TY - JOUR
T1 - Design of Fluorescence-Enhanced Silver Nanoisland Chips for High-Throughput and Rapid Arsenite Assay
AU - Yen, Hung-Chi
AU - Kuo, Tsung-Rong
AU - Huang, Min-Hui
AU - Huang, Hao-Kang
AU - Chen, Chia-Chun
N1 - Funding Information:
This work was supported by the program of Ministry of Science and Technology (MOST 106-2113-M-003-014-MY3). We would like to acknowledge Dr. Chi-Ming Lee for his excellent technical support at the TMU Core Facility Center.
Publisher Copyright:
© 2020 American Chemical Society.
PY - 2020/8/11
Y1 - 2020/8/11
N2 - High-throughput and rapid arsenite (As(III)) monitoring is an urgent task to deal with the critical threat from As(III) contamination in the environment. In this study, an effective, portable, and sensitive As(III) assay was developed using the plasmonic silver (pAg) chips for As(III) detection. The pAg chips were fabricated by a simple seed-mediated method to grow the silver nanoisland films (Ag-NIFs) with the compact nanoislands and adjustable interisland gaps on the large-sized substrates. With appropriate surface functionalization and optimal chip manufacturing, Cy7.5 fluorescence dye can be immobilized on the surface of Ag-NIFs in the presence of As(III) to output the enhanced fluorescence signals up to 10-fold and improve the detection limit of As(III) less than 10 ppb. According to our results, the high-throughput detection measurements and wide dynamic range over 4 orders of magnitude implied the broad prospects of pAg chips in fluorescence-enhanced assays. The proposed As(III) assay has shown great opportunities for the practical application of ultratrace As(III) monitoring.
AB - High-throughput and rapid arsenite (As(III)) monitoring is an urgent task to deal with the critical threat from As(III) contamination in the environment. In this study, an effective, portable, and sensitive As(III) assay was developed using the plasmonic silver (pAg) chips for As(III) detection. The pAg chips were fabricated by a simple seed-mediated method to grow the silver nanoisland films (Ag-NIFs) with the compact nanoislands and adjustable interisland gaps on the large-sized substrates. With appropriate surface functionalization and optimal chip manufacturing, Cy7.5 fluorescence dye can be immobilized on the surface of Ag-NIFs in the presence of As(III) to output the enhanced fluorescence signals up to 10-fold and improve the detection limit of As(III) less than 10 ppb. According to our results, the high-throughput detection measurements and wide dynamic range over 4 orders of magnitude implied the broad prospects of pAg chips in fluorescence-enhanced assays. The proposed As(III) assay has shown great opportunities for the practical application of ultratrace As(III) monitoring.
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U2 - 10.1021/acsomega.0c02533
DO - 10.1021/acsomega.0c02533
M3 - Article
C2 - 32803072
SN - 2470-1343
VL - 5
SP - 19771
EP - 19777
JO - ACS Omega
JF - ACS Omega
IS - 31
ER -