Comparison of cell response and surface characteristics on titanium implant with sla and slaffinity functionalization

Chiung Fang Huang, Hsi Jen Chiang, Hao Jan Lin, Hossein Hosseinkhani, Keng Liang Ou, Pei Wen Pengaz

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


To evaluate the cell responses of titanium oxide layer with the nanoporous micro-structure, an electrochemical anodization approach was proposed to create a nanoporous structure that superimposed onto the moderately rough sand-blasted, large-grit, acid-etched (SLA) Ti surface (denoted as SLAffinity-Ti). The osteoblast-like cells were cultured on SLAffinity-Ti and un-treated surfaces. Cell adhesion, viability, mRNA expressions as well as the productions of alkaline phosphatase (ALP) and osteocalcin (OC) were evaluated at predetermined intervals. The present results showed that cells had a high viability, attachment, and improved cell proliferation at day 7 and 14 when cultured on SLAffinity-Ti surface. The mRNA expressions of ALP and OC were upregulated while the type I collagen was not influenced. Additionally, the cells cultured on the SLAffinity-Ti surface produced a higher level of ALP and OC relative to cells on untreated surfaces. These findings confirmed that surface topography (nanoporous roughness) and TiO2 layers influenced the cell responses and the study revealed that cells grown on SLAffinity-Ti may improve osseointegration for Ti implants.

Original languageEnglish
Pages (from-to)G15-G20
JournalJournal of the Electrochemical Society
Issue number3
Publication statusPublished - 2014

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Renewable Energy, Sustainability and the Environment
  • Surfaces, Coatings and Films
  • Electrochemistry
  • Materials Chemistry


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