TY - JOUR
T1 - Controlled Attachment of Pseudomonas aeruginosa with Binary Colloidal Crystal-Based Topographies
AU - Pingle, Hitesh
AU - Wang, Peng Yuan
AU - Thissen, Helmut
AU - Kingshott, Peter
N1 - Funding Information:
The Australian Research Council (ARC) is acknowledged for funding a PhD scholarship for H.P. through a Discovery Project, and for funding P.Y.W. through a Discovery Early Career Researcher Award (ARC-DECRA). The Scientific Industrial Endowment Fund (SIEF) is also acknowledged for providing Postdoctoral Research Fellowship for P.Y.W. This work was supported in part at both the Biointerface Engineering Hub at Swinburne and the Melbourne Centre for Nanofabrication as part of the Victorian Node of the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano-and microfabrication facilities for Australia’s researchers.
Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2018/4/5
Y1 - 2018/4/5
N2 - Micro- and nanotopographies can interfere with bacteria attachment, however, the interplay existing between surface chemistry and topography remains unclear. Here, self-assembled spherical micrometer- silica and nanometer poly(methyl methacrylate) (PMMA)-sized particles are used to make binary colloidal crystal (BCC) topographical patterns to study bacterial attachment. A uniform surface chemistry of allylamine plasma polymer (AAMpp) is coated on the top of the BCCs to study only the topography effects. The uncoated and coated BCCs are exposed to Pseudomonas aeruginosa, and the surfaces and bacteria are characterized using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and fluorescence microscopy. It is found that bacteria attachment to the uncoated BCCs is delayed and individual cells are attracted to the small particle regions of the patterns. Surprisingly, this phenomenon is also observed for the AAMpp-coated BCCs, with bacteria attaching to the small particle regions of the pattern, in stark contrast to uniform flat films of AAMpp that are highly adhesive toward P. aeruginosa. Also, the overall levels of bacterial attachment are significantly reduced by the BCC patterns compared to controls. Thus, there is a trade-off that exists between chemistry and topography that can be exploited to delay the onset of P. aeruginosa biofilm formation on surfaces.
AB - Micro- and nanotopographies can interfere with bacteria attachment, however, the interplay existing between surface chemistry and topography remains unclear. Here, self-assembled spherical micrometer- silica and nanometer poly(methyl methacrylate) (PMMA)-sized particles are used to make binary colloidal crystal (BCC) topographical patterns to study bacterial attachment. A uniform surface chemistry of allylamine plasma polymer (AAMpp) is coated on the top of the BCCs to study only the topography effects. The uncoated and coated BCCs are exposed to Pseudomonas aeruginosa, and the surfaces and bacteria are characterized using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and fluorescence microscopy. It is found that bacteria attachment to the uncoated BCCs is delayed and individual cells are attracted to the small particle regions of the patterns. Surprisingly, this phenomenon is also observed for the AAMpp-coated BCCs, with bacteria attaching to the small particle regions of the pattern, in stark contrast to uniform flat films of AAMpp that are highly adhesive toward P. aeruginosa. Also, the overall levels of bacterial attachment are significantly reduced by the BCC patterns compared to controls. Thus, there is a trade-off that exists between chemistry and topography that can be exploited to delay the onset of P. aeruginosa biofilm formation on surfaces.
KW - Binary colloidal crystals
KW - Biofilms
KW - Plasma polymerisation
KW - Pseudomonas aeruginosa
KW - Surface modification
KW - surface modification
KW - binary colloidal crystals
KW - biofilms
KW - plasma polymerisation
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U2 - 10.1002/smll.201703574
DO - 10.1002/smll.201703574
M3 - Article
AN - SCOPUS:85042536966
SN - 1613-6810
VL - 14
JO - Small
JF - Small
IS - 14
M1 - 1703574
ER -