@article{eaafffa28e5945dbbc77a8646e6e346f,
title = "Collagen 1A1 (COL1A1) is a reliable biomarker and putative therapeutic target for hepatocellular carcinogenesis and metastasis",
abstract = "Increasing evidence shows that hepatocellular carcinoma (HCC) is a principal cause of cancer-related mortality globally, especially among Asian and African populations. Collagen type I α1 (COL1A1) is the major component of type I collagen. While aberrant expression of COL1A1 and COL1A2 is implicated in numerous cancers, the differential role of COL1A1 in malignant, premalignant and normal tissues remains unclear, and its clinical significance in HCC has not been elucidated. In this study, using bioinformatics analysis of publicly-available HCC microarray data from Gene Expression Omnibus (GEO) and RNAseq data from The Cancer Genome Atlas (TCGA) database, we determined that COL1A1 is significantly upregulated in HCC tumor tissues in comparison to normal tissues. Our analysis also revealed that COL1A1 confers survival advantage and enhanced oncogenicity on HCC cells. Interestingly, the siRNA-mediated silencing of COL1A1 expression (siCOLIA1) suppressed HCC cells clonogenicity, motility, invasiveness and tumorsphere formation. Concomitantly, siCOL1A1 abrogated Slug-dependent epithelial-to-mesenchymal transition (EMT) and HCC stemness gene-signature, by attenuating expression of stemness markers SOX2, OCT4 and CD133. The present study provides some mechanistic insight into COL1A1 activity in HCC and highlights its putative role as an important diagnostic biomarker and potential therapeutic target in early development and metastasis of HCC.",
keywords = "COL1A1, EMT, Hepatocellular carcinoma, Metastasis, Stemnessk",
author = "Ma, {Hon Ping} and Chang, {Hang Lung} and Bamodu, {Oluwaseun Adebayo} and Yadav, {Vijesh Kumar} and Huang, {Ting Yi} and Wu, {Alexander T.H.} and Yeh, {Chi Tai} and Tsai, {Shin Han} and Lee, {Wei Hwa}",
note = "Funding Information: Supplementary Materials: The following are available online at www.mdpi.com/xxx/s1. Figure S1: Full-size blots of Figure 3A, Figure S2: Full-size blots of Figure 3D, Figure S3: Full-size blots of Figure 5C, Table S1: Figure S1: Full-size blots of Figure 3A, Figure S2: Full-size blots of Figure 3D, Figure S3: Full-size blots of Figure 5C, Western blot antibodies sheet. Table S1: Western blot antibodies sheet. Authors Contributions: H.-P.M. and H.-L.C.: Study conception and design, collection and assembly of data, data analysis and interpretation, and manuscript writing. O.A.B.: Study conception and design, collection and asassesmembblylyo of fddaatata,,d daattaaa annaallyyssiiss aanndd iinntteerrpprreettaattiioonn,, mmaannuussccrriipptt wwrritiitningg aanndd RReevvisiisoino.n V. V.K.K.Y..Y, .T, .T-.Y-Y.H.H.,.A, A.T.T.H.H.W.W.: .: Data analysis and interpretation. C.-T.Y., S.-H.T. and W.-H.L.: Study conception and design, data analysis and interpretation. All authors read and approved the final manuscript. Funding: This study was also supported by grants from Taipei Medical University (102TMU-SHH-02) to Funding: This study was also supported by grants from Taipei Medical University (102TMU-SHH-02) to Wei-Wei-Hwa Lee. Hwa Lee. Acknowledgments: The authors thank all research assistants of the Cancer Translational Research Laboratory and Acknowledgments: The authors thank all research assistants of the Cancer Translational Research Laboratory maonldecuCloarreaFnadcicleitlyl- bCaesnedtera,ssTaayisp.ei Medical University-Shuang Ho Hospital for their assistance with the flow Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2019",
month = jun,
day = "1",
doi = "10.3390/cancers11060786",
language = "English",
volume = "11",
journal = "Cancers",
issn = "2072-6694",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "6",
}
