Cytokine-induced killer T cells enhance the cytotoxicity against carboplatin-resistant ovarian cancer cells

Yueh Pan; Ya Hsu Chiu; Shao Chih Chiu; Der Yang Cho; Liang Ming Lee; Yu Ching Wen; Jacqueline Whang-Peng; Chi Hao Hsiao; Ping Hsiao Shih

doi:10.21873/ANTICANRES.14376

Cytokine-induced killer T cells enhance the cytotoxicity against carboplatin-resistant ovarian cancer cells

Yueh Pan, Ya Hsu Chiu, Shao Chih Chiu, Der Yang Cho, Liang Ming Lee, Yu Ching Wen, Jacqueline Whang-Peng, Chi Hao Hsiao, Ping Hsiao Shih

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Background/Aim: Ovarian cancer (OC) is typically diagnosed at an advanced stage with limitations for cure. Cytokine-induced killer (CIK) T cell therapy exerts significant cytotoxic effects against cancer cells and reduces the adverse effects of chemotherapy. Herein, we performed a flow cytometry-based method to evaluate the cytotoxicity of peripheral blood mononuclear cells-derived CIK cells against OC cells. Materials and Methods: The CIK cells were induced and expanded using an interferon-γ/IL-2-based xeno-free medium system. The cytotoxicity of CIK cells or carboplatin against OC cells was examined. Results: The CIK cells showed an NK-like phenotypic characteristic and dose-dependently increased cytotoxicity against OC cells. We found that the number of advanced OC cells, which were more resistant to carboplatin, was dramatically decreased by an additional one-shot CIK treatment. Conclusion: CIK cells have a potent cytotoxic ability that would be explored as an alternative strategy for cancer treatment in the near future.

Original language	English
Pages (from-to)	3865-3872
Number of pages	8
Journal	Anticancer Research
Volume	40
Issue number	7
DOIs	https://doi.org/10.21873/ANTICANRES.14376
Publication status	Published - Jul 2020

Keywords

Chemotherapy
Cytokine-induced killer cell
Flow cytometry
Ovarian cancer
Peripheral blood mononuclear cell

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.21873/ANTICANRES.14376

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title = "Cytokine-induced killer T cells enhance the cytotoxicity against carboplatin-resistant ovarian cancer cells",

abstract = "Background/Aim: Ovarian cancer (OC) is typically diagnosed at an advanced stage with limitations for cure. Cytokine-induced killer (CIK) T cell therapy exerts significant cytotoxic effects against cancer cells and reduces the adverse effects of chemotherapy. Herein, we performed a flow cytometry-based method to evaluate the cytotoxicity of peripheral blood mononuclear cells-derived CIK cells against OC cells. Materials and Methods: The CIK cells were induced and expanded using an interferon-γ/IL-2-based xeno-free medium system. The cytotoxicity of CIK cells or carboplatin against OC cells was examined. Results: The CIK cells showed an NK-like phenotypic characteristic and dose-dependently increased cytotoxicity against OC cells. We found that the number of advanced OC cells, which were more resistant to carboplatin, was dramatically decreased by an additional one-shot CIK treatment. Conclusion: CIK cells have a potent cytotoxic ability that would be explored as an alternative strategy for cancer treatment in the near future.",

keywords = "Chemotherapy, Cytokine-induced killer cell, Flow cytometry, Ovarian cancer, Peripheral blood mononuclear cell",

author = "Yueh Pan and Chiu, {Ya Hsu} and Chiu, {Shao Chih} and Cho, {Der Yang} and Lee, {Liang Ming} and Wen, {Yu Ching} and Jacqueline Whang-Peng and Hsiao, {Chi Hao} and Shih, {Ping Hsiao}",

year = "2020",

month = jul,

doi = "10.21873/ANTICANRES.14376",

language = "English",

volume = "40",

pages = "3865--3872",

journal = "Anticancer Research",

issn = "0250-7005",

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TY - JOUR

T1 - Cytokine-induced killer T cells enhance the cytotoxicity against carboplatin-resistant ovarian cancer cells

AU - Pan, Yueh

AU - Chiu, Ya Hsu

AU - Chiu, Shao Chih

AU - Cho, Der Yang

AU - Lee, Liang Ming

AU - Wen, Yu Ching

AU - Whang-Peng, Jacqueline

AU - Hsiao, Chi Hao

AU - Shih, Ping Hsiao

PY - 2020/7

Y1 - 2020/7

N2 - Background/Aim: Ovarian cancer (OC) is typically diagnosed at an advanced stage with limitations for cure. Cytokine-induced killer (CIK) T cell therapy exerts significant cytotoxic effects against cancer cells and reduces the adverse effects of chemotherapy. Herein, we performed a flow cytometry-based method to evaluate the cytotoxicity of peripheral blood mononuclear cells-derived CIK cells against OC cells. Materials and Methods: The CIK cells were induced and expanded using an interferon-γ/IL-2-based xeno-free medium system. The cytotoxicity of CIK cells or carboplatin against OC cells was examined. Results: The CIK cells showed an NK-like phenotypic characteristic and dose-dependently increased cytotoxicity against OC cells. We found that the number of advanced OC cells, which were more resistant to carboplatin, was dramatically decreased by an additional one-shot CIK treatment. Conclusion: CIK cells have a potent cytotoxic ability that would be explored as an alternative strategy for cancer treatment in the near future.

AB - Background/Aim: Ovarian cancer (OC) is typically diagnosed at an advanced stage with limitations for cure. Cytokine-induced killer (CIK) T cell therapy exerts significant cytotoxic effects against cancer cells and reduces the adverse effects of chemotherapy. Herein, we performed a flow cytometry-based method to evaluate the cytotoxicity of peripheral blood mononuclear cells-derived CIK cells against OC cells. Materials and Methods: The CIK cells were induced and expanded using an interferon-γ/IL-2-based xeno-free medium system. The cytotoxicity of CIK cells or carboplatin against OC cells was examined. Results: The CIK cells showed an NK-like phenotypic characteristic and dose-dependently increased cytotoxicity against OC cells. We found that the number of advanced OC cells, which were more resistant to carboplatin, was dramatically decreased by an additional one-shot CIK treatment. Conclusion: CIK cells have a potent cytotoxic ability that would be explored as an alternative strategy for cancer treatment in the near future.

KW - Chemotherapy

KW - Cytokine-induced killer cell

KW - Flow cytometry

KW - Ovarian cancer

KW - Peripheral blood mononuclear cell

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Cytokine-induced killer T cells enhance the cytotoxicity against carboplatin-resistant ovarian cancer cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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