TY - JOUR
T1 - Cucurbitane-type glycosides from the fruits of Momordica charantia and their hypoglycaemic and cytotoxic activities
AU - Zhang, Li Jie
AU - Liaw, Chia Ching
AU - Hsiao, Ping Chun
AU - Huang, Hui Chi
AU - Lin, Ming Jen
AU - Lin, Zhi Hu
AU - Hsu, Feng Lin
AU - Kuo, Yao Haur
N1 - Funding Information:
The authors thank the National Research Institute of Chinese Medicine, Taipei, Taiwan for financial support, and Starsci Biotech Co. Ltd., Taipei, Taiwan for providing the fruits of M. charantia and experimental assistance.
PY - 2014
Y1 - 2014
N2 - Eight new cucurbitane-type glycosides, kuguasaponins A-H (1-8), and six known compounds (9-14), were isolated by the bioassay-directed fractionation of the fruits of Momordica charantia. The structures of the compounds were established via spectroscopic analyses, including NMR, IR, and MS techniques. The stereochemistry of the isolated cucurbitanes was further determined using X-ray crystallographic analysis, NOESY experiments, and acid hydrolysis, as well as comparison with the cucurbitanes reported in the literature. The HPLC profiles of the active fraction by an ELSD detector were established and used to identify the 7 main peaks of the isolated cucurbitanes. Pharmacological studies on the anti-hyperglycaemic effects revealed that compounds 2, 3, 7, 8, and 13 exhibited strong bioactivities at 10. μM based on the glucose uptake assay. In addition, compounds 2-5, and 14 exhibited moderate cytotoxicity against MCF-7, Doay, HEp-2, and WiDr human tumour cell lines and no activity against the M10 cell line.
AB - Eight new cucurbitane-type glycosides, kuguasaponins A-H (1-8), and six known compounds (9-14), were isolated by the bioassay-directed fractionation of the fruits of Momordica charantia. The structures of the compounds were established via spectroscopic analyses, including NMR, IR, and MS techniques. The stereochemistry of the isolated cucurbitanes was further determined using X-ray crystallographic analysis, NOESY experiments, and acid hydrolysis, as well as comparison with the cucurbitanes reported in the literature. The HPLC profiles of the active fraction by an ELSD detector were established and used to identify the 7 main peaks of the isolated cucurbitanes. Pharmacological studies on the anti-hyperglycaemic effects revealed that compounds 2, 3, 7, 8, and 13 exhibited strong bioactivities at 10. μM based on the glucose uptake assay. In addition, compounds 2-5, and 14 exhibited moderate cytotoxicity against MCF-7, Doay, HEp-2, and WiDr human tumour cell lines and no activity against the M10 cell line.
KW - Cucurbitane
KW - Cytotoxicity
KW - Hypoglycaemic activity
KW - Kuguasaponins
KW - Momordica charantia
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U2 - 10.1016/j.jff.2013.11.025
DO - 10.1016/j.jff.2013.11.025
M3 - Article
AN - SCOPUS:84895071464
SN - 1756-4646
VL - 6
SP - 564
EP - 574
JO - Journal of Functional Foods
JF - Journal of Functional Foods
IS - 1
ER -