Abstract
Background It has been found that T H1-related cytokines can decrease the accumulation of eosinophils in lung tissue and relieve airway constriction. Objective Dendritic cells (DCs) have been found to prime naive T-helper cells efficiently. In this study, DCs infected with T H1 cytokine-expressing adenovirus can be used to induce antigen-specific T H1 cells for treatment in an animal model of asthma. Methods Cytokine gene-modulated DCs pulsed with ovalbumin antigen (OVA) were injected intravenously into naive mice 1 week before sensitization with OVA antigen. The mice were then monitored for OVA-specific IgE, airway inflammatory cell infiltration, and airway hyperresponsiveness in the study. Results Significant levels of IL-12 or IL-18 were expressed by Ad-IL-12 or Ad-IL-18 infected, bone marrow-derived DCs. Ad-IL-12 and Ad-IL-18 co-infected DCs effectively, decreasing sera anti-OVA IgE antibody levels, lung eosinophilia, and airway hyperresponsiveness. Conclusion We concluded that DCs modulated by T H1-prone cytokine-expressing adenoviruses can alleviate T H2-type airway inflammation in a murine model and can provide possible therapeutic application for DCs in asthma.
Original language | English |
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Pages (from-to) | 88-96 |
Number of pages | 9 |
Journal | Journal of Allergy and Clinical Immunology |
Volume | 114 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jul 2004 |
Keywords
- AHR
- Airway hyperresponsiveness
- Asthma animal model
- BAL
- Bronchoalveolar lavage
- DCs
- Dendritic cells
- GFP
- Green fluorescence protein
- MOI
- Multiplicity of infection
- OVA
- Ovalbumin
- cytokine expressing adenovirus
- dendritic cell
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology