Abstract
Objectives: The aim of this study was to determine whether the oncogenic microRNA family members miR-196a and miR-196b can be circulating biomarkers for the early detection of oral cancer. Design and methods: To determine the stability of circulating miRNA, the blood sample was aliquot and stored at different temperature conditions for analysis. To assess the diagnostic efficacy, we determined the levels of miR-196s in plasma samples, including 53 from healthy individuals, 16 from pre-cancer patients, and 90 from oral cancer patients. Results: In general, circulating miRNA was very stable when storing plasma samples at -20. °C or below. In clinical study, both circulating miR-196a and miR-196b were substantially up-regulated in patients with oral pre-cancer lesions (5.9- and 14.8-fold, respectively; P
Original language | English |
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Pages (from-to) | 115-121 |
Number of pages | 7 |
Journal | Clinical Biochemistry |
Volume | 48 |
Issue number | 3 |
DOIs | |
Publication status | Published - Feb 1 2015 |
Externally published | Yes |
Keywords
- Circulating miRNA
- Early detection
- MiR-196a
- MiR-196b
- Oral cancer
ASJC Scopus subject areas
- Clinical Biochemistry
- Medicine(all)