Comparison of an electrochemical biosensor with optical devices for hemoglobin measurement in human whole blood samples

Ming Song Hsieh, Tai Guang Wu, Chein Shyong Su, Wen Jing Cheng, Namik Ozbek, Kun Yuan Tsai, Ching Yu Lin

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Background: An electrochemical based biosensor for hemoglobin measurement was developed as an alternative to the traditional optical method, and underwent testing for use in professional settings. Methods: The affects of samples' freshness, hemolysis, bilirubin on the electrochemical method, as well as the repeatability, precision and accuracy were studied, using optical method devices as references. Results: Samples were stored at room temperature or in a cold environment for 7. days, partially or completely hemolyzed samples, and samples containing bilirubin with a concentration of up to 150. mg/l were investigated with no effects for interfering studies. Repeatability of finger blood testings was verified with six consecutive tests on nine volunteers, results ranged from 3% to 8% variation. The test results of BeneCheck were correlated with Sysmex, Beckman Coulters, Cell-Dyn and HemoCue methods, the results have shown similar and 95% of test results were within a ± 15% bias. Conclusions: BeneCheck hemoglobin test system performed well and accurately, while requiring 1μl of blood sample and 10. s detection time. Based on the cost, accuracy, sample volume, measuring time, ease of viewing and portability, BeneCheck deliver the best characteristics for these purposes.

Original languageEnglish
Pages (from-to)2150-2156
Number of pages7
JournalClinica Chimica Acta
Issue number23-24
Publication statusPublished - Nov 20 2011


  • Biosensor
  • Capillary blood
  • Electrochemical
  • Hemoglobin

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical


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