal-to-noise ratio images for analyzing nanoparticles' movements. This method enables real-time detection of C-reactive proteins (CRPs) during the rapid interaction between CRPs and anti-CRP-coated nanobeads as CRP concentrations are related to the nanobeads' Brownian velocity in the equilibrium state. The smallest observable nanobeads with 185 nm were utilized in this experiment to detect CRP concentrations as low as 0.1 μg/mL even in a high-viscosity solution. Further, the dissociation constant, KD, can be evaluated based on the experimental results.

Original language	English
Pages (from-to)	13751-13754
Number of pages	4
Journal	Langmuir
Volume	26
Issue number	17
DOIs	https://doi.org/10.1021/la102137j
Publication status	Published - Sept 7 2010
Externally published	Yes

ASJC Scopus subject areas

Electrochemistry
Condensed Matter Physics
Surfaces and Interfaces
General Materials Science
Spectroscopy

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10.1021/la102137j

Cite this

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title = "Detection of C-reactive protein in evanescent wave field usingmicroparticle-tracking velocimetry",

abstract = "A new technique is developed to measure the nanoparticles' Brownian motions by employing microparticle-tracking velocimetry (micro-PTV) in evanescent wave field, which can provide high signal-to-noise ratio images for analyzing nanoparticles' movements. This method enables real-time detection of C-reactive proteins (CRPs) during the rapid interaction between CRPs and anti-CRP-coated nanobeads as CRP concentrations are related to the nanobeads' Brownian velocity in the equilibrium state. The smallest observable nanobeads with 185 nm were utilized in this experiment to detect CRP concentrations as low as 0.1 μg/mL even in a high-viscosity solution. Further, the dissociation constant, KD, can be evaluated based on the experimental results.",

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T1 - Detection of C-reactive protein in evanescent wave field usingmicroparticle-tracking velocimetry

AU - Fan, Yu Jui

AU - Sheen, Horn Jiunn

AU - Liu, Yi Hsing

AU - Tsai, Jing Fa

AU - Wu, Tzu Heng

AU - Wu, Kuang Chong

AU - Lin, Shiming

PY - 2010/9/7

Y1 - 2010/9/7

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AB - A new technique is developed to measure the nanoparticles' Brownian motions by employing microparticle-tracking velocimetry (micro-PTV) in evanescent wave field, which can provide high signal-to-noise ratio images for analyzing nanoparticles' movements. This method enables real-time detection of C-reactive proteins (CRPs) during the rapid interaction between CRPs and anti-CRP-coated nanobeads as CRP concentrations are related to the nanobeads' Brownian velocity in the equilibrium state. The smallest observable nanobeads with 185 nm were utilized in this experiment to detect CRP concentrations as low as 0.1 μg/mL even in a high-viscosity solution. Further, the dissociation constant, KD, can be evaluated based on the experimental results.

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Detection of C-reactive protein in evanescent wave field usingmicroparticle-tracking velocimetry

Abstract

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