TY - JOUR
T1 - Detection and phenotyping of circulating tumor cells in high-risk localized prostate cancer
AU - Pal, Sumanta K.
AU - He, Miaoling
AU - Wilson, Timothy
AU - Liu, Xueli
AU - Zhang, Keqiang
AU - Carmichael, Courtney
AU - Torres, Alejandra
AU - Hernandez, Sonya
AU - Lau, Clayton
AU - Agarwal, Neeraj
AU - Kawachi, Mark
AU - Yen, Yun
AU - Jones, Jeremy O.
N1 - Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Background In this study, we aimed to determine the feasibility of identifying CTCs in patients with HRLPC, using a modified isolation procedure using the CellSearch (Veridex) platform, and to assess the expression of stem cell and epithelial-mesenchymal transition (EMT) markers on the CTCs. Patients and Methods Thirty-five patients with HRLPC who had chosen prostatectomy for definitive management were prospectively identified. After obtaining consent, four 30-mL blood draws were performed, 2 before surgery and 2 after surgery. The CTC-containing fraction was Ficoll-purified and transferred to a CellSave (Veridex) tube containing dilution buffer before standard enumeration using the CellSearch system. Loss of E-cadherin expression, a marker of EMT, and CD133, a putative prostate cancer stem cell marker, were characterized using the open channel of the CellSearch platform. CTC fragments were also enumerated. Results Using the modified methodology, CTCs were detectable in 49% of patients before surgery. Although no correlation between CTC count and biochemical recurrence (BR) was observed, the percentages of CD133 and E-cadherin-positive CTC fragments were associated with BR at 1 year. Conclusion Our results suggest that further research into the development of CTCs as prognostic biomarkers in HRLPC is warranted.
AB - Background In this study, we aimed to determine the feasibility of identifying CTCs in patients with HRLPC, using a modified isolation procedure using the CellSearch (Veridex) platform, and to assess the expression of stem cell and epithelial-mesenchymal transition (EMT) markers on the CTCs. Patients and Methods Thirty-five patients with HRLPC who had chosen prostatectomy for definitive management were prospectively identified. After obtaining consent, four 30-mL blood draws were performed, 2 before surgery and 2 after surgery. The CTC-containing fraction was Ficoll-purified and transferred to a CellSave (Veridex) tube containing dilution buffer before standard enumeration using the CellSearch system. Loss of E-cadherin expression, a marker of EMT, and CD133, a putative prostate cancer stem cell marker, were characterized using the open channel of the CellSearch platform. CTC fragments were also enumerated. Results Using the modified methodology, CTCs were detectable in 49% of patients before surgery. Although no correlation between CTC count and biochemical recurrence (BR) was observed, the percentages of CD133 and E-cadherin-positive CTC fragments were associated with BR at 1 year. Conclusion Our results suggest that further research into the development of CTCs as prognostic biomarkers in HRLPC is warranted.
KW - CD133
KW - CellSearch
KW - EMT
KW - Fragment
KW - Stem cell
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UR - http://www.scopus.com/inward/citedby.url?scp=84924551706&partnerID=8YFLogxK
U2 - 10.1016/j.clgc.2014.08.014
DO - 10.1016/j.clgc.2014.08.014
M3 - Article
C2 - 25450039
AN - SCOPUS:84924551706
SN - 1558-7673
VL - 13
SP - 130
EP - 136
JO - Clinical Genitourinary Cancer
JF - Clinical Genitourinary Cancer
IS - 2
ER -