Defective innate immune responses to respiratory syncytial virus infection in ovalbumin-sensitized mice

Shen Hao Lai, Sui Ling Liao, Kin Sun Wong, Tzou Yien Lin

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Background/Purpose Respiratory viral infections have frequently been reported to closely correlate with asthma exacerbations. Distinctive expression of cytokine/chemokine and anomalous responses of innate immunity induced by respiratory viral infections were suggested to play a key role. This study further evaluates the effects of airway sensitization on innate immunity in response to different viruses. Methods Murine sensitization was established using an ovalbumin (OVA) sensitization model. Mice were subsequently infected with either respiratory syncytial virus (RSV) or human metapneumovirus (hMPV). Type I interferon (IFN), cytokines, and chemokines were measured in bronchoalveolar lavage (BAL) fluid. Pulmonary tissue samples were collected for the analysis of viral titers and type I IFN signal transcriptors. Results Distinct expressions of cytokine/chemokine responses after viral infection were also found in mice with OVA sensitization. A significant increase of virus replication was found in lungs of RSV-infected sensitized mice. The increment of RSV titer was associated with the decreased levels of type I IFN. Although Toll-like receptor 3 (TLR3) expression was significantly increased in the lungs, the key signal transcriptor, IFN regulatory factor 3, was significantly suppressed in the RSV-infected sensitized mice. Conclusion A defective antiviral innate response was observed in the murine respiratory allergy model. Suppressed expression of IFN signal transcriptor contributes to decreased production of type I IFN. The defective innate immune response might result in acute viral exacerbations of allergic airway diseases.

Original languageEnglish
Pages (from-to)17-25
Number of pages9
JournalJournal of Microbiology, Immunology and Infection
Issue number1
Publication statusPublished - Feb 1 2017
Externally publishedYes


  • asthma
  • human metapneumovirus
  • innate immunity
  • mice
  • respiratory syncytial virus

ASJC Scopus subject areas

  • Immunology and Allergy
  • General Immunology and Microbiology
  • Microbiology (medical)
  • Infectious Diseases


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