Decreased inflammatory response in Toll-like receptor 2 knockout mice is associated with exacerbated Pneumocystis pneumonia

Shao Hung Wang; Chen Zhang; Mark E. Lasbury; Chung Ping Liao; Pamela J. Durant; Dennis Tschang; Chao Hung Lee

doi:10.1016/j.micinf.2007.12.014

Decreased inflammatory response in Toll-like receptor 2 knockout mice is associated with exacerbated Pneumocystis pneumonia

Shao Hung Wang, Chen Zhang, Mark E. Lasbury, Chung Ping Liao, Pamela J. Durant, Dennis Tschang, Chao Hung Lee

Graduate Institute of Medical Sciences

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Pneumocystis pneumonia (PcP) is marked by substantial inflammatory damage to the lung. We have found that Toll-like receptor 2 (TLR2) mediates macrophage inflammatory responses to Pneumocystis and hypothesized that TLR2 deficiency would lead to less severe inflammation and milder lung injury during PcP. Histopathology examination showed that TLR2-/- mice with PcP indeed exhibited milder pulmonary inflammation. TLR2-/- mouse lungs contained less TNF-α and displayed lower levels of NF-κB activation during PcP. However, TLR2-/- mice with PcP displayed increased severity in symptoms and organism burden. The increased organism burden is likely due to defects in protective mechanisms in TLR2-/- mice. mRNA levels of the inducible nitric oxide synthase and NADPH oxidase p47phox, as well as nitric oxide levels in the lungs, were decreased in TLR2-/- PcP mice. Taken together, this study shows that TLR2-mediated inflammatory responses contribute to a certain degree to the clearance of Pneumocystis organism in mice.

Original language	English
Pages (from-to)	334-341
Number of pages	8
Journal	Microbes and Infection
Volume	10
Issue number	4
DOIs	https://doi.org/10.1016/j.micinf.2007.12.014
Publication status	Published - Apr 2008

Keywords

Inflammation
Pneumocystis
Toll-like receptor 2

ASJC Scopus subject areas

Microbiology
Immunology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.micinf.2007.12.014

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title = "Decreased inflammatory response in Toll-like receptor 2 knockout mice is associated with exacerbated Pneumocystis pneumonia",

abstract = "Pneumocystis pneumonia (PcP) is marked by substantial inflammatory damage to the lung. We have found that Toll-like receptor 2 (TLR2) mediates macrophage inflammatory responses to Pneumocystis and hypothesized that TLR2 deficiency would lead to less severe inflammation and milder lung injury during PcP. Histopathology examination showed that TLR2-/- mice with PcP indeed exhibited milder pulmonary inflammation. TLR2-/- mouse lungs contained less TNF-α and displayed lower levels of NF-κB activation during PcP. However, TLR2-/- mice with PcP displayed increased severity in symptoms and organism burden. The increased organism burden is likely due to defects in protective mechanisms in TLR2-/- mice. mRNA levels of the inducible nitric oxide synthase and NADPH oxidase p47phox, as well as nitric oxide levels in the lungs, were decreased in TLR2-/- PcP mice. Taken together, this study shows that TLR2-mediated inflammatory responses contribute to a certain degree to the clearance of Pneumocystis organism in mice.",

keywords = "Inflammation, Pneumocystis, Toll-like receptor 2",

author = "Wang, {Shao Hung} and Chen Zhang and Lasbury, {Mark E.} and Liao, {Chung Ping} and Durant, {Pamela J.} and Dennis Tschang and Lee, {Chao Hung}",

note = "Funding Information: The study was supported by grants from the National Institutes of Health (RO1 HL65170 and RO1 AI062259).",

year = "2008",

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doi = "10.1016/j.micinf.2007.12.014",

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AU - Wang, Shao Hung

AU - Zhang, Chen

AU - Lasbury, Mark E.

AU - Liao, Chung Ping

AU - Durant, Pamela J.

AU - Tschang, Dennis

AU - Lee, Chao Hung

N1 - Funding Information: The study was supported by grants from the National Institutes of Health (RO1 HL65170 and RO1 AI062259).

PY - 2008/4

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N2 - Pneumocystis pneumonia (PcP) is marked by substantial inflammatory damage to the lung. We have found that Toll-like receptor 2 (TLR2) mediates macrophage inflammatory responses to Pneumocystis and hypothesized that TLR2 deficiency would lead to less severe inflammation and milder lung injury during PcP. Histopathology examination showed that TLR2-/- mice with PcP indeed exhibited milder pulmonary inflammation. TLR2-/- mouse lungs contained less TNF-α and displayed lower levels of NF-κB activation during PcP. However, TLR2-/- mice with PcP displayed increased severity in symptoms and organism burden. The increased organism burden is likely due to defects in protective mechanisms in TLR2-/- mice. mRNA levels of the inducible nitric oxide synthase and NADPH oxidase p47phox, as well as nitric oxide levels in the lungs, were decreased in TLR2-/- PcP mice. Taken together, this study shows that TLR2-mediated inflammatory responses contribute to a certain degree to the clearance of Pneumocystis organism in mice.

AB - Pneumocystis pneumonia (PcP) is marked by substantial inflammatory damage to the lung. We have found that Toll-like receptor 2 (TLR2) mediates macrophage inflammatory responses to Pneumocystis and hypothesized that TLR2 deficiency would lead to less severe inflammation and milder lung injury during PcP. Histopathology examination showed that TLR2-/- mice with PcP indeed exhibited milder pulmonary inflammation. TLR2-/- mouse lungs contained less TNF-α and displayed lower levels of NF-κB activation during PcP. However, TLR2-/- mice with PcP displayed increased severity in symptoms and organism burden. The increased organism burden is likely due to defects in protective mechanisms in TLR2-/- mice. mRNA levels of the inducible nitric oxide synthase and NADPH oxidase p47phox, as well as nitric oxide levels in the lungs, were decreased in TLR2-/- PcP mice. Taken together, this study shows that TLR2-mediated inflammatory responses contribute to a certain degree to the clearance of Pneumocystis organism in mice.

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KW - Pneumocystis

KW - Toll-like receptor 2

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Decreased inflammatory response in Toll-like receptor 2 knockout mice is associated with exacerbated Pneumocystis pneumonia

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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