Abstract
The aim of this study is to evaluate the influence of serum [fetal bovine serum (FBS)] in cell culture medium on the nano and micro-sized ZnO toxicity to human lung epithelial cells (A549). Particles agglomeration and sedimentation state in medium were respectively measured by Zetasizer and UV-vis spectrometer. Methyl thiazolyl tetrazolium (MTT) and interleukin 8 (IL-8) analysis were selected for cytotoxicity assays. We found ZnO particles with a smaller primary size increased to a relative smaller secondary size in both serum/serum-free Dulbecco's modified Eagle's medium (DMEM), and caused the size-dependent cytotoxicity effect on ZnO nanoparticles. However, negative charges of serum caused the zeta potential of ZnO slightly decreased to -8 ~ -10 mV in DMEM, which resulted in smaller hydrodynamic size and much slower sedimentation than in serum free medium, leading to similarly lower toxicity.
| Original language | English |
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| Title of host publication | Nanotechnology 2010 |
| Subtitle of host publication | Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 |
| Pages | 557-560 |
| Number of pages | 4 |
| Volume | 3 |
| Publication status | Published - 2010 |
| Externally published | Yes |
| Event | Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, CA, United States Duration: Jun 21 2010 → Jun 24 2010 |
Conference
| Conference | Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 |
|---|---|
| Country/Territory | United States |
| City | Anaheim, CA |
| Period | 6/21/10 → 6/24/10 |
Keywords
- A549
- Cytotoxicity
- Nanoparticles
- Zinc oxide
ASJC Scopus subject areas
- Bioengineering
- Biomedical Engineering
- Electrical and Electronic Engineering