@inproceedings{8e3f7b19760640ea8f9679cebeb480d6,
title = "Comparing cytotoxicity of ZnO nanoparticles in exposure to serum/serum-free cell culture medium",
abstract = "The aim of this study is to evaluate the influence of serum [fetal bovine serum (FBS)] in cell culture medium on the nano and micro-sized ZnO toxicity to human lung epithelial cells (A549). Particles agglomeration and sedimentation state in medium were respectively measured by Zetasizer and UV-vis spectrometer. Methyl thiazolyl tetrazolium (MTT) and interleukin 8 (IL-8) analysis were selected for cytotoxicity assays. We found ZnO particles with a smaller primary size increased to a relative smaller secondary size in both serum/serum-free Dulbecco's modified Eagle's medium (DMEM), and caused the size-dependent cytotoxicity effect on ZnO nanoparticles. However, negative charges of serum caused the zeta potential of ZnO slightly decreased to -8 ~ -10 mV in DMEM, which resulted in smaller hydrodynamic size and much slower sedimentation than in serum free medium, leading to similarly lower toxicity.",
keywords = "A549, Cytotoxicity, Nanoparticles, Zinc oxide",
author = "Hsiao, {I. L.} and Huang, {Y. J.}",
year = "2010",
language = "English",
isbn = "9781439834152",
volume = "3",
series = "Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010",
pages = "557--560",
booktitle = "Nanotechnology 2010",
note = "Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 ; Conference date: 21-06-2010 Through 24-06-2010",
}