TY - JOUR
T1 - Daily coordination of orexinergic gating in the rat superior colliculus—Implications for intrinsic clock activities in the visual system
AU - Chrobok, Lukasz
AU - Jeczmien-Lazur, Jagoda Stanislawa
AU - Bubka, Monika
AU - Pradel, Kamil
AU - Klekocinska, Aleksandra
AU - Klich, Jasmin Daniela
AU - Ridla Rahim, Amalia
AU - Myung, Jihwan
AU - Kepczynski, Mariusz
AU - Lewandowski, Marian Henryk
N1 - Funding Information:
This work was financially supported by a project “Sonatina 2” 2018/28/C/NZ4/00099 given to LC and “Preludium 14” 2017/27/N/NZ4/00785 to KP from the Polish National Science Centre. KP was additionally supported by “Etiuda 8” doctoral scholarship 2020/36/T/NZ4/00341. The research was carried out using equipment purchased through financial support from the European Regional Development Fund in the framework of the Polish Innovation Economy Operational Program (contract No. POIG.02.01.00‐12‐023/08). JM was supported by the Taiwan Ministry of Science and Technology (109‐2320‐B‐038‐020, 109‐2314‐B‐038‐071, 109‐2314‐B‐038‐106‐MY3, 108‐2321‐B‐006‐023‐MY2, 107‐2410‐H‐038‐004‐MY2), the Higher Education Sprout Project by the Taiwan Ministry of Education (DP2‐109‐21121‐01‐N‐01, DP2‐110‐21121‐01‐N‐01), and Taipei Medical University (TMU107‐AE1‐B15, 107‐3805‐003‐110, 107TMU‐SHH‐03). The open‐access publication of this article was funded by the Priority Research Area BioS under the program "Excellence Initiative – Research University" at the Jagiellonian University in Krakow. 1 2
Publisher Copyright:
© 2021 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.
PY - 2021/10
Y1 - 2021/10
N2 - The orexinergic system delivers excitation for multiple brain centers to facilitate behavioral arousal, with its malfunction resulting in narcolepsy, somnolence, and notably, visual hallucinations. Since the circadian clock underlies the daily arousal, a timed coordination is expected between the orexin system and its target subcortical visual system, including the superior colliculus (SC). Here, we use a combination of electrophysiological, immunohistochemical, and molecular approaches across 24 h, together with the neuronal tract-tracing methods to investigate the daily coordination between the orexin system and the rodent SC. Higher orexinergic input was found to occur nocturnally in the superficial layers of the SC, in time for nocturnal silencing of spontaneous firing in this visual brain area. We identify autonomous daily and circadian expression of clock genes in the SC, which may underlie these day–night changes. Additionally, we establish the lateral hypothalamic origin of the orexin innervation to the SC and that the SC neurons robustly respond to orexin A via OX2 receptor in both excitatory and GABAA receptor-dependent inhibitory manners. Together, our evidence elucidates the combination of intrinsic and extrinsic clock mechanisms that shape the daily function of the visual layers of the SC.
AB - The orexinergic system delivers excitation for multiple brain centers to facilitate behavioral arousal, with its malfunction resulting in narcolepsy, somnolence, and notably, visual hallucinations. Since the circadian clock underlies the daily arousal, a timed coordination is expected between the orexin system and its target subcortical visual system, including the superior colliculus (SC). Here, we use a combination of electrophysiological, immunohistochemical, and molecular approaches across 24 h, together with the neuronal tract-tracing methods to investigate the daily coordination between the orexin system and the rodent SC. Higher orexinergic input was found to occur nocturnally in the superficial layers of the SC, in time for nocturnal silencing of spontaneous firing in this visual brain area. We identify autonomous daily and circadian expression of clock genes in the SC, which may underlie these day–night changes. Additionally, we establish the lateral hypothalamic origin of the orexin innervation to the SC and that the SC neurons robustly respond to orexin A via OX2 receptor in both excitatory and GABAA receptor-dependent inhibitory manners. Together, our evidence elucidates the combination of intrinsic and extrinsic clock mechanisms that shape the daily function of the visual layers of the SC.
KW - circadian clock
KW - multi-electrode array
KW - orexin
KW - superior colliculus
KW - visual system
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U2 - 10.1096/fj.202100779RR
DO - 10.1096/fj.202100779RR
M3 - Article
AN - SCOPUS:85115880652
SN - 0892-6638
VL - 35
JO - FASEB Journal
JF - FASEB Journal
IS - 10
M1 - e21930
ER -