Congenital adrenal hyperplasia: Molecular characterization

Tsang Ming Ko, Chuan Hong Kao, Hong Nerng Ho, Li Hui Tseng, Hsiao Lin Hwa, Pi Mei Hsu, Sou Ming Chuang, Tzu Yao Lee

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


OBJECTIVE: To study the molecular defects of congenital adrenal hyperplasia (CAH). STUDY DESIGN: Twenty Chinese patients, including 8 with salt-wasting (SW) type CAH, 11 with simple virilizing (SV) type CAH and 1 with nonclassical (NC) type CAH, were recruited. Two rounds of the polymerase chain reaction (PCR) were used to study the 21-hydroxylase gene (CYP21). The primary PCR amplified CYP21-specific DNA fragments, and the secondary PCR used products from the primary PCR for analysis of amplification-created restriction sites (ACRS) and direct DNA sequencing. In all patients, ACRS analysis was done at 12 possible mutation sites, and then direct DNA sequencing was performed to confirm or define the molecular defects. RESULTS: Ten different mutations, including nine point mutations and gross gene deletion or conversion, were found in this study. Of the nine point mutations, eight could be easily detected by ACRS analysis. The three most common mutations were codon (CD)172 t→a (I172N), IVS-II 656 c/a→g, and gross gene deletion or conversion, accounting for 27.5% (11/40 alleles), 25% (10/40) and 20% (8/40) of all identified mutations, respectively. All SW patients were compound heterozygotes of IVS-II 656, gross gene deletion or conversion, or other severe defects, including CDs236 (t→a) (I236N) + 237 (t→a) (V237E) + 239 (t→a) (M239K), CD306 (+ t), CD318 (c→t) (Q318X) and CD356 (c→t) (R356W) mutations. All SV patients had one allele with a CD172 (I172N) mutation. One allele of an NC patient had a CD183 (c→g) (D183E) mutation, and the other allele was not defined. In the whole series, four alleles (10%) had more than one mutation. CONCLUSION: We found 10 different mutations in this study. The correlation between genotypes and phenotypes was compatible with the reported data. Two rounds of PCR and ACRS analysis may provide important in formation for genetic counseling, prenatal diagnosis and management of families at risk for CAH.

Original languageEnglish
Pages (from-to)379-386
Number of pages8
JournalJournal of Reproductive Medicine for the Obstetrician and Gynecologist
Issue number4
Publication statusPublished - Apr 1 1998
Externally publishedYes


  • Adrenal hyperplasia, congenital
  • DNA
  • Gene amplification

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine


Dive into the research topics of 'Congenital adrenal hyperplasia: Molecular characterization'. Together they form a unique fingerprint.

Cite this