DC-SIGN mediates avian H5N1 influenza virus infection in cis and in trans

Sheng Fan Wang; Jason C. Huang; Yuan Ming Lee; Shih Jen Liu; Yu Jiun Chan; Yat Pang Chau; Pele Chong; Yi Ming Arthur Chen

doi:10.1016/j.bbrc.2008.06.078

DC-SIGN mediates avian H5N1 influenza virus infection in cis and in trans

Sheng Fan Wang, Jason C. Huang, Yuan Ming Lee, Shih Jen Liu, Yu Jiun Chan, Yat Pang Chau, Pele Chong, Yi Ming Arthur Chen

Research output: Contribution to journal › Article › peer-review

72 Citations (Scopus)

Abstract

DC-SIGN, a C-type lectin receptor expressed in dendritic cells (DCs), has been identified as a receptor for human immunodeficiency virus type 1, hepatitis C virus, Ebola virus, cytomegalovirus, dengue virus, and the SARS coronavirus. We used H5N1 pseudotyped and reverse-genetics (RG) virus particles to study their ability to bind with DC-SIGN. Electronic microscopy and functional assay results indicate that pseudotyped viruses containing both HA and NA proteins express hemagglutination and are capable of infecting cells expressing α-2,3-linked sialic acid receptors. Results from a capture assay show that DC-SIGN-expressing cells (including B-THP-1/DC-SIGN and T-THP-1/DC-SIGN) and peripheral blood dendritic cells are capable of transferring H5N1 pseudotyped and RG virus particles to target cells; this action can be blocked by anti-DC-SIGN monoclonal antibodies. In summary, (a) DC-SIGN acts as a capture or attachment molecule for avian H5N1 virus, and (b) DC-SIGN mediates infections in cis and in trans.

Original language	English
Pages (from-to)	561-566
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	373
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2008.06.078
Publication status	Published - Sept 5 2008
Externally published	Yes

Keywords

DC-SIGN
Dendritic cells
H5N1
Pseudotyped virus

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2008.06.078

Cite this

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title = "DC-SIGN mediates avian H5N1 influenza virus infection in cis and in trans",

abstract = "DC-SIGN, a C-type lectin receptor expressed in dendritic cells (DCs), has been identified as a receptor for human immunodeficiency virus type 1, hepatitis C virus, Ebola virus, cytomegalovirus, dengue virus, and the SARS coronavirus. We used H5N1 pseudotyped and reverse-genetics (RG) virus particles to study their ability to bind with DC-SIGN. Electronic microscopy and functional assay results indicate that pseudotyped viruses containing both HA and NA proteins express hemagglutination and are capable of infecting cells expressing α-2,3-linked sialic acid receptors. Results from a capture assay show that DC-SIGN-expressing cells (including B-THP-1/DC-SIGN and T-THP-1/DC-SIGN) and peripheral blood dendritic cells are capable of transferring H5N1 pseudotyped and RG virus particles to target cells; this action can be blocked by anti-DC-SIGN monoclonal antibodies. In summary, (a) DC-SIGN acts as a capture or attachment molecule for avian H5N1 virus, and (b) DC-SIGN mediates infections in cis and in trans.",

keywords = "DC-SIGN, Dendritic cells, H5N1, Pseudotyped virus",

author = "Wang, {Sheng Fan} and Huang, {Jason C.} and Lee, {Yuan Ming} and Liu, {Shih Jen} and Chan, {Yu Jiun} and Chau, {Yat Pang} and Pele Chong and Chen, {Yi Ming Arthur}",

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TY - JOUR

T1 - DC-SIGN mediates avian H5N1 influenza virus infection in cis and in trans

AU - Wang, Sheng Fan

AU - Huang, Jason C.

AU - Lee, Yuan Ming

AU - Liu, Shih Jen

AU - Chan, Yu Jiun

AU - Chau, Yat Pang

AU - Chong, Pele

AU - Chen, Yi Ming Arthur

PY - 2008/9/5

Y1 - 2008/9/5

N2 - DC-SIGN, a C-type lectin receptor expressed in dendritic cells (DCs), has been identified as a receptor for human immunodeficiency virus type 1, hepatitis C virus, Ebola virus, cytomegalovirus, dengue virus, and the SARS coronavirus. We used H5N1 pseudotyped and reverse-genetics (RG) virus particles to study their ability to bind with DC-SIGN. Electronic microscopy and functional assay results indicate that pseudotyped viruses containing both HA and NA proteins express hemagglutination and are capable of infecting cells expressing α-2,3-linked sialic acid receptors. Results from a capture assay show that DC-SIGN-expressing cells (including B-THP-1/DC-SIGN and T-THP-1/DC-SIGN) and peripheral blood dendritic cells are capable of transferring H5N1 pseudotyped and RG virus particles to target cells; this action can be blocked by anti-DC-SIGN monoclonal antibodies. In summary, (a) DC-SIGN acts as a capture or attachment molecule for avian H5N1 virus, and (b) DC-SIGN mediates infections in cis and in trans.

AB - DC-SIGN, a C-type lectin receptor expressed in dendritic cells (DCs), has been identified as a receptor for human immunodeficiency virus type 1, hepatitis C virus, Ebola virus, cytomegalovirus, dengue virus, and the SARS coronavirus. We used H5N1 pseudotyped and reverse-genetics (RG) virus particles to study their ability to bind with DC-SIGN. Electronic microscopy and functional assay results indicate that pseudotyped viruses containing both HA and NA proteins express hemagglutination and are capable of infecting cells expressing α-2,3-linked sialic acid receptors. Results from a capture assay show that DC-SIGN-expressing cells (including B-THP-1/DC-SIGN and T-THP-1/DC-SIGN) and peripheral blood dendritic cells are capable of transferring H5N1 pseudotyped and RG virus particles to target cells; this action can be blocked by anti-DC-SIGN monoclonal antibodies. In summary, (a) DC-SIGN acts as a capture or attachment molecule for avian H5N1 virus, and (b) DC-SIGN mediates infections in cis and in trans.

KW - DC-SIGN

KW - Dendritic cells

KW - H5N1

KW - Pseudotyped virus

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DC-SIGN mediates avian H5N1 influenza virus infection in cis and in trans

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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