Cytogenetic studies of lymphoma cells from an american patient with a tumor similar to burkitt's tumors in african children

Cytogenetic studies were done on lymphoma cells from ascitic fluid of an American woman with a tumor similar to Burkitt's tumors in African children and on the established cell culture line (AL-2) derived from the ascites cells, More than 90% of the metaphases in direct preparations of the ascitic fluid contained an extra submedium chromosome, larger than pair #1, and lacked one chromosome from this #1 pair. The AL-2 line had a chromosome number distribution ranging from 42–86 with a modal number of 46, and the cells with 46 chromosomes had an apparently normal female karyotype. None of the AL-2 cells showed any marker chromosomes. The cultural conditions apparently favored the growth of those cells without the marker chromosome.