Abstract
The structures of the (C-T)n sequence at two different pHs have been analyzed by 500 MHz 2D-NMR using a modified DNA decamer d(CT[m5C]TCU[m5C]UCT) as a model system. The chemical modifications serve to perturb the monotonous C-T repeat, and consequently to yield a better chemical shift dispersion. The results reinforce our earlier suggestion that there are three major pH-depedent confonnational species: two antiparallel-stranded (APS) duplexes at pH 7 and pH 3, and a different structure near pH 5. Structural refinement of the decamer duplexes at pH = 7.5 and pH = 2.9 using 2D-NOE data suggests that the C : T or C+ : T base pairs are continuously stacked. Exchangeable proton NMR spectra at pH 7.5 and pH 2.9 are consistent with C : T or C+ : T base pairing schemes in which a water molecule bridges the two bases.
Original language | English |
---|---|
Pages (from-to) | 139-144 |
Number of pages | 6 |
Journal | FEBS Letters |
Volume | 337 |
Issue number | 2 |
DOIs | |
Publication status | Published - Jan 10 1994 |
Externally published | Yes |
Keywords
- Modified DNA base
- Nuclear Overhauser effect
- Unusual DNA conformation
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology