Computational Analysis of Antibody Paratopes for Antibody Sequences in Antibody Libraries

Hung Pin Peng, An Suei Yang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

To ensure the functionalities of the antibodies in phage-displayed synthetic antibody libraries, we use computational method to evaluate the designs of the antibody libraries. The computational methodologies developed in our lab for designing antibody library provide rich information on the function of the designed antibody sequences—adequate antibody designs for a specific antigen type should have predicted paratopes for the antigen type. This computational assessment of the designed antibody sequences helps eliminate non-functional designs before proceeding to construct the library designs in the wet lab. As such, only reasonable antibody designs are constructed for antibody discoveries.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages437-445
Number of pages9
DOIs
Publication statusPublished - 2023
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2552
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Antibody modeling
  • Antibody-protein interactions
  • Functional paratope
  • Phage display
  • Rational antibody design
  • Synthetic antibody library

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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