Combined determination of circulating miR-196a and miR-196b levels produces high sensitivity and specificity for early detection of oral cancer

Ya Ching Lu; Joseph Tung Chieh Chang; Yu Chen Huang; Chi Che Huang; Wen Ho Chen; Li Yu Lee; Bing Shen Huang; Yin Ju Chen; Hsiao Fang Li; Ann Joy Cheng

doi:10.1016/j.clinbiochem.2014.11.020

Combined determination of circulating miR-196a and miR-196b levels produces high sensitivity and specificity for early detection of oral cancer

Ya Ching Lu, Joseph Tung Chieh Chang, Yu Chen Huang, Chi Che Huang, Wen Ho Chen, Li Yu Lee, Bing Shen Huang, Yin Ju Chen, Hsiao Fang Li, Ann Joy Cheng

Research output: Contribution to journal › Article › peer-review

76 Citations (Scopus)

Abstract

Objectives: The aim of this study was to determine whether the oncogenic microRNA family members miR-196a and miR-196b can be circulating biomarkers for the early detection of oral cancer. Design and methods: To determine the stability of circulating miRNA, the blood sample was aliquot and stored at different temperature conditions for analysis. To assess the diagnostic efficacy, we determined the levels of miR-196s in plasma samples, including 53 from healthy individuals, 16 from pre-cancer patients, and 90 from oral cancer patients. Results: In general, circulating miRNA was very stable when storing plasma samples at -20. °C or below. In clinical study, both circulating miR-196a and miR-196b were substantially up-regulated in patients with oral pre-cancer lesions (5.9- and 14.8-fold, respectively; P

Original language	English
Pages (from-to)	115-121
Number of pages	7
Journal	Clinical Biochemistry
Volume	48
Issue number	3
DOIs	https://doi.org/10.1016/j.clinbiochem.2014.11.020
Publication status	Published - Feb 1 2015
Externally published	Yes

Keywords

Circulating miRNA
Early detection
MiR-196a
MiR-196b
Oral cancer

ASJC Scopus subject areas

Clinical Biochemistry
Medicine(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.clinbiochem.2014.11.020

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Lu, Y. C., Chang, J. T. C., Huang, Y. C., Huang, C. C., Chen, W. H., Lee, L. Y., Huang, B. S., Chen, Y. J., Li, H. F., & Cheng, A. J. (2015). Combined determination of circulating miR-196a and miR-196b levels produces high sensitivity and specificity for early detection of oral cancer. Clinical Biochemistry, 48(3), 115-121. https://doi.org/10.1016/j.clinbiochem.2014.11.020

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title = "Combined determination of circulating miR-196a and miR-196b levels produces high sensitivity and specificity for early detection of oral cancer",

abstract = "Objectives: The aim of this study was to determine whether the oncogenic microRNA family members miR-196a and miR-196b can be circulating biomarkers for the early detection of oral cancer. Design and methods: To determine the stability of circulating miRNA, the blood sample was aliquot and stored at different temperature conditions for analysis. To assess the diagnostic efficacy, we determined the levels of miR-196s in plasma samples, including 53 from healthy individuals, 16 from pre-cancer patients, and 90 from oral cancer patients. Results: In general, circulating miRNA was very stable when storing plasma samples at -20. °C or below. In clinical study, both circulating miR-196a and miR-196b were substantially up-regulated in patients with oral pre-cancer lesions (5.9- and 14.8-fold, respectively; P",

keywords = "Circulating miRNA, Early detection, MiR-196a, MiR-196b, Oral cancer",

author = "Lu, {Ya Ching} and Chang, {Joseph Tung Chieh} and Huang, {Yu Chen} and Huang, {Chi Che} and Chen, {Wen Ho} and Lee, {Li Yu} and Huang, {Bing Shen} and Chen, {Yin Ju} and Li, {Hsiao Fang} and Cheng, {Ann Joy}",

year = "2015",

month = feb,

day = "1",

doi = "10.1016/j.clinbiochem.2014.11.020",

language = "English",

volume = "48",

pages = "115--121",

journal = "Clinical Biochemistry",

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T1 - Combined determination of circulating miR-196a and miR-196b levels produces high sensitivity and specificity for early detection of oral cancer

AU - Lu, Ya Ching

AU - Chang, Joseph Tung Chieh

AU - Huang, Yu Chen

AU - Huang, Chi Che

AU - Chen, Wen Ho

AU - Lee, Li Yu

AU - Huang, Bing Shen

AU - Chen, Yin Ju

AU - Li, Hsiao Fang

AU - Cheng, Ann Joy

PY - 2015/2/1

Y1 - 2015/2/1

N2 - Objectives: The aim of this study was to determine whether the oncogenic microRNA family members miR-196a and miR-196b can be circulating biomarkers for the early detection of oral cancer. Design and methods: To determine the stability of circulating miRNA, the blood sample was aliquot and stored at different temperature conditions for analysis. To assess the diagnostic efficacy, we determined the levels of miR-196s in plasma samples, including 53 from healthy individuals, 16 from pre-cancer patients, and 90 from oral cancer patients. Results: In general, circulating miRNA was very stable when storing plasma samples at -20. °C or below. In clinical study, both circulating miR-196a and miR-196b were substantially up-regulated in patients with oral pre-cancer lesions (5.9- and 14.8-fold, respectively; P

AB - Objectives: The aim of this study was to determine whether the oncogenic microRNA family members miR-196a and miR-196b can be circulating biomarkers for the early detection of oral cancer. Design and methods: To determine the stability of circulating miRNA, the blood sample was aliquot and stored at different temperature conditions for analysis. To assess the diagnostic efficacy, we determined the levels of miR-196s in plasma samples, including 53 from healthy individuals, 16 from pre-cancer patients, and 90 from oral cancer patients. Results: In general, circulating miRNA was very stable when storing plasma samples at -20. °C or below. In clinical study, both circulating miR-196a and miR-196b were substantially up-regulated in patients with oral pre-cancer lesions (5.9- and 14.8-fold, respectively; P

KW - Circulating miRNA

KW - Early detection

KW - MiR-196a

KW - MiR-196b

KW - Oral cancer

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Combined determination of circulating miR-196a and miR-196b levels produces high sensitivity and specificity for early detection of oral cancer

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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