TY - JOUR
T1 - Daphnetin
T2 - A novel blue-green photonic switch for disodium phosphates that allows monitoring of polymerase chain reactions
AU - Pandith, Anup
AU - Koo, Jachoon
AU - Seo, Young Jun
N1 - Funding Information:
This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) ( 2017R1A2B4002398 ), funded by the Republic of Korea and Research Base Construction Fund Support Program, funded by Chonbuk National University in 2017.
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/11/5
Y1 - 2018/11/5
N2 - This paper describes the very simple and robust ratiometric photonic switching properties of daphnetin (DP) toward HPO4 2− ions selectively in complex biological fluids, without any interference from other relevant anions under physiological conditions. The sensing ability of DP toward HPO4 2− ions was first demonstrated using UV–Vis and fluorescence spectroscopy, dynamic light scattering (DLS), and one- and two-dimensional NMR spectroscopy. DP can detect HPO4 2− ions at concentrations up to the sub-micromolar/nanomolar level very effectively, with a ratiometric response resulting from intramolecular charge transfer aided by aggregated-induced emission. The interactions between DP and HPO4 2− ions resulted in new bands appearing in the UV–Vis (at 385 nm) and emission (at 535 nm) spectra. The noncovalently held HPO4 2− ions induced pronounced specific aggregation of DP molecules, resulting in the new excimer band at 535 nm while retaining the monomer band centered at 445 nm. In contrast, reciprocal absorptivity changes were observed at 320 and 385 nm, with exponential decrements and increments, respectively. This probe could effectively monitor the consumption of dNTPs during various cycles of the polymerase chain reaction performed with relatively short oligonucleotides as well as genomic DNA from Agrobacterium tumefaciens (AcH5α strain).
AB - This paper describes the very simple and robust ratiometric photonic switching properties of daphnetin (DP) toward HPO4 2− ions selectively in complex biological fluids, without any interference from other relevant anions under physiological conditions. The sensing ability of DP toward HPO4 2− ions was first demonstrated using UV–Vis and fluorescence spectroscopy, dynamic light scattering (DLS), and one- and two-dimensional NMR spectroscopy. DP can detect HPO4 2− ions at concentrations up to the sub-micromolar/nanomolar level very effectively, with a ratiometric response resulting from intramolecular charge transfer aided by aggregated-induced emission. The interactions between DP and HPO4 2− ions resulted in new bands appearing in the UV–Vis (at 385 nm) and emission (at 535 nm) spectra. The noncovalently held HPO4 2− ions induced pronounced specific aggregation of DP molecules, resulting in the new excimer band at 535 nm while retaining the monomer band centered at 445 nm. In contrast, reciprocal absorptivity changes were observed at 320 and 385 nm, with exponential decrements and increments, respectively. This probe could effectively monitor the consumption of dNTPs during various cycles of the polymerase chain reaction performed with relatively short oligonucleotides as well as genomic DNA from Agrobacterium tumefaciens (AcH5α strain).
KW - Aggregation-induced emission
KW - Daphnetin
KW - Excimer
KW - Intramolecular charge transfer
KW - Polymerase chain reaction
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U2 - 10.1016/j.saa.2018.06.085
DO - 10.1016/j.saa.2018.06.085
M3 - Article
C2 - 29980064
AN - SCOPUS:85049340001
SN - 1386-1425
VL - 204
SP - 620
EP - 628
JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
ER -