Escherichia coli is the most common pathogen associated with acute lobar nephroma (ALN), a clinically more severe parenchymal inflammatory disease that requires a longer duration of antibiotic treatment than acute pyelonephritis (APN). This study was conducted to unravel the virulence differences between clinical isolates of E. coli from pediatric ALN and APN patients. A total of 88 urinary isolates of E. coli were investigated. They were identified from ra-diologically diagnosed ALN and APN patients and had previously been molecularly characterized for important urovirulence genes. Madin-Darby canine kidney (MDCK) epithelial cells were used as an in vitro model. Multivariate logistic regression analyses indicated that ALN isolates were more likely to show adhesion (p<0.05; odds ratio [OR], 3.81; 95% confidence interval [CI], 1.23-11.80) and cytotoxicity (p<0.001; OR, 10.42; 95% CI, 3.03-35.89). However, no difference in the penetration ability was noted. Henceforth, the ability to adhere to and produce cytotoxicity against uroepithelial cells appears a prerequisite factor for E. coli to cause more severe bacterial kidney infection, such as ALN.
|Number of pages||8|
|Publication status||Published - Jan 2011|
- Acute focal bacterial nephritis
- Bacterial adhesion
ASJC Scopus subject areas
- Microbiology (medical)