TY - JOUR
T1 - Combined impact of high-pressure processing and slightly acidic electrolysed water on Listeria monocytogenes proteomes
AU - Chen, Guan Wen
AU - Chen, Yi An
AU - Chang, Hsin Yi
AU - Huang, Tsui Chin
AU - Chen, Tai Yuan
N1 - Funding Information:
This work was supported by the Ministry of Science and Technology of Taiwan (105-2320-B-019-001-MY3). The funders had no role in research design, data analysis, the decision to publish, or preparation of the manuscript. Mass spectrometric protein identifications and analyses were performed by the Common Mass Spectrometry Facilities sponsored by the Institute of Biological Chemistry, Academia Sinica, Taiwan. This manuscript was edited by Wallace Academic Editing.
Funding Information:
This work was supported by the Ministry of Science and Technology of Taiwan (105-2320-B-019-001-MY3). The funders had no role in research design, data analysis, the decision to publish, or preparation of the manuscript. Mass spectrometric protein identifications and analyses were performed by the Common Mass Spectrometry Facilities sponsored by the Institute of Biological Chemistry, Academia Sinica, Taiwan. This manuscript was edited by Wallace Academic Editing.
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/9
Y1 - 2021/9
N2 - Slightly acidic electrolysed water (SAEW) and high-pressure processing (HPP) are well-established non-thermal preservation technologies. This study investigated the deactivation mechanisms of Listeria monocytogenes by label-free quantitative proteomics analysis. Samples were treated through HPP (300 MPa for 3 min), SAEW (20 ppm available chlorine concentration), and their combinations. The KEGG pathway analysis found SAEW + HPP induced differentially expressed proteins (DEPs) associated to biofunctions of ribosomes, secondary metabolite biosynthesis, microbial metabolism in diverse environments, carbon metabolism, and biosynthesis of amino acid and aminoacyl-transfer RNA. The results showed these non-thermal treatments were able to induce the shifting of ribosome biogenesis to initiate translation in L. monocytogenes. During protein translation, the initiation stage was upregulated. However, subsequent elongation, termination, and recycling of used ribosomes were retarded. Comparing various treatments, the combination of hurdles showed greater deactivation of L. monocytogenes than any single one. The approaches developed in this study provided crucial information for minimally processing in the food industries on the application of foodborne listeriosis prevention.
AB - Slightly acidic electrolysed water (SAEW) and high-pressure processing (HPP) are well-established non-thermal preservation technologies. This study investigated the deactivation mechanisms of Listeria monocytogenes by label-free quantitative proteomics analysis. Samples were treated through HPP (300 MPa for 3 min), SAEW (20 ppm available chlorine concentration), and their combinations. The KEGG pathway analysis found SAEW + HPP induced differentially expressed proteins (DEPs) associated to biofunctions of ribosomes, secondary metabolite biosynthesis, microbial metabolism in diverse environments, carbon metabolism, and biosynthesis of amino acid and aminoacyl-transfer RNA. The results showed these non-thermal treatments were able to induce the shifting of ribosome biogenesis to initiate translation in L. monocytogenes. During protein translation, the initiation stage was upregulated. However, subsequent elongation, termination, and recycling of used ribosomes were retarded. Comparing various treatments, the combination of hurdles showed greater deactivation of L. monocytogenes than any single one. The approaches developed in this study provided crucial information for minimally processing in the food industries on the application of foodborne listeriosis prevention.
KW - High pressure processing
KW - Listeria monocytogenes
KW - Non-thermal
KW - Proteomics
KW - Ribosomes
KW - Slightly acidic electrolysed water
KW - Stress response
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U2 - 10.1016/j.foodres.2021.110494
DO - 10.1016/j.foodres.2021.110494
M3 - Article
C2 - 34399490
AN - SCOPUS:85107998285
SN - 0963-9969
VL - 147
JO - Food Research International
JF - Food Research International
M1 - 110494
ER -