Complete microscale profiling of tumor microangiogenesis. A microradiological methodology reveals fundamental aspects of tumor angiogenesis and yields an array of quantitative parameters for its characterization.

Chia Chi Chien; Ivan M. Kempson; Cheng Liang Wang; Hsianghisn Chen; Yeukuang Hwu; Y. N. Chen; Ting-Kuo Lee; Kelvin K C Tsai; Ming Sheng Liu; Kwang Yu Chang; Chung Shi Yang; G. Margaritondo

doi:10.1016/j.biotechadv.2011.12.001

Complete microscale profiling of tumor microangiogenesis. A microradiological methodology reveals fundamental aspects of tumor angiogenesis and yields an array of quantitative parameters for its characterization.

Chia Chi Chien, Ivan M. Kempson, Cheng Liang Wang, Hsianghisn Chen, Yeukuang Hwu, Y. N. Chen, Ting-Kuo Lee, Kelvin K C Tsai, Ming Sheng Liu, Kwang Yu Chang, Chung Shi Yang, G. Margaritondo

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Complete profiling would substantially facilitate the fundamental understanding of tumor angiogenesis and of possible anti-angiogenesis cancer treatments. We developed an integrated synchrotron-based methodology with excellent performances: detection of very small vessels by high spatial resolution (~. 1. μm) and nanoparticle contrast enhancement, in vivo dynamics investigations with high temporal resolution (~. 1. ms), and three-dimensional quantitative morphology parametrization by computer tracing. The smallest (3-10. μm) microvessels were found to constitute >80% of the tumor vasculature and exhibit many structural anomalies. Practical applications are presented, including vessel microanalysis in xenografted tumors, monitoring the effects of anti-angiogenetic agents and in vivo detection of tumor vascular rheological properties.

Original language	English
Pages (from-to)	396-401
Number of pages	6
Journal	Biotechnology Advances
Volume	31
Issue number	3
DOIs	https://doi.org/10.1016/j.biotechadv.2011.12.001
Publication status	Published - May 2013
Externally published	Yes

Keywords

Angiogenesis
Quantitative analysis
Solid tumor
Synchrotron
X-ray imaging

ASJC Scopus subject areas

Biotechnology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biotechadv.2011.12.001

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Chien, C. C., Kempson, I. M., Wang, C. L., Chen, H., Hwu, Y., Chen, Y. N., Lee, T.-K., Tsai, K. K. C., Liu, M. S., Chang, K. Y., Yang, C. S., & Margaritondo, G. (2013). Complete microscale profiling of tumor microangiogenesis. A microradiological methodology reveals fundamental aspects of tumor angiogenesis and yields an array of quantitative parameters for its characterization. Biotechnology Advances, 31(3), 396-401. https://doi.org/10.1016/j.biotechadv.2011.12.001

Complete microscale profiling of tumor microangiogenesis. A microradiological methodology reveals fundamental aspects of tumor angiogenesis and yields an array of quantitative parameters for its characterization. / Chien, Chia Chi; Kempson, Ivan M.; Wang, Cheng Liang et al.
In: Biotechnology Advances, Vol. 31, No. 3, 05.2013, p. 396-401.

Research output: Contribution to journal › Article › peer-review

Chien, CC, Kempson, IM, Wang, CL, Chen, H, Hwu, Y, Chen, YN, Lee, T-K, Tsai, KKC, Liu, MS, Chang, KY, Yang, CS & Margaritondo, G 2013, 'Complete microscale profiling of tumor microangiogenesis. A microradiological methodology reveals fundamental aspects of tumor angiogenesis and yields an array of quantitative parameters for its characterization.', Biotechnology Advances, vol. 31, no. 3, pp. 396-401. https://doi.org/10.1016/j.biotechadv.2011.12.001

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title = "Complete microscale profiling of tumor microangiogenesis. A microradiological methodology reveals fundamental aspects of tumor angiogenesis and yields an array of quantitative parameters for its characterization.",

abstract = "Complete profiling would substantially facilitate the fundamental understanding of tumor angiogenesis and of possible anti-angiogenesis cancer treatments. We developed an integrated synchrotron-based methodology with excellent performances: detection of very small vessels by high spatial resolution (~. 1. μm) and nanoparticle contrast enhancement, in vivo dynamics investigations with high temporal resolution (~. 1. ms), and three-dimensional quantitative morphology parametrization by computer tracing. The smallest (3-10. μm) microvessels were found to constitute >80% of the tumor vasculature and exhibit many structural anomalies. Practical applications are presented, including vessel microanalysis in xenografted tumors, monitoring the effects of anti-angiogenetic agents and in vivo detection of tumor vascular rheological properties.",

keywords = "Angiogenesis, Quantitative analysis, Solid tumor, Synchrotron, X-ray imaging",

author = "Chien, {Chia Chi} and Kempson, {Ivan M.} and Wang, {Cheng Liang} and Hsianghisn Chen and Yeukuang Hwu and Chen, {Y. N.} and Ting-Kuo Lee and Tsai, {Kelvin K C} and Liu, {Ming Sheng} and Chang, {Kwang Yu} and Yang, {Chung Shi} and G. Margaritondo",

note = "Funding Information: This work was supported by the ANR-NSC French–Taiwan bilateral program no. ANR-09-BLAN-0385 , the National Science and Technology Program for Nanoscience and Nanotechnology , the Thematic Research Project of Academia Sinica , the Biomedical Nano-Imaging Core Facility at the National Synchrotron Radiation Research Center (Taiwan ), the National Health Research Institutes (NHRI) Intramural Research Program CA-099-PP-19 and the Department of Health (DOH) of Taiwan grant DOH99-TD-C-111-004 (to K.K.T), the Fonds National Suisse pour la Recherche Scientifique and the CIBM . ",

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doi = "10.1016/j.biotechadv.2011.12.001",

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AU - Chen, Hsianghisn

AU - Hwu, Yeukuang

AU - Chen, Y. N.

AU - Lee, Ting-Kuo

AU - Tsai, Kelvin K C

AU - Liu, Ming Sheng

AU - Chang, Kwang Yu

AU - Yang, Chung Shi

AU - Margaritondo, G.

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N2 - Complete profiling would substantially facilitate the fundamental understanding of tumor angiogenesis and of possible anti-angiogenesis cancer treatments. We developed an integrated synchrotron-based methodology with excellent performances: detection of very small vessels by high spatial resolution (~. 1. μm) and nanoparticle contrast enhancement, in vivo dynamics investigations with high temporal resolution (~. 1. ms), and three-dimensional quantitative morphology parametrization by computer tracing. The smallest (3-10. μm) microvessels were found to constitute >80% of the tumor vasculature and exhibit many structural anomalies. Practical applications are presented, including vessel microanalysis in xenografted tumors, monitoring the effects of anti-angiogenetic agents and in vivo detection of tumor vascular rheological properties.

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KW - Quantitative analysis

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KW - X-ray imaging

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Complete microscale profiling of tumor microangiogenesis. A microradiological methodology reveals fundamental aspects of tumor angiogenesis and yields an array of quantitative parameters for its characterization.

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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