CTCs detection by SACA chip and image analysis

Wanying Cho; Yi Hsuan Weng; Hsin Ying Chen; Hsueh Yao Chu; Tzeon Jye Chiou; Chien Ping Lin; Jeng Kai Jiang; Chih Yung Yang; Fan Gang Tseng

doi:10.1109/NEMS.2017.8017042

CTCs detection by SACA chip and image analysis

Wanying Cho, Yi Hsuan Weng, Hsin Ying Chen, Hsueh Yao Chu, Tzeon Jye Chiou, Chien Ping Lin, Jeng Kai Jiang, Chih Yung Yang, Fan Gang Tseng

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Citations (Scopus)

Abstract

Immunofluorescence is widely used in detection and characterization of circulating tumor cells (CTCs). The process of immunofluorescence staining on cells needs centrifugal force to wash residual fluorescence, which is not only time consuming but also cells harmful. To speed up the staining process, a three-dimensional (3D) microfluidic device is employed in this work. This study also reports the rapid selection of identified live CTCs from whole blood on SACA chip for further characterization by automatic imaging and in-situ cell capturing process. The whole process takes 120 minutes for examining 4 ml whole blood sample and the recognition sensitivity can approach 1/108 by using the integrated chip and automatic image system. All cells are remained highly viable after the rapid selection process for further applications.

Original language	English
Title of host publication	2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	360-363
Number of pages	4
ISBN (Electronic)	9781509030590
DOIs	https://doi.org/10.1109/NEMS.2017.8017042
Publication status	Published - Aug 25 2017
Externally published	Yes
Event	12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 - Los Angeles, United States Duration: Apr 9 2017 → Apr 12 2017

Publication series

Name	2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

Other

Other	12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
Country/Territory	United States
City	Los Angeles
Period	4/9/17 → 4/12/17

Keywords

automatic detection
circulating tumor cell
SACA chip

ASJC Scopus subject areas

Electrical and Electronic Engineering
Mechanical Engineering
Electronic, Optical and Magnetic Materials
Instrumentation

Access to Document

10.1109/NEMS.2017.8017042

Cite this

Cho, W., Weng, Y. H., Chen, H. Y., Chu, H. Y., Chiou, T. J., Lin, C. P., Jiang, J. K., Yang, C. Y., & Tseng, F. G. (2017). CTCs detection by SACA chip and image analysis. In 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 (pp. 360-363). Article 8017042 (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2017.8017042

CTCs detection by SACA chip and image analysis. / Cho, Wanying; Weng, Yi Hsuan; Chen, Hsin Ying et al.
2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. p. 360-363 8017042 (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Cho, W, Weng, YH, Chen, HY, Chu, HY, Chiou, TJ, Lin, CP, Jiang, JK, Yang, CY & Tseng, FG 2017, CTCs detection by SACA chip and image analysis. in 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017., 8017042, 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017, Institute of Electrical and Electronics Engineers Inc., pp. 360-363, 12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017, Los Angeles, United States, 4/9/17. https://doi.org/10.1109/NEMS.2017.8017042

Cho W, Weng YH, Chen HY, Chu HY, Chiou TJ, Lin CP et al. CTCs detection by SACA chip and image analysis. In 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017. Institute of Electrical and Electronics Engineers Inc. 2017. p. 360-363. 8017042. (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017). doi: 10.1109/NEMS.2017.8017042

Cho, Wanying ; Weng, Yi Hsuan ; Chen, Hsin Ying et al. / CTCs detection by SACA chip and image analysis. 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 360-363 (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017).

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title = "CTCs detection by SACA chip and image analysis",

abstract = "Immunofluorescence is widely used in detection and characterization of circulating tumor cells (CTCs). The process of immunofluorescence staining on cells needs centrifugal force to wash residual fluorescence, which is not only time consuming but also cells harmful. To speed up the staining process, a three-dimensional (3D) microfluidic device is employed in this work. This study also reports the rapid selection of identified live CTCs from whole blood on SACA chip for further characterization by automatic imaging and in-situ cell capturing process. The whole process takes 120 minutes for examining 4 ml whole blood sample and the recognition sensitivity can approach 1/108 by using the integrated chip and automatic image system. All cells are remained highly viable after the rapid selection process for further applications.",

keywords = "automatic detection, circulating tumor cell, SACA chip",

author = "Wanying Cho and Weng, {Yi Hsuan} and Chen, {Hsin Ying} and Chu, {Hsueh Yao} and Chiou, {Tzeon Jye} and Lin, {Chien Ping} and Jiang, {Jeng Kai} and Yang, {Chih Yung} and Tseng, {Fan Gang}",

note = "Funding Information: ACKNOWLEDGMENT This research was financially supported by the Ministry of Science and Technology of the Republic of China. Publisher Copyright: {\textcopyright} 2017 IEEE.; 12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 ; Conference date: 09-04-2017 Through 12-04-2017",

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doi = "10.1109/NEMS.2017.8017042",

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AU - Cho, Wanying

AU - Weng, Yi Hsuan

AU - Chen, Hsin Ying

AU - Chu, Hsueh Yao

AU - Chiou, Tzeon Jye

AU - Lin, Chien Ping

AU - Jiang, Jeng Kai

AU - Yang, Chih Yung

AU - Tseng, Fan Gang

PY - 2017/8/25

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N2 - Immunofluorescence is widely used in detection and characterization of circulating tumor cells (CTCs). The process of immunofluorescence staining on cells needs centrifugal force to wash residual fluorescence, which is not only time consuming but also cells harmful. To speed up the staining process, a three-dimensional (3D) microfluidic device is employed in this work. This study also reports the rapid selection of identified live CTCs from whole blood on SACA chip for further characterization by automatic imaging and in-situ cell capturing process. The whole process takes 120 minutes for examining 4 ml whole blood sample and the recognition sensitivity can approach 1/108 by using the integrated chip and automatic image system. All cells are remained highly viable after the rapid selection process for further applications.

AB - Immunofluorescence is widely used in detection and characterization of circulating tumor cells (CTCs). The process of immunofluorescence staining on cells needs centrifugal force to wash residual fluorescence, which is not only time consuming but also cells harmful. To speed up the staining process, a three-dimensional (3D) microfluidic device is employed in this work. This study also reports the rapid selection of identified live CTCs from whole blood on SACA chip for further characterization by automatic imaging and in-situ cell capturing process. The whole process takes 120 minutes for examining 4 ml whole blood sample and the recognition sensitivity can approach 1/108 by using the integrated chip and automatic image system. All cells are remained highly viable after the rapid selection process for further applications.

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CTCs detection by SACA chip and image analysis

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Keywords

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