Continuous cell line from pupal ovary of Perina nuda (Lepidoptera: Lymantriidae) that is permissive to nuclear polyhedrosis virus from P. nuda

Chung Hsiung Wang; Chih Ming Chou; Hwei Chung Liu; Shih Liang Kau; Guang Hsung Kou; Chu Fang Lo

doi:10.1006/jipa.1996.0033

Continuous cell line from pupal ovary of Perina nuda (Lepidoptera: Lymantriidae) that is permissive to nuclear polyhedrosis virus from P. nuda

Chung Hsiung Wang, Chih Ming Chou, Hwei Chung Liu, Shih Liang Kau, Guang Hsung Kou, Chu Fang Lo

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

A continuous cell line, designated NTU-PN-HH, was established from the pupal ovary of Perina nuda Fabricius (Lepidoptera: Lymantriidae). The cells have been through more than 300 passages during 4 years in TNM-FH medium supplemented with 10% FBS, at a constant temperature of 28°C. The cell line consists of four major morphologic types: polymorphic cells, spindle-shaped cells, round cells, and squamous cells. The characterization of this cell line showed that NTU-PN-HH is a newly established cell line. It is the first cell line that is permissive to PnNPV (P. nuda multiple nuclear polyhedrosis virus).

Original language	English
Pages (from-to)	199-204
Number of pages	6
Journal	Journal of Invertebrate Pathology
Volume	67
Issue number	3
DOIs	https://doi.org/10.1006/jipa.1996.0033
Publication status	Published - May 1996
Externally published	Yes

Keywords

NTU-PN-HH cell line
Perina nuda
PnNPV

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Insect Science

Access to Document

10.1006/jipa.1996.0033

Cite this

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title = "Continuous cell line from pupal ovary of Perina nuda (Lepidoptera: Lymantriidae) that is permissive to nuclear polyhedrosis virus from P. nuda",

abstract = "A continuous cell line, designated NTU-PN-HH, was established from the pupal ovary of Perina nuda Fabricius (Lepidoptera: Lymantriidae). The cells have been through more than 300 passages during 4 years in TNM-FH medium supplemented with 10% FBS, at a constant temperature of 28°C. The cell line consists of four major morphologic types: polymorphic cells, spindle-shaped cells, round cells, and squamous cells. The characterization of this cell line showed that NTU-PN-HH is a newly established cell line. It is the first cell line that is permissive to PnNPV (P. nuda multiple nuclear polyhedrosis virus).",

keywords = "NTU-PN-HH cell line, Perina nuda, PnNPV",

author = "Wang, {Chung Hsiung} and Chou, {Chih Ming} and Liu, {Hwei Chung} and Kau, {Shih Liang} and Kou, {Guang Hsung} and Lo, {Chu Fang}",

year = "1996",

month = may,

doi = "10.1006/jipa.1996.0033",

language = "English",

volume = "67",

pages = "199--204",

journal = "Journal of Invertebrate Pathology",

issn = "0022-2011",

publisher = "Academic Press Inc.",

number = "3",

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TY - JOUR

T1 - Continuous cell line from pupal ovary of Perina nuda (Lepidoptera

T2 - Lymantriidae) that is permissive to nuclear polyhedrosis virus from P. nuda

AU - Wang, Chung Hsiung

AU - Chou, Chih Ming

AU - Liu, Hwei Chung

AU - Kau, Shih Liang

AU - Kou, Guang Hsung

AU - Lo, Chu Fang

PY - 1996/5

Y1 - 1996/5

N2 - A continuous cell line, designated NTU-PN-HH, was established from the pupal ovary of Perina nuda Fabricius (Lepidoptera: Lymantriidae). The cells have been through more than 300 passages during 4 years in TNM-FH medium supplemented with 10% FBS, at a constant temperature of 28°C. The cell line consists of four major morphologic types: polymorphic cells, spindle-shaped cells, round cells, and squamous cells. The characterization of this cell line showed that NTU-PN-HH is a newly established cell line. It is the first cell line that is permissive to PnNPV (P. nuda multiple nuclear polyhedrosis virus).

AB - A continuous cell line, designated NTU-PN-HH, was established from the pupal ovary of Perina nuda Fabricius (Lepidoptera: Lymantriidae). The cells have been through more than 300 passages during 4 years in TNM-FH medium supplemented with 10% FBS, at a constant temperature of 28°C. The cell line consists of four major morphologic types: polymorphic cells, spindle-shaped cells, round cells, and squamous cells. The characterization of this cell line showed that NTU-PN-HH is a newly established cell line. It is the first cell line that is permissive to PnNPV (P. nuda multiple nuclear polyhedrosis virus).

KW - NTU-PN-HH cell line

KW - Perina nuda

KW - PnNPV

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Continuous cell line from pupal ovary of Perina nuda (Lepidoptera: Lymantriidae) that is permissive to nuclear polyhedrosis virus from P. nuda

Abstract

Keywords

ASJC Scopus subject areas

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