TY - GEN
T1 - Construct logic operation gates of OR and and with multiple enzymes
AU - Yuyi, Chu
AU - Wu, Juiyu
AU - Watada, Junzo
AU - Kim, Ikno
PY - 2010
Y1 - 2010
N2 - Biomoleculars act as a tool to build a multiple enzyme system based on human metabolic actions to perform basic logic operation connecting OR and AND gates. Three enzymes (invertase, amyloglucosidase, hexokinase) concert as a logic operation part, processing three molecular input signals (sucrose, maltose, and ATP) to produce G6P (glucose-6-phosphate). The other two enzymes glucose-6-phosphate dehydrogenase (G6PD) and salicylate hydroxylase (SHL) compose to function as a signal displayer. Furthermore, we used a latent fluorescent molecule composed of sacylate and fluorescence which can be catalyzed and release fluorescent molecular to produce output signal. According to our experiments, firstly, our design is proved. The typical characteristics of enzyme reactions have been discovered through comparing the expected theoretical cures with the result cures. Secondly, the possibility of applying multiple logic gates into complicate networks has been shown.
AB - Biomoleculars act as a tool to build a multiple enzyme system based on human metabolic actions to perform basic logic operation connecting OR and AND gates. Three enzymes (invertase, amyloglucosidase, hexokinase) concert as a logic operation part, processing three molecular input signals (sucrose, maltose, and ATP) to produce G6P (glucose-6-phosphate). The other two enzymes glucose-6-phosphate dehydrogenase (G6PD) and salicylate hydroxylase (SHL) compose to function as a signal displayer. Furthermore, we used a latent fluorescent molecule composed of sacylate and fluorescence which can be catalyzed and release fluorescent molecular to produce output signal. According to our experiments, firstly, our design is proved. The typical characteristics of enzyme reactions have been discovered through comparing the expected theoretical cures with the result cures. Secondly, the possibility of applying multiple logic gates into complicate networks has been shown.
KW - Bio-computing
KW - Boolean logic gates
KW - Enzymes
KW - Latent fluorophore
UR - http://www.scopus.com/inward/record.url?scp=79952563609&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79952563609&partnerID=8YFLogxK
U2 - 10.1109/ICGEC.2010.17
DO - 10.1109/ICGEC.2010.17
M3 - Conference contribution
AN - SCOPUS:79952563609
SN - 9780769542812
T3 - Proceedings - 4th International Conference on Genetic and Evolutionary Computing, ICGEC 2010
SP - 35
EP - 38
BT - Proceedings - 4th International Conference on Genetic and Evolutionary Computing, ICGEC 2010
T2 - 4th International Conference on Genetic and Evolutionary Computing, ICGEC 2010
Y2 - 13 December 2010 through 15 December 2010
ER -